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The map label for this gene is gacA

Identifier: 52842852

GI number: 52842852

Start: 2993753

End: 2994430

Strand: Reverse

Name: gacA

Synonym: lpg2646

Alternate gene names: 52842852

Gene position: 2994430-2993753 (Counterclockwise)

Preceding gene: 224458591

Following gene: 52842851

Centisome position: 88.13

GC content: 38.79

Gene sequence:

>678_bases
TTGGTCAAGGAGCATTATTTGATTAAAGTATTAATTGTTGATGACCATGCATTGGTTAGAATGGGCATTCGACGATTGCT
TGAAGATATGTCGGATGTTGATGTAGTTGCAGATGCTGAAAGTGGTGAGCAGGCTTTAGCCTATGTAAAAACCCACTCCC
CTGATGTCGTATTATTAGATATGAAAATGCCTGGGATAGATGGGTGGGAAGTGACGCGTCGTTTAAAAAAAACAAACCCA
AATATTAAAGTCATAGCTGTTACAGCTATTTGCTCAGATCCTCTCCCTACTCGAGTATTGCAATTAGGTGCTATGGGATA
CCTTACTAAAGAGTCAGGGGCTGAAGAAATGGCTGCCGCAATTCGTAAAGTGGCTAAAGGTGAGAAATATCTTAGTGCCG
AGATAGCACAAAAAATGGCAATTAATAGTCTTCAGGAGTCTCAGGATTCCCCTTTTGATTTATTGTCTGAAAGAGAAATG
CAAGTCATGTTGATGATTACTAGCGGCATGAATGTACAAGACATTGCTGATAGATTATTTTTAAGTAGTAAAACCATCAA
TGGCTATCGATATAGGATGTTTGAAAAATTGGGGATTAAAAATGATGTGGAGCTTACTTATTTGGCTATGAAGCACCGCA
TTATTGAGCACCCCAATGATTTGTCACAAGATGATTAG

Upstream 100 bases:

>100_bases
ATTTTGTAAGACAGCTGGACTTGTAGATTAGAGTGACTATGTATTGTAATGATTATCGAGTCTTGTTAACATCGACCAAC
ACAGTTTGTTCATTGACTGA

Downstream 100 bases:

>100_bases
ATGTTAATTTCATGAATGACTTACAGCTTTCCGCTGAACTCGCTTTATTTTTAACGAAACTCCCAAGTGAACCGGGCATA
TACCGCATGTTAGACGAAGA

Product: response regulator GacA

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MVKEHYLIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNP
NIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREM
QVMLMITSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Sequences:

>Translated_225_residues
MVKEHYLIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNP
NIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREM
QVMLMITSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
>Mature_225_residues
MVKEHYLIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNP
NIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREM
QVMLMITSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=207, Percent_Identity=50.7246376811594, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=38, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=27.5510204081633, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=32.0388349514563, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=31.8627450980392, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788546, Length=206, Percent_Identity=28.1553398058252, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787473, Length=179, Percent_Identity=27.9329608938547, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787375, Length=128, Percent_Identity=28.90625, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25327; Mature: 25327

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEHYLIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLD
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCEEEEE
MKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAA
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHH
IRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMITSGMNVQDIADRLF
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHH
LSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
HHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVKEHYLIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLD
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCEEEEE
MKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAA
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECCCCHHHHHHH
IRKVAKGEKYLSAEIAQKMAINSLQESQDSPFDLLSEREMQVMLMITSGMNVQDIADRLF
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHH
LSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD
HHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]