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The map label for this gene is mdh [H]

Identifier: 159185305

GI number: 159185305

Start: 2624795

End: 2625757

Strand: Reverse

Name: mdh [H]

Synonym: Atu2639

Alternate gene names: 159185305

Gene position: 2625757-2624795 (Counterclockwise)

Preceding gene: 159185306

Following gene: 15889893

Centisome position: 92.4

GC content: 60.33

Gene sequence:

>963_bases
ATGGCTCGCAAAAAAATTGCACTTATTGGTTCTGGCATGATCGGCGGCACGCTGGCGCATCTCGCCAGCCTGAAGGAACT
GGGCGATATCGTCCTCTTCGACATCGCCGACGGCATCCCGCAGGGCAAGGGTCTGGATATTGCCCAGTCCGGCCCGGTTG
AAGGCTTCAATGCAAAGCTCTCCGGCGCTTCCGATTACGCCGCCATCGAAGGCGCAGACGTCTGCATCGTCACCGCAGGT
GTCGCCCGCAAGCCCGGCATGAGCCGCGATGATCTTCTTGGCATCAACCTCAAGGTCATGGAACAGGTCGGCGCCGGCAT
CAAGAAATATGCTCCGAACGCTTTCGTGATCTGCATCACCAACCCGCTCGACGCCATGGTCTGGGCGCTGCAGAAGTTCT
CCGGCCTGCCGAAGAACAAGGTCGTCGGCATGGCTGGCGTTCTCGACAGCGCACGCTTCCGCCTGTTCCTTGCCGAAGAA
TTCAACGTTTCGGTCCAGGACGTCACCGCCTTCGTTCTCGGCGGTCATGGCGACACCATGGTGCCGCTCGCCCGTTATTC
CACCGTTGGCGGCGTTCCGCTTACCGATCTCGTCAAGATGGGCTGGTTGACCGCCGAACGTCTTGAGCAGATCATCCAGC
GCACCCGTGACGGCGGCGCGGAAATCGTCGGCCTCTTGAAGACCGGCTCGGCCTATTACGCGCCGGCCGCTTCGGCTATC
GAAATGGCCGAATCCTACCTCAAGGACAAGAAGCGCGTTCTGCCCGCTGCTGCCCACCTTTCGGGCCAGTATGGCGTAGA
CGACATGTATGTCGGCGTGCCCACCATCATCGGTGCCGGCGGTATCGAGCGCGTCATCGAGATCGAACTGAACAAGGAAG
AAGAAGCCGCCTTCCAGAAATCCGTCGGCGCTGTCGCTGGTCTTTGCGAAGCCTGCATCAACATCGCTCCGTCGCTGAAG
TAA

Upstream 100 bases:

>100_bases
TCTGTTGCCAATTTTGGCCAATGGGGCTAATCGAAGTGCGACAATTTGGCTGCCGGTTTGGCGCGGTAAAATTGAATTGA
AGCTCAAGAGGAAGCATTCG

Downstream 100 bases:

>100_bases
TAACTTCAAGCGGTTCCAAACAGGGATTATTCCATGAATATTCACGAATATCAGGCTAAGGCTCTTCTGAAGGGCTACGG
CGCGCCGGTTGCTGAAGGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARKKIALIGSGMIGGTLAHLASLKELGDIVLFDIADGIPQGKGLDIAQSGPVEGFNAKLSGASDYAAIEGADVCIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSARFRLFLAEE
FNVSVQDVTAFVLGGHGDTMVPLARYSTVGGVPLTDLVKMGWLTAERLEQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAESYLKDKKRVLPAAAHLSGQYGVDDMYVGVPTIIGAGGIERVIEIELNKEEEAAFQKSVGAVAGLCEACINIAPSLK

Sequences:

>Translated_320_residues
MARKKIALIGSGMIGGTLAHLASLKELGDIVLFDIADGIPQGKGLDIAQSGPVEGFNAKLSGASDYAAIEGADVCIVTAG
VARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSARFRLFLAEE
FNVSVQDVTAFVLGGHGDTMVPLARYSTVGGVPLTDLVKMGWLTAERLEQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAESYLKDKKRVLPAAAHLSGQYGVDDMYVGVPTIIGAGGIERVIEIELNKEEEAAFQKSVGAVAGLCEACINIAPSLK
>Mature_319_residues
ARKKIALIGSGMIGGTLAHLASLKELGDIVLFDIADGIPQGKGLDIAQSGPVEGFNAKLSGASDYAAIEGADVCIVTAGV
ARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSARFRLFLAEEF
NVSVQDVTAFVLGGHGDTMVPLARYSTVGGVPLTDLVKMGWLTAERLEQIIQRTRDGGAEIVGLLKTGSAYYAPAASAIE
MAESYLKDKKRVLPAAAHLSGQYGVDDMYVGVPTIIGAGGIERVIEIELNKEEEAAFQKSVGAVAGLCEACINIAPSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=32.4590163934426, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=32.4590163934426, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=31.0126582278481, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=31.0126582278481, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=32.2475570032573, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=32.2475570032573, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=29.7029702970297, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=30.6930693069307, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=30.6930693069307, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=31.6742081447964, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=32.6530612244898, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=32.6633165829146, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21735621, Length=292, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI103472011, Length=309, Percent_Identity=21.0355987055016, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=31.0810810810811, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=282, Percent_Identity=27.3049645390071, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17554310, Length=293, Percent_Identity=29.0102389078498, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=26.4214046822742, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=255, Percent_Identity=29.0196078431373, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=27.843137254902, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=302, Percent_Identity=27.8145695364238, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550422, Length=304, Percent_Identity=26.3157894736842, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=28.0254777070064, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24663595, Length=272, Percent_Identity=28.6764705882353, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24647881, Length=244, Percent_Identity=29.0983606557377, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33537; Mature: 33406

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALIGSGMIGGTLAHLASLKELGDIVLFDIADGIPQGKGLDIAQSGPVEGFNAKL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
SGASDYAAIEGADVCIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
CCCCCCEEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKNKVVGMAGVLDSARFRLFLAEEFNVSVQDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHEEEHHHHHCCCEEEEEEECCCCCCHHHEEEEEECCCCCCC
VPLARYSTVGGVPLTDLVKMGWLTAERLEQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
EMAESYLKDKKRVLPAAAHLSGQYGVDDMYVGVPTIIGAGGIERVIEIELNKEEEAAFQK
HHHHHHHHHHHHHCHHHHHCCCCCCCCCEEECHHHHHCCCCCCEEEEEEECCHHHHHHHH
SVGAVAGLCEACINIAPSLK
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARKKIALIGSGMIGGTLAHLASLKELGDIVLFDIADGIPQGKGLDIAQSGPVEGFNAKL
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
SGASDYAAIEGADVCIVTAGVARKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
CCCCCCEEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKNKVVGMAGVLDSARFRLFLAEEFNVSVQDVTAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHEEEHHHHHCCCEEEEEEECCCCCCHHHEEEEEECCCCCCC
VPLARYSTVGGVPLTDLVKMGWLTAERLEQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
EMAESYLKDKKRVLPAAAHLSGQYGVDDMYVGVPTIIGAGGIERVIEIELNKEEEAAFQK
HHHHHHHHHHHHHCHHHHHCCCCCCCCCEEECHHHHHCCCCCCEEEEEEECCHHHHHHHH
SVGAVAGLCEACINIAPSLK
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA