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The map label for this gene is gshB

Identifier: 159184250

GI number: 159184250

Start: 302305

End: 303264

Strand: Direct

Name: gshB

Synonym: Atu0310

Alternate gene names: 159184250

Gene position: 302305-303264 (Clockwise)

Preceding gene: 15887659

Following gene: 159184251

Centisome position: 10.64

GC content: 59.48

Gene sequence:

>960_bases
ATGGCGAAGATCAAGAACGTCGCGATCCAGATGGATCATGTCTCCGGCATCAATATTGCCGGCGACTCCACCTTCGCCAT
CAGCCTTGAGGCGCAGGCGCGCGGATACCGGCTGTTCCATTATACGCCCGAGCGGCTTTCCATGCGCGATGGCAAGATTT
ACGCCACAGTCGAGCAGATGGAACTGCGCGACATCAAGGGCGATCATTTTTCCCTGTCGGAGCCGGAGCGGGTCGATCTT
TCGACCATGGATGTCATTCATTTGCGCCAGGACCCGCCCTTCGATATGGCCTACATCACCTCGACCCATCTCCTGGAGCG
GATTCATCCCAAGACGCTTGTTGTCAATGACCCCGCCTGGGTGCGTAACTCGCCGGAGAAGATTTTCGTCACCGAATTCG
CCGACCTGATGCCGAAGACGCTGATCACCAAGGACGTTTCCGAGATCGCCCGCTTCCGCAATGAGATGGGCGATATCATC
CTCAAGCCGCTCTACGGTAATGGCGGCGCGGGGGTCTTCCATTCGGCGCGTGATGACCGCAATTTCTCCTCCCTGCTGGA
GATGTTCGGCCAGATGTTCCGTGAGCCCTATATCGCCCAGGAATACCTGCCTGATGTCCGAAAGGGCGACAAGCGCATCC
TGCTGGTGGATGGCGAGCCGGTGGGTGCGATCAACCGCGTGCCGGCGGAAAACGATGCCCGCTCCAACATGCACGCCGGC
GGCCGGCCTGAGCCGACGGAACTGACGGCGCGGGAACAGGAAATCTGCCGCCGCATCGGTCCTGCCCTGCGCGAGCGCGG
TTTCCTCTTCGTCGGTATCGATGTGATCGGTGATTATATGACGGAAATCAACGTCACCTCACCGACCGGCATTCGCGAAG
TGCGTAAATTCGGCGGCGCCGATGTGGCGAGCCTGCTGTGGGATGCGATCGAGAAAAAACGCGACGCCCAGGATTTCTGA

Upstream 100 bases:

>100_bases
ATTTCCGGGGTCTCTTCCGGTCCTGCCGGGAAGCTTGAAAAGCGAGCAAGGGGCAGGGCGGACCGGCGAAGCGTCCGCAT
ACTGCAAGAGGAAGATGTGC

Downstream 100 bases:

>100_bases
AGCGATTTCAATCGCTCCCGGTTTGTTCCGTGCGTGCAACTCGATACAACTAAAATACATCGCCTGCGAGAAATTGTTCT
CTAGGTGTTCTTGTTTTATT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAKIKNVAIQMDHVSGINIAGDSTFAISLEAQARGYRLFHYTPERLSMRDGKIYATVEQMELRDIKGDHFSLSEPERVDL
STMDVIHLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITKDVSEIARFRNEMGDII
LKPLYGNGGAGVFHSARDDRNFSSLLEMFGQMFREPYIAQEYLPDVRKGDKRILLVDGEPVGAINRVPAENDARSNMHAG
GRPEPTELTAREQEICRRIGPALRERGFLFVGIDVIGDYMTEINVTSPTGIREVRKFGGADVASLLWDAIEKKRDAQDF

Sequences:

>Translated_319_residues
MAKIKNVAIQMDHVSGINIAGDSTFAISLEAQARGYRLFHYTPERLSMRDGKIYATVEQMELRDIKGDHFSLSEPERVDL
STMDVIHLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITKDVSEIARFRNEMGDII
LKPLYGNGGAGVFHSARDDRNFSSLLEMFGQMFREPYIAQEYLPDVRKGDKRILLVDGEPVGAINRVPAENDARSNMHAG
GRPEPTELTAREQEICRRIGPALRERGFLFVGIDVIGDYMTEINVTSPTGIREVRKFGGADVASLLWDAIEKKRDAQDF
>Mature_318_residues
AKIKNVAIQMDHVSGINIAGDSTFAISLEAQARGYRLFHYTPERLSMRDGKIYATVEQMELRDIKGDHFSLSEPERVDLS
TMDVIHLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITKDVSEIARFRNEMGDIIL
KPLYGNGGAGVFHSARDDRNFSSLLEMFGQMFREPYIAQEYLPDVRKGDKRILLVDGEPVGAINRVPAENDARSNMHAGG
RPEPTELTAREQEICRRIGPALRERGFLFVGIDVIGDYMTEINVTSPTGIREVRKFGGADVASLLWDAIEKKRDAQDF

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=42.1725239616613, Blast_Score=251, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_AGRT5 (Q8UII5)

Other databases:

- EMBL:   AE007869
- PIR:   AF2614
- PIR:   E97396
- RefSeq:   NP_353341.2
- ProteinModelPortal:   Q8UII5
- SMR:   Q8UII5
- STRING:   Q8UII5
- GeneID:   1132348
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0310
- eggNOG:   COG0189
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- PhylomeDB:   Q8UII5
- ProtClustDB:   PRK05246
- BioCyc:   ATUM176299-1:ATU0310-MONOMER
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 36053; Mature: 35921

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKNVAIQMDHVSGINIAGDSTFAISLEAQARGYRLFHYTPERLSMRDGKIYATVEQM
CCCCCEEEEEEECCCCCEECCCCEEEEEEEECCCCEEEEEECHHHHCCCCCEEEEEEEHH
ELRDIKGDHFSLSEPERVDLSTMDVIHLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAW
HHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFADLMPKTLITKDVSEIARFRNEMGDIILKPLYGNGGAGVFHSARDDR
HCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCC
NFSSLLEMFGQMFREPYIAQEYLPDVRKGDKRILLVDGEPVGAINRVPAENDARSNMHAG
CHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GRPEPTELTAREQEICRRIGPALRERGFLFVGIDVIGDYMTEINVTSPTGIREVRKFGGA
CCCCCCCCCHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCHHHHHHHHCCC
DVASLLWDAIEKKRDAQDF
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKIKNVAIQMDHVSGINIAGDSTFAISLEAQARGYRLFHYTPERLSMRDGKIYATVEQM
CCCCEEEEEEECCCCCEECCCCEEEEEEEECCCCEEEEEECHHHHCCCCCEEEEEEEHH
ELRDIKGDHFSLSEPERVDLSTMDVIHLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAW
HHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFADLMPKTLITKDVSEIARFRNEMGDIILKPLYGNGGAGVFHSARDDR
HCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCC
NFSSLLEMFGQMFREPYIAQEYLPDVRKGDKRILLVDGEPVGAINRVPAENDARSNMHAG
CHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GRPEPTELTAREQEICRRIGPALRERGFLFVGIDVIGDYMTEINVTSPTGIREVRKFGGA
CCCCCCCCCHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCHHHHHHHHCCC
DVASLLWDAIEKKRDAQDF
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194