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The map label for this gene is topB6B

Identifier: 15921560

GI number: 15921560

Start: 1293409

End: 1295007

Strand: Direct

Name: topB6B

Synonym: ST1294

Alternate gene names: 15921560

Gene position: 1293409-1295007 (Clockwise)

Preceding gene: 15921559

Following gene: 15921561

Centisome position: 48.0

GC content: 33.15

Gene sequence:

>1599_bases
ATGTCATCTGAGAAGTTTACTAGTATTTCTCCTGCTGAATTCTTTAAAAGGAATCCAGAGTTGGCTGGTTTTAGTAATCC
TGCAAGAGCCTTATATCAAACAGTCAGAGAATTAGTAGAAAATGCATTAGACGCAACTGACGTCCATAATATTTTACCTT
CAATTAAGATAATAATCGAGTTAGTAGATCCTCAGAAGCAAATATACAAAGTTAATGTAGAAGATAATGGAATTGGTATC
CCTCCTCATATAGTTCCTAATGCTTTTGGTAAAGTTCTTTATAGCTCTAAATACGTTTTAAGGCAAACAAGAGGTATGTA
TGGGCTCGGAGTTAAAGCAGCAGTTCTATATAGTCAAATGTATCAAGAAAGGCCAGTAGAAGTAGTCACTTCCCCTATAA
ATTCTAAAAGAATTTATTTCTTTAAATTAAAAATTGACGTAGTTAAAAATGAGCCTATAATTTTACAAAAGACTTCGGTA
GTTAATGAGAAGAATTGGCATGGTACTTCCGTTACACTTTATTTATATGCTGACTGGCAAAGAGCAAAACAAAAGATTTA
TGAATATGTAAAGAAAACGTACATTATTTCACCTTATGCTGAATTCTTTTTCAAAGATCCAGATAATAATGTAATTTACT
ATAAGAGATTAACAGATAAGATACCAGAACCTCCTAAAGAAGTTAAGCCTCACCCTTACGGTGTGGATATTGAACTAATA
AAATTCATGATTGTTAAGAAAGATAAACCAGTCCCAGTTAGAGATTTTCTGATTAACGAGTTTCAAAGCATAGGTGATGT
TACAGCGGATAAAATATTAGAGATGGCTAAGCTTCCCAAAGATAAGAAGACAACTGAATTAACTGACGAAGAAATTTCCA
GATTAGTTGAGGTAATGAAGAAGTTTGATGATTTTAGACCCCCTTCAGCAGAAGCCCTTTCAGTTATAGGAGAGGATTTA
ATTAAATTAGGATTGAAAAGTATATTTAACCCAGAATTTGCAGAAGCTATTACAAGAAAACCTAAAGCGTATCAAGGACA
TCCTTTCATAGTAGAGGCTGGTATAGCTTATGGTGGGGCAATACAGCCATCTCCAGAGCCAATAGTATTAAGATACGCTA
ATAAAATACCATTAATTTATGATGAAAAATCAGATGTTATTTGGAAAGTTGTTACAGAAGAAATGGACTGGAAAAGATAT
GGAATAGAGGAAGAACAACCTCCGCTAGTAGTTATGGTACATTTGTGCAGTACAAAAGTTCCTTATAGGAGCGCTGGTAA
AGAAAGTATTGCTGATGTTGAAGAAATAGAGAAAGAAATAAAACTTGCCCTAATGGATGTAGCTAGAAAATTAAAGAAAT
ACATAACAGAGAAAAGGAAAGAAGAAGAGGCAAAAAAACGTCTTATTACTTACCTAAAATATATCCCAGAAGTTAGTAGA
GGGCTAGCTCTATTCTTAGTAGGAGGAGATAAACAGAAAATAGGAGATGCATATTCAGATTTGAGAGAAAAACTACTTAA
AATAGCGTTGAATAAACTTGAGGTAAACGATAAAAAATTAGAGGAGGAAATACGTAATTATAAAGTAGAGGAGTTATGA

Upstream 100 bases:

>100_bases
CGAAAATGTTGTCATCTATGGAGATGCTACAATTATTTTGCCCATTTTGTCAGCATCTTTATTAGGTTAAGCGAATAAAA
TATTTGTATTGTGATGATTT

Downstream 100 bases:

>100_bases
GTAGTGAATTAACTTCTAAGGTGGATAAAGAAGCTAGGAAGAAGGCAGCTGACACTCTTAGGCAAGTTTTTGTAAAATTA
ATTGAGCAAATAAATAACTC

Product: DNA topoisomerase VI subunit B

Products: NA

Alternate protein names: Type II DNA topoisomerase VI subunit B; TopoVI-B

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSSEKFTSISPAEFFKRNPELAGFSNPARALYQTVRELVENALDATDVHNILPSIKIIIELVDPQKQIYKVNVEDNGIGI
PPHIVPNAFGKVLYSSKYVLRQTRGMYGLGVKAAVLYSQMYQERPVEVVTSPINSKRIYFFKLKIDVVKNEPIILQKTSV
VNEKNWHGTSVTLYLYADWQRAKQKIYEYVKKTYIISPYAEFFFKDPDNNVIYYKRLTDKIPEPPKEVKPHPYGVDIELI
KFMIVKKDKPVPVRDFLINEFQSIGDVTADKILEMAKLPKDKKTTELTDEEISRLVEVMKKFDDFRPPSAEALSVIGEDL
IKLGLKSIFNPEFAEAITRKPKAYQGHPFIVEAGIAYGGAIQPSPEPIVLRYANKIPLIYDEKSDVIWKVVTEEMDWKRY
GIEEEQPPLVVMVHLCSTKVPYRSAGKESIADVEEIEKEIKLALMDVARKLKKYITEKRKEEEAKKRLITYLKYIPEVSR
GLALFLVGGDKQKIGDAYSDLREKLLKIALNKLEVNDKKLEEEIRNYKVEEL

Sequences:

>Translated_532_residues
MSSEKFTSISPAEFFKRNPELAGFSNPARALYQTVRELVENALDATDVHNILPSIKIIIELVDPQKQIYKVNVEDNGIGI
PPHIVPNAFGKVLYSSKYVLRQTRGMYGLGVKAAVLYSQMYQERPVEVVTSPINSKRIYFFKLKIDVVKNEPIILQKTSV
VNEKNWHGTSVTLYLYADWQRAKQKIYEYVKKTYIISPYAEFFFKDPDNNVIYYKRLTDKIPEPPKEVKPHPYGVDIELI
KFMIVKKDKPVPVRDFLINEFQSIGDVTADKILEMAKLPKDKKTTELTDEEISRLVEVMKKFDDFRPPSAEALSVIGEDL
IKLGLKSIFNPEFAEAITRKPKAYQGHPFIVEAGIAYGGAIQPSPEPIVLRYANKIPLIYDEKSDVIWKVVTEEMDWKRY
GIEEEQPPLVVMVHLCSTKVPYRSAGKESIADVEEIEKEIKLALMDVARKLKKYITEKRKEEEAKKRLITYLKYIPEVSR
GLALFLVGGDKQKIGDAYSDLREKLLKIALNKLEVNDKKLEEEIRNYKVEEL
>Mature_531_residues
SSEKFTSISPAEFFKRNPELAGFSNPARALYQTVRELVENALDATDVHNILPSIKIIIELVDPQKQIYKVNVEDNGIGIP
PHIVPNAFGKVLYSSKYVLRQTRGMYGLGVKAAVLYSQMYQERPVEVVTSPINSKRIYFFKLKIDVVKNEPIILQKTSVV
NEKNWHGTSVTLYLYADWQRAKQKIYEYVKKTYIISPYAEFFFKDPDNNVIYYKRLTDKIPEPPKEVKPHPYGVDIELIK
FMIVKKDKPVPVRDFLINEFQSIGDVTADKILEMAKLPKDKKTTELTDEEISRLVEVMKKFDDFRPPSAEALSVIGEDLI
KLGLKSIFNPEFAEAITRKPKAYQGHPFIVEAGIAYGGAIQPSPEPIVLRYANKIPLIYDEKSDVIWKVVTEEMDWKRYG
IEEEQPPLVVMVHLCSTKVPYRSAGKESIADVEEIEKEIKLALMDVARKLKKYITEKRKEEEAKKRLITYLKYIPEVSRG
LALFLVGGDKQKIGDAYSDLREKLLKIALNKLEVNDKKLEEEIRNYKVEEL

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP

COG id: COG1389

COG function: function code L; DNA topoisomerase VI, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP6B_SULTO (Q971T2)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377229.1
- ProteinModelPortal:   Q971T2
- SMR:   Q971T2
- GeneID:   1459296
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1294
- NMPDR:   fig|273063.1.peg.1397
- HOGENOM:   HBG733142
- OMA:   PYASAGK
- ProtClustDB:   PRK04184
- BioCyc:   STOK273063:ST1294-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- HAMAP:   MF_00322
- InterPro:   IPR003594
- InterPro:   IPR008616
- InterPro:   IPR010979
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005734
- InterPro:   IPR015320
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF006553
- ProDom:   PD013790
- SMART:   SM00387
- TIGRFAMs:   TIGR01052

Pfam domain/function: PF05833 FbpA; PF02518 HATPase_c; PF09239 Topo-VIb_trans; SSF55874 ATP_bd_ATPase; SSF46946 Ribosomal_H2TH; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =5.99.1.3

Molecular weight: Translated: 61205; Mature: 61074

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKFTSISPAEFFKRNPELAGFSNPARALYQTVRELVENALDATDVHNILPSIKIIIE
CCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVDPQKQIYKVNVEDNGIGIPPHIVPNAFGKVLYSSKYVLRQTRGMYGLGVKAAVLYSQM
HCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
YQERPVEVVTSPINSKRIYFFKLKIDVVKNEPIILQKTSVVNEKNWHGTSVTLYLYADWQ
HHHCCHHHHCCCCCCCEEEEEEEEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEEEECHH
RAKQKIYEYVKKTYIISPYAEFFFKDPDNNVIYYKRLTDKIPEPPKEVKPHPYGVDIELI
HHHHHHHHHHHHHEEECCHHHHEEECCCCCEEEEEEHHHCCCCCHHHCCCCCCCCCHHHH
KFMIVKKDKPVPVRDFLINEFQSIGDVTADKILEMAKLPKDKKTTELTDEEISRLVEVMK
HHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KFDDFRPPSAEALSVIGEDLIKLGLKSIFNPEFAEAITRKPKAYQGHPFIVEAGIAYGGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCC
IQPSPEPIVLRYANKIPLIYDEKSDVIWKVVTEEMDWKRYGIEEEQPPLVVMVHLCSTKV
CCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCCCC
PYRSAGKESIADVEEIEKEIKLALMDVARKLKKYITEKRKEEEAKKRLITYLKYIPEVSR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLALFLVGGDKQKIGDAYSDLREKLLKIALNKLEVNDKKLEEEIRNYKVEEL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSEKFTSISPAEFFKRNPELAGFSNPARALYQTVRELVENALDATDVHNILPSIKIIIE
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVDPQKQIYKVNVEDNGIGIPPHIVPNAFGKVLYSSKYVLRQTRGMYGLGVKAAVLYSQM
HCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
YQERPVEVVTSPINSKRIYFFKLKIDVVKNEPIILQKTSVVNEKNWHGTSVTLYLYADWQ
HHHCCHHHHCCCCCCCEEEEEEEEEEEECCCCEEEEEHHHCCCCCCCCCEEEEEEEECHH
RAKQKIYEYVKKTYIISPYAEFFFKDPDNNVIYYKRLTDKIPEPPKEVKPHPYGVDIELI
HHHHHHHHHHHHHEEECCHHHHEEECCCCCEEEEEEHHHCCCCCHHHCCCCCCCCCHHHH
KFMIVKKDKPVPVRDFLINEFQSIGDVTADKILEMAKLPKDKKTTELTDEEISRLVEVMK
HHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KFDDFRPPSAEALSVIGEDLIKLGLKSIFNPEFAEAITRKPKAYQGHPFIVEAGIAYGGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCC
IQPSPEPIVLRYANKIPLIYDEKSDVIWKVVTEEMDWKRYGIEEEQPPLVVMVHLCSTKV
CCCCCCCEEEEECCCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCCCC
PYRSAGKESIADVEEIEKEIKLALMDVARKLKKYITEKRKEEEAKKRLITYLKYIPEVSR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLALFLVGGDKQKIGDAYSDLREKLLKIALNKLEVNDKKLEEEIRNYKVEEL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479