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The map label for this gene is thiC

Identifier: 41410011

GI number: 41410011

Start: 4375480

End: 4377102

Strand: Reverse

Name: thiC

Synonym: MAP3913c

Alternate gene names: 41410011

Gene position: 4377102-4375480 (Counterclockwise)

Preceding gene: 41410012

Following gene: 41410010

Centisome position: 90.63

GC content: 68.02

Gene sequence:

>1623_bases
ATGACCGCAATTGTCGAACCGAGCGTGACCACCGGGCCCATCGCGGGCAGCAGCAAGGTCTACCGCGAACTGGACGGGGT
GCCCGGTGCCCGGGTTCCGTTCCGCCGCGTCCACCTGTCCACCGGCGACCACTTCGACCTCTACGACACCTCCGGCCCCT
ACACCGATCCGGACGCGACGATCGACCTGACGGCCGGGCTGCCGGCGCGGCCGGGCCGGGTGCGCGACCGCGGCACCCAG
CTGCAGCGGGCCCGGGCCGGCGAGATCACCGCGGAGATGGCGTTCATCGCCGCCCGCGAGGGCATGCCGGCGGAGTTGGT
GCGCGACGAGGTCGCCCGCGGCCGCGCCGTGATCCCGGCCAACCACAACCATCCCGAGATCGAGCCGATGATCATCGGCA
AGGCGTTCGCCACCAAGGTCAATGCGAACATCGGCAACTCGGCGGTGACCAGCTCGATCGCCGAGGAGGTCGACAAGATG
GTGTGGGCCACCCGGTGGGGTGCGGACACCATCATGGACCTGTCCACCGGCAAGAACATCCACGAGACCCGCGAATGGAT
CCTGCGTAATTCGCCGGTGCCGGTCGGCACCGTACCGATCTATCAGGCGCTGGAGAAGGTCAAGGGCGATCCGACGCTGC
TGACCTGGGAGATCTACCGCGACACCGTGATCGAGCAGTGCGAGCAGGGCGTGGACTACATGACCGTGCACGCCGGTGTG
CTGCTGCGCTACGTGCCGCTGACCGCCAAGCGGGTCACCGGCATCGTGTCCCGCGGCGGGTCGATCATGGCCGCCTGGTG
CCTGGCGCATCACCGAGAGTCGTTCCTGTACACCAACTTCGACGAGCTGTGCGAGATCTTCGCCCGCTATGACGTCACCT
TCTCCCTGGGCGACGGCCTGCGGCCCGGCTCGATCGCCGACGCCAACGACGCCGCGCAGTTCGCCGAGCTGCGCACCCTG
GGCGAGCTGACCAAGATCGCGAAATCCCATGGCGTGCAAGTGATGATCGAAGGCCCCGGGCACGTTCCGATGCACAAGAT
CGTGGAGAACGTGCGGCTGGAGGAGGAGTGGTGCGAGGAGGCCCCGTTCTACACGCTGGGCCCGCTGGCCACCGACATCG
CCCCGGCCTACGACCACATCACCTCGGCGATCGGCGCGGCCATCATCGCCCAGGCGGGCACGGCGATGCTGTGCTACGTG
ACCCCGAAGGAGCACCTGGGGCTGCCGGACCGCAAGGACGTCAAGGACGGCGTGATCGCCTACAAGATCGCCGCGCACGC
CGGCGACCTGGCCAAGGGGCACCCGCACGCGCAGCAGCGCGATAATGCGTTGAGCCAGGCGCGTTTCGAGTTCCGCTGGA
ACGACCAGTTCGCGCTGTCGCTGGATCCCGACACCGCGCGGGAGTATCACGACGAGACGCTGCCCGCCGAGCCCGCCAAG
ACCGCGCACTTCTGCTCGATGTGCGGGCCGAAGTTCTGCTCGATGCGCATCACCCAGGACGTCCGCGACTATGCGGCCAA
GCACGGGCTGGACAGCGAGGAAGCGATCGAGGCGGCCATGGCCGACAAGTCTCGCGAGTTCGCCGAGCACGGCAACCGGG
TGTATCTACCGCTGGCACAGTGA

Upstream 100 bases:

>100_bases
CATCAGACGCGGGAGCGCACGCCAAGTGTGCTGAGAGGACGGCTCGGGGCCGTCGACCGTACGAACCTGACCGGGTAATG
CCGGCGTAGGGAGATGAAAA

Downstream 100 bases:

>100_bases
CGGGGCCGCCGCCGACGCTGCCACTGGCGCCGCCGGGCACCACGCCGCGCCGGGTGCTGTCCATCGCCGGATCCGACTCC
GGGGGTGGTGCGGGCATCCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MTAIVEPSVTTGPIAGSSKVYRELDGVPGARVPFRRVHLSTGDHFDLYDTSGPYTDPDATIDLTAGLPARPGRVRDRGTQ
LQRARAGEITAEMAFIAAREGMPAELVRDEVARGRAVIPANHNHPEIEPMIIGKAFATKVNANIGNSAVTSSIAEEVDKM
VWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTLLTWEIYRDTVIEQCEQGVDYMTVHAGV
LLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFDELCEIFARYDVTFSLGDGLRPGSIADANDAAQFAELRTL
GELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEEWCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYV
TPKEHLGLPDRKDVKDGVIAYKIAAHAGDLAKGHPHAQQRDNALSQARFEFRWNDQFALSLDPDTAREYHDETLPAEPAK
TAHFCSMCGPKFCSMRITQDVRDYAAKHGLDSEEAIEAAMADKSREFAEHGNRVYLPLAQ

Sequences:

>Translated_540_residues
MTAIVEPSVTTGPIAGSSKVYRELDGVPGARVPFRRVHLSTGDHFDLYDTSGPYTDPDATIDLTAGLPARPGRVRDRGTQ
LQRARAGEITAEMAFIAAREGMPAELVRDEVARGRAVIPANHNHPEIEPMIIGKAFATKVNANIGNSAVTSSIAEEVDKM
VWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTLLTWEIYRDTVIEQCEQGVDYMTVHAGV
LLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFDELCEIFARYDVTFSLGDGLRPGSIADANDAAQFAELRTL
GELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEEWCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYV
TPKEHLGLPDRKDVKDGVIAYKIAAHAGDLAKGHPHAQQRDNALSQARFEFRWNDQFALSLDPDTAREYHDETLPAEPAK
TAHFCSMCGPKFCSMRITQDVRDYAAKHGLDSEEAIEAAMADKSREFAEHGNRVYLPLAQ
>Mature_539_residues
TAIVEPSVTTGPIAGSSKVYRELDGVPGARVPFRRVHLSTGDHFDLYDTSGPYTDPDATIDLTAGLPARPGRVRDRGTQL
QRARAGEITAEMAFIAAREGMPAELVRDEVARGRAVIPANHNHPEIEPMIIGKAFATKVNANIGNSAVTSSIAEEVDKMV
WATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPIYQALEKVKGDPTLLTWEIYRDTVIEQCEQGVDYMTVHAGVL
LRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFDELCEIFARYDVTFSLGDGLRPGSIADANDAAQFAELRTLG
ELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEEWCEEAPFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVT
PKEHLGLPDRKDVKDGVIAYKIAAHAGDLAKGHPHAQQRDNALSQARFEFRWNDQFALSLDPDTAREYHDETLPAEPAKT
AHFCSMCGPKFCSMRITQDVRDYAAKHGLDSEEAIEAAMADKSREFAEHGNRVYLPLAQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=593, Percent_Identity=61.0455311973018, Blast_Score=699, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 59251; Mature: 59119

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVEPSVTTGPIAGSSKVYRELDGVPGARVPFRRVHLSTGDHFDLYDTSGPYTDPDAT
CCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCE
IDLTAGLPARPGRVRDRGTQLQRARAGEITAEMAFIAAREGMPAELVRDEVARGRAVIPA
EEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECC
NHNHPEIEPMIIGKAFATKVNANIGNSAVTSSIAEEVDKMVWATRWGADTIMDLSTGKNI
CCCCCCCCEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
HETREWILRNSPVPVGTVPIYQALEKVKGDPTLLTWEIYRDTVIEQCEQGVDYMTVHAGV
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCEEEHHHHH
LLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFDELCEIFARYDVTFSLGDGL
HHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCHHHHHHHHHHHCEEEECCCCC
RPGSIADANDAAQFAELRTLGELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEEWCEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHC
APFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPDRKDVKDGVIA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCEEE
YKIAAHAGDLAKGHPHAQQRDNALSQARFEFRWNDQFALSLDPDTAREYHDETLPAEPAK
EEEECCCCCCCCCCCCHHHHHHHHHHCEEEEEECCEEEEEECCHHHHHHCCCCCCCCCCH
TAHFCSMCGPKFCSMRITQDVRDYAAKHGLDSEEAIEAAMADKSREFAEHGNRVYLPLAQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
TAIVEPSVTTGPIAGSSKVYRELDGVPGARVPFRRVHLSTGDHFDLYDTSGPYTDPDAT
CEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCE
IDLTAGLPARPGRVRDRGTQLQRARAGEITAEMAFIAAREGMPAELVRDEVARGRAVIPA
EEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECC
NHNHPEIEPMIIGKAFATKVNANIGNSAVTSSIAEEVDKMVWATRWGADTIMDLSTGKNI
CCCCCCCCEEEECHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
HETREWILRNSPVPVGTVPIYQALEKVKGDPTLLTWEIYRDTVIEQCEQGVDYMTVHAGV
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCEEEHHHHH
LLRYVPLTAKRVTGIVSRGGSIMAAWCLAHHRESFLYTNFDELCEIFARYDVTFSLGDGL
HHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCHHHHHHHHHHHCEEEECCCCC
RPGSIADANDAAQFAELRTLGELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEEWCEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCHHHHHC
APFYTLGPLATDIAPAYDHITSAIGAAIIAQAGTAMLCYVTPKEHLGLPDRKDVKDGVIA
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCEEE
YKIAAHAGDLAKGHPHAQQRDNALSQARFEFRWNDQFALSLDPDTAREYHDETLPAEPAK
EEEECCCCCCCCCCCCHHHHHHHHHHCEEEEEECCEEEEEECCHHHHHHCCCCCCCCCCH
TAHFCSMCGPKFCSMRITQDVRDYAAKHGLDSEEAIEAAMADKSREFAEHGNRVYLPLAQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]