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The map label for this gene is trmH [H]

Identifier: 159184265

GI number: 159184265

Start: 332078

End: 332938

Strand: Direct

Name: trmH [H]

Synonym: Atu0341

Alternate gene names: 159184265

Gene position: 332078-332938 (Clockwise)

Preceding gene: 15887690

Following gene: 15887692

Centisome position: 11.69

GC content: 61.32

Gene sequence:

>861_bases
ATGACGAAGGACATGCGCGAAAACATCACGCGCCGGGTTGGCCAGGTCAAGGAAGTGACGAGCCTTGCCAATCCCATCAT
CAAGGACATCAAGGCGCTCACCCAGAAAAAGGGCAGGGAAGAGACCGGCACCTTCATGGCCGAAGGCCTGAAGCTGGTCA
TCGACGCGCTCGAACTCGGCTGGACGATCAAGACGCTGGTTTACGCCAAGGCCGCCAAGGGTAAGCCGCTGGTGGAGCAG
GCGGCGGTCAAGACCGTTGCGTCAGGTGGTCTGGTGCTCGAAGTCAGCGAGAAGGTCATCGCCTCGATCACCCGCCGCGA
CAATCCGCAGATGGTCGTCGGTATTTTCGAACAGAAATGGAAGCCGCTGAAGGATATCCGCCCTGAAAAGGGCGAGACCT
ATATCGCGCTCGACCGCGTACGCGATCCCGGCAATCTCGGCACCATCATCCGCACGGCGGATGCGGCCGGTGCCTCGGGC
GTCATTCTGGTGGGCGATTGCACCGATCCCTTCTCGCTGGAGACGGTGCGTGCCACCATGGGCTCGGTCTTTGCCACGCC
CGTCGCCCGCGCGTCGGTTGAGGAATTCCTCTCCTGGAAAAAATCGGCCCATGTCAGCGTCGTTGCCACACATCTTGCCG
GTTCCGTGGATTATCGGAAGATCGACTACGCCGGCAAACCGGTCGTGATTTTGATGGGCAACGAACAGTCTGGCCTGCCG
CCGGAACTGGCCGGCAAGGCGGACCAGCTGGCGCGCATTCCCCAGCAGGGCCGCGCCGATAGTCTCAACCTTGCCATCGC
AACCGCCGTCATGCTGTTTGAAGCACGGCGACATCTCCTCGAACTGGCACCGGTGACGTAA

Upstream 100 bases:

>100_bases
TTGTCGCATCCGGTGAACTGGTCATTCGCGAGGCGGGGCTTGACGGCAAGACGCCGGGCCGCGTGCTTTCCACATCGCTA
TTCAGCCGTTGGGAGCCGAA

Downstream 100 bases:

>100_bases
TGGCCAAAGCGGCATTGTTCTCGAAATTCGCACCGGCTTTTACGCTGATCGCCGCCGCACTGGTGCTGGATCAGATCGTC
AAGCAGCTGGTCGAGGCCTA

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTKDMRENITRRVGQVKEVTSLANPIIKDIKALTQKKGREETGTFMAEGLKLVIDALELGWTIKTLVYAKAAKGKPLVEQ
AAVKTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQKWKPLKDIRPEKGETYIALDRVRDPGNLGTIIRTADAAGASG
VILVGDCTDPFSLETVRATMGSVFATPVARASVEEFLSWKKSAHVSVVATHLAGSVDYRKIDYAGKPVVILMGNEQSGLP
PELAGKADQLARIPQQGRADSLNLAIATAVMLFEARRHLLELAPVT

Sequences:

>Translated_286_residues
MTKDMRENITRRVGQVKEVTSLANPIIKDIKALTQKKGREETGTFMAEGLKLVIDALELGWTIKTLVYAKAAKGKPLVEQ
AAVKTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQKWKPLKDIRPEKGETYIALDRVRDPGNLGTIIRTADAAGASG
VILVGDCTDPFSLETVRATMGSVFATPVARASVEEFLSWKKSAHVSVVATHLAGSVDYRKIDYAGKPVVILMGNEQSGLP
PELAGKADQLARIPQQGRADSLNLAIATAVMLFEARRHLLELAPVT
>Mature_285_residues
TKDMRENITRRVGQVKEVTSLANPIIKDIKALTQKKGREETGTFMAEGLKLVIDALELGWTIKTLVYAKAAKGKPLVEQA
AVKTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQKWKPLKDIRPEKGETYIALDRVRDPGNLGTIIRTADAAGASGV
ILVGDCTDPFSLETVRATMGSVFATPVARASVEEFLSWKKSAHVSVVATHLAGSVDYRKIDYAGKPVVILMGNEQSGLPP
ELAGKADQLARIPQQGRADSLNLAIATAVMLFEARRHLLELAPVT

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=299, Percent_Identity=26.4214046822742, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790083, Length=144, Percent_Identity=34.7222222222222, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24666840, Length=278, Percent_Identity=24.8201438848921, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 30895; Mature: 30764

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDMRENITRRVGQVKEVTSLANPIIKDIKALTQKKGREETGTFMAEGLKLVIDALELG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
WTIKTLVYAKAAKGKPLVEQAAVKTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQKW
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEEHHHCC
KPLKDIRPEKGETYIALDRVRDPGNLGTIIRTADAAGASGVILVGDCTDPFSLETVRATM
CCHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHH
GSVFATPVARASVEEFLSWKKSAHVSVVATHLAGSVDYRKIDYAGKPVVILMGNEQSGLP
HHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCEEECCCCCCEEEEECCCCCCCC
PELAGKADQLARIPQQGRADSLNLAIATAVMLFEARRHLLELAPVT
HHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKDMRENITRRVGQVKEVTSLANPIIKDIKALTQKKGREETGTFMAEGLKLVIDALELG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
WTIKTLVYAKAAKGKPLVEQAAVKTVASGGLVLEVSEKVIASITRRDNPQMVVGIFEQKW
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCEEEEEHHHCC
KPLKDIRPEKGETYIALDRVRDPGNLGTIIRTADAAGASGVILVGDCTDPFSLETVRATM
CCHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHH
GSVFATPVARASVEEFLSWKKSAHVSVVATHLAGSVDYRKIDYAGKPVVILMGNEQSGLP
HHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCEEECCCCCCEEEEECCCCCCCC
PELAGKADQLARIPQQGRADSLNLAIATAVMLFEARRHLLELAPVT
HHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]