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The map label for this gene is poxA [H]

Identifier: 15889813

GI number: 15889813

Start: 2527915

End: 2528979

Strand: Direct

Name: poxA [H]

Synonym: Atu2554

Alternate gene names: 15889813

Gene position: 2527915-2528979 (Clockwise)

Preceding gene: 159185264

Following gene: 15889814

Centisome position: 88.96

GC content: 60.66

Gene sequence:

>1065_bases
ATGCGCGCAAGCAACGAGACAATGTCGCCCTGGTGGACACCTGATGTCCACGCCGATCGCCGCGCTTTTCTCCTTGGCCG
CAACGCGATCCAGTCGGCCTTCCGCGGCTATTTCGCCCGTGCCGATTTTCTGGAAGTCGATACCGCGACCCTGCAGGTTT
CCCCCGGCAATGAAGCGCATCTGCACGCCTTTGCCACCGACGGCCTGACGCCGGGCGGCGAGGCCGTGCCGCTCTATCTC
CATACCTCGCCGGAATTCGCCTGCAAAAAGCTGCTCGCCGCCGGTGAAAAACGCATTTCCTGTTTCGCTCATGTTTACAG
GAACCGGGAAAGGGGCCCCCTGCACCACCCGGAATTCACCATGCTGGAATGGTATAGGGTCGGCGAAACCTATGAGAGCC
TGATGAAGGACAGCGCCAATCTTCTGGCGCTTGCGGCCGAAACAACCGGGACGAAACATTTCACCTATCGCGGCCGCAGT
GTCGATCCCTTCCTCGAGCCGGAACGTCTGAGCGTTGCCGAGGCCTTCGAACGTTATGCCGGTATCGATCTTCTGGCGAC
CATCGGCCGCAATGGCGAAACGGATGAACGGCACCTCGCCGCAAGCCTCACCAATGCCGGCATTCGTGTGGCCGATGACG
ATACCTGGGCGGATATGTTCAGCCGCGTCATCGTCGAGCGCGTGGAGCCGGAACTCGGTTTCGGCCGCGCAACCATTCTT
GATGAATATCCCACCAGCGAAGCGGCCCTTGCCCGCAAATCGCCTGGCGATGCCCGAGTGGCGGAACGCTTCGAGCTTTA
TGCCTGCGGCGTGGAACTTGCCAATGCCTTCGGTGAATTGACCGATGCTGCCGAACAGAGACGCCGTTTCGAAATGGAGA
TGGCCGAGAAACTGCGGGTTTATGGAGAGACCTATCCACTGGACGAGGATTTTCTGGCCGCACTTGCGATCATGCCCCCG
GCAAGCGGCATAGCGCTCGGTTTCGACCGGCTGGTGATGTTGGCAACCGGCGCACCCCGCATCGATCTCGTCATGTGGGC
GCCCGTCGCGGAATATGGACGATGA

Upstream 100 bases:

>100_bases
CGGTTTTCCGCCCGGAATTGCTTTAAATGATGCATTCAGGCGTTTTGCGGACTGTTTCCCCGCAATCGGGCGCCGCAGAG
AAGACAGAAAAGAACAGACG

Downstream 100 bases:

>100_bases
CAAGGTTTGAAACCATCAAGACACCGGAAGCGCTGCTCGAAGCGGGGCTGATCGAAGCAGAGGCGCTTGAGGGTCTGCGC
GCGGTGACGCAGCGTTATGC

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRASNETMSPWWTPDVHADRRAFLLGRNAIQSAFRGYFARADFLEVDTATLQVSPGNEAHLHAFATDGLTPGGEAVPLYL
HTSPEFACKKLLAAGEKRISCFAHVYRNRERGPLHHPEFTMLEWYRVGETYESLMKDSANLLALAAETTGTKHFTYRGRS
VDPFLEPERLSVAEAFERYAGIDLLATIGRNGETDERHLAASLTNAGIRVADDDTWADMFSRVIVERVEPELGFGRATIL
DEYPTSEAALARKSPGDARVAERFELYACGVELANAFGELTDAAEQRRRFEMEMAEKLRVYGETYPLDEDFLAALAIMPP
ASGIALGFDRLVMLATGAPRIDLVMWAPVAEYGR

Sequences:

>Translated_354_residues
MRASNETMSPWWTPDVHADRRAFLLGRNAIQSAFRGYFARADFLEVDTATLQVSPGNEAHLHAFATDGLTPGGEAVPLYL
HTSPEFACKKLLAAGEKRISCFAHVYRNRERGPLHHPEFTMLEWYRVGETYESLMKDSANLLALAAETTGTKHFTYRGRS
VDPFLEPERLSVAEAFERYAGIDLLATIGRNGETDERHLAASLTNAGIRVADDDTWADMFSRVIVERVEPELGFGRATIL
DEYPTSEAALARKSPGDARVAERFELYACGVELANAFGELTDAAEQRRRFEMEMAEKLRVYGETYPLDEDFLAALAIMPP
ASGIALGFDRLVMLATGAPRIDLVMWAPVAEYGR
>Mature_354_residues
MRASNETMSPWWTPDVHADRRAFLLGRNAIQSAFRGYFARADFLEVDTATLQVSPGNEAHLHAFATDGLTPGGEAVPLYL
HTSPEFACKKLLAAGEKRISCFAHVYRNRERGPLHHPEFTMLEWYRVGETYESLMKDSANLLALAAETTGTKHFTYRGRS
VDPFLEPERLSVAEAFERYAGIDLLATIGRNGETDERHLAASLTNAGIRVADDDTWADMFSRVIVERVEPELGFGRATIL
DEYPTSEAALARKSPGDARVAERFELYACGVELANAFGELTDAAEQRRRFEMEMAEKLRVYGETYPLDEDFLAALAIMPP
ASGIALGFDRLVMLATGAPRIDLVMWAPVAEYGR

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=339, Percent_Identity=26.8436578171091, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI5031815, Length=339, Percent_Identity=26.8436578171091, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI87082379, Length=319, Percent_Identity=38.5579937304075, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1790571, Length=338, Percent_Identity=31.0650887573964, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789256, Length=333, Percent_Identity=30.9309309309309, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994340, Length=336, Percent_Identity=29.4642857142857, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17535927, Length=336, Percent_Identity=29.4642857142857, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17535925, Length=336, Percent_Identity=29.4642857142857, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320242, Length=355, Percent_Identity=27.6056338028169, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640851, Length=331, Percent_Identity=25.9818731117825, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640849, Length=331, Percent_Identity=25.9818731117825, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39389; Mature: 39389

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASNETMSPWWTPDVHADRRAFLLGRNAIQSAFRGYFARADFLEVDTATLQVSPGNEAH
CCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCE
LHAFATDGLTPGGEAVPLYLHTSPEFACKKLLAAGEKRISCFAHVYRNRERGPLHHPEFT
EEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCH
MLEWYRVGETYESLMKDSANLLALAAETTGTKHFTYRGRSVDPFLEPERLSVAEAFERYA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHC
GIDLLATIGRNGETDERHLAASLTNAGIRVADDDTWADMFSRVIVERVEPELGFGRATIL
CCHHEECCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
DEYPTSEAALARKSPGDARVAERFELYACGVELANAFGELTDAAEQRRRFEMEMAEKLRV
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGETYPLDEDFLAALAIMPPASGIALGFDRLVMLATGAPRIDLVMWAPVAEYGR
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCEEEEEECCHHHHCC
>Mature Secondary Structure
MRASNETMSPWWTPDVHADRRAFLLGRNAIQSAFRGYFARADFLEVDTATLQVSPGNEAH
CCCCCCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCE
LHAFATDGLTPGGEAVPLYLHTSPEFACKKLLAAGEKRISCFAHVYRNRERGPLHHPEFT
EEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCH
MLEWYRVGETYESLMKDSANLLALAAETTGTKHFTYRGRSVDPFLEPERLSVAEAFERYA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHC
GIDLLATIGRNGETDERHLAASLTNAGIRVADDDTWADMFSRVIVERVEPELGFGRATIL
CCHHEECCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEE
DEYPTSEAALARKSPGDARVAERFELYACGVELANAFGELTDAAEQRRRFEMEMAEKLRV
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGETYPLDEDFLAALAIMPPASGIALGFDRLVMLATGAPRIDLVMWAPVAEYGR
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCEEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA