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The map label for this gene is treZ [H]

Identifier: 39997454

GI number: 39997454

Start: 2577762

End: 2579654

Strand: Direct

Name: treZ [H]

Synonym: GSU2358

Alternate gene names: 39997454

Gene position: 2577762-2579654 (Clockwise)

Preceding gene: 39997453

Following gene: 308513318

Centisome position: 67.58

GC content: 66.83

Gene sequence:

>1893_bases
ATGACGCCGTGGTGCCTGGATATCGGAGCGACGCCCGTCACCGGAGGAGTTCACTTCAGGGTTTGGAGCCCCCTGGCCGA
TTCCGTCGAGGTCATCCGCTGTGCCCGGGGCGAAGAAGCCTCGTGCCCTCTTTCCCGGGTCGGCGAATACTTTCAGGGGA
CCATACCGGGCATGGGTCCGGGCGACTGTTACTGGTACCTGCTGGATGGCACCCTTCGCCGGCCAGACCCGGCATCCCGC
TGCCAACCCGGGGGAGTTCACGGCCCCTCCCAGGTCGTGGCGCCGGACAGCTTCGCCTGGGCCGATGGCCAGTGGCGGGG
AGTGGCGCTGGAGGAACTCATCACCTACGAACTGCACGTGGGGACCTTTACGCCCGAGGGGACCTTCGGTGCGGCCGTGG
AGCGGCTGGATTATTTGCAGGAACTGGGAATCACCGCCCTGGAGCTGATGCCGGTGGCCGAGTTTCCGGGCAGCCGCAAC
TGGGGGTACGACGGAGTATTCCCCTTTGCCCCCCACCACGCGTATGGCGGCCCCGACGGTCTCAAGTCGCTGGTGGATGC
GTGCCACCGGCGGGGGCTGGCCGTGATCCTGGACGTGGTTTACAACCACCTGGGCCCCGAGGGCAACTATCTTCACGCCT
TTGCCCCCTACTTCACCGATCACTACCGGACCCCCTGGGGGGATGCCGTCAATTTCGACGGACCCGGCAGCGACGGGGTG
CGCCACTACGTGATCGGCAACGCACTCCACTGGATAAGCGAATACCATCTGGACGGCCTGAGGCTCGACGCCATCCACGG
CATCTATGACTTCAGCGCCGGCCACATCCTGCGGGACATTACCCGGGCGGTCGACTCCCTGGCACGGCACCTGGGGCGGA
CGGTCCACGTGATCGCCGAGAGCGACCTGAACGATGTGCGGACCATCGCGCCGCCGGAGTCAGGCGGGCACGGCGTCCAC
ACCCAGTGGTGCGACGATTTCCACCACTCCCTGGTGACCCTCCTGACCGGCGAGCGGAATGGTTACTACGCCGATTTCGG
CCTGTTCAGCCACCTGGAGAAGGCGTTCCGCGAGGGGTTCGTCTACTCCGGAGAATACTCGCGCCACCGCAGACGCCGCC
ACGGCAGCCCATCAGGGGATCAGCCCCCGCGCCGGATGGTGGTCTTTTCCCAAAACCACGACCAGATCGGTAACCGGGCC
CTGGGGGACCGGCCATCGACCCGCCTCTCCCCGGAGCAGCTCAAGCTGGCCGCCGGCGCAGTCATACTCTCCCCCTACCT
GCCGCTCATCTTCATGGGAGAGGAGTACGGCGAGACTGCCCCGTTCCCCTACTTCACGAGCCATGGCGATCCGGCGCTGG
TTGAGGCGGTGCGCCGGGGACGGCGCGAAGAATTCGCCGCCTTCGGCTGGGAGGCGGAAGTCCCCGACCCCCAGGACGAA
GCGACATTCCGCTCCGCCCTGATCGATCCGGAACTTCGCTCCGTCGGCTGGCACCAGCAGGTATTCTCCTTCTACCGGGA
AGTGATCCGGCTCCGGCGGCAACTGCCCAGCTTCCGCCGCCCCGAGCGGGAGGAAATCCAGGTGACCGGCCTGGAAGCGG
ACAGGGTCGTGGTCGTCCGGCTCTGCCGGGGCCAGGCCGAACTCGTGGTCCTCTTTGCCCTGGCCGAAACATCCTGCCGC
GTTCGACTACCCTTCCGCCACGGACGTTACCGCAAACTCTTGGACTCGGCCGAACCGCGCTGGGGCGGACCGGGGGCACT
GGCGCCCGAAGCCCTCGACGGAGGGGATGAAGGGATCGGGATCACCCTCGCGCCGTTCAGCGTCGTTCTGTATGGCAGGG
ACCGAGACGACCGTCGCCGGAACGGCGGCCCGATGCCGACTGAAGAAGGGTGA

Upstream 100 bases:

>100_bases
CGCCTCCATCGGCGTTATGCCAGGGAGAAAATCGAAATACCATTTCCGATCCGGACCCTCCACATCAAGCAGGAGGCGCC
GGCACAGGAAGGGGACTGAG

Downstream 100 bases:

>100_bases
CCGAGGCTCAATCAAGGACTGTTATGGAACGATTCATCTGCATACATGGCCATTTCTACCAGCCCCCCCGGGAGAACCCC
TGGCTGGAGGCGGTGGAGAT

Product: isoamylase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTPWCLDIGATPVTGGVHFRVWSPLADSVEVIRCARGEEASCPLSRVGEYFQGTIPGMGPGDCYWYLLDGTLRRPDPASR
CQPGGVHGPSQVVAPDSFAWADGQWRGVALEELITYELHVGTFTPEGTFGAAVERLDYLQELGITALELMPVAEFPGSRN
WGYDGVFPFAPHHAYGGPDGLKSLVDACHRRGLAVILDVVYNHLGPEGNYLHAFAPYFTDHYRTPWGDAVNFDGPGSDGV
RHYVIGNALHWISEYHLDGLRLDAIHGIYDFSAGHILRDITRAVDSLARHLGRTVHVIAESDLNDVRTIAPPESGGHGVH
TQWCDDFHHSLVTLLTGERNGYYADFGLFSHLEKAFREGFVYSGEYSRHRRRRHGSPSGDQPPRRMVVFSQNHDQIGNRA
LGDRPSTRLSPEQLKLAAGAVILSPYLPLIFMGEEYGETAPFPYFTSHGDPALVEAVRRGRREEFAAFGWEAEVPDPQDE
ATFRSALIDPELRSVGWHQQVFSFYREVIRLRRQLPSFRRPEREEIQVTGLEADRVVVVRLCRGQAELVVLFALAETSCR
VRLPFRHGRYRKLLDSAEPRWGGPGALAPEALDGGDEGIGITLAPFSVVLYGRDRDDRRRNGGPMPTEEG

Sequences:

>Translated_630_residues
MTPWCLDIGATPVTGGVHFRVWSPLADSVEVIRCARGEEASCPLSRVGEYFQGTIPGMGPGDCYWYLLDGTLRRPDPASR
CQPGGVHGPSQVVAPDSFAWADGQWRGVALEELITYELHVGTFTPEGTFGAAVERLDYLQELGITALELMPVAEFPGSRN
WGYDGVFPFAPHHAYGGPDGLKSLVDACHRRGLAVILDVVYNHLGPEGNYLHAFAPYFTDHYRTPWGDAVNFDGPGSDGV
RHYVIGNALHWISEYHLDGLRLDAIHGIYDFSAGHILRDITRAVDSLARHLGRTVHVIAESDLNDVRTIAPPESGGHGVH
TQWCDDFHHSLVTLLTGERNGYYADFGLFSHLEKAFREGFVYSGEYSRHRRRRHGSPSGDQPPRRMVVFSQNHDQIGNRA
LGDRPSTRLSPEQLKLAAGAVILSPYLPLIFMGEEYGETAPFPYFTSHGDPALVEAVRRGRREEFAAFGWEAEVPDPQDE
ATFRSALIDPELRSVGWHQQVFSFYREVIRLRRQLPSFRRPEREEIQVTGLEADRVVVVRLCRGQAELVVLFALAETSCR
VRLPFRHGRYRKLLDSAEPRWGGPGALAPEALDGGDEGIGITLAPFSVVLYGRDRDDRRRNGGPMPTEEG
>Mature_629_residues
TPWCLDIGATPVTGGVHFRVWSPLADSVEVIRCARGEEASCPLSRVGEYFQGTIPGMGPGDCYWYLLDGTLRRPDPASRC
QPGGVHGPSQVVAPDSFAWADGQWRGVALEELITYELHVGTFTPEGTFGAAVERLDYLQELGITALELMPVAEFPGSRNW
GYDGVFPFAPHHAYGGPDGLKSLVDACHRRGLAVILDVVYNHLGPEGNYLHAFAPYFTDHYRTPWGDAVNFDGPGSDGVR
HYVIGNALHWISEYHLDGLRLDAIHGIYDFSAGHILRDITRAVDSLARHLGRTVHVIAESDLNDVRTIAPPESGGHGVHT
QWCDDFHHSLVTLLTGERNGYYADFGLFSHLEKAFREGFVYSGEYSRHRRRRHGSPSGDQPPRRMVVFSQNHDQIGNRAL
GDRPSTRLSPEQLKLAAGAVILSPYLPLIFMGEEYGETAPFPYFTSHGDPALVEAVRRGRREEFAAFGWEAEVPDPQDEA
TFRSALIDPELRSVGWHQQVFSFYREVIRLRRQLPSFRRPEREEIQVTGLEADRVVVVRLCRGQAELVVLFALAETSCRV
RLPFRHGRYRKLLDSAEPRWGGPGALAPEALDGGDEGIGITLAPFSVVLYGRDRDDRRRNGGPMPTEEG

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789839, Length=291, Percent_Identity=27.8350515463918, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI2367229, Length=329, Percent_Identity=28.8753799392097, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554896, Length=178, Percent_Identity=31.4606741573034, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32564391, Length=178, Percent_Identity=31.4606741573034, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320826, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 70096; Mature: 69965

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPWCLDIGATPVTGGVHFRVWSPLADSVEVIRCARGEEASCPLSRVGEYFQGTIPGMGP
CCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC
GDCYWYLLDGTLRRPDPASRCQPGGVHGPSQVVAPDSFAWADGQWRGVALEELITYELHV
CCEEEEEECCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCCCCCCCCEEHHHHHEEEEEE
GTFTPEGTFGAAVERLDYLQELGITALELMPVAEFPGSRNWGYDGVFPFAPHHAYGGPDG
CCCCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
LKSLVDACHRRGLAVILDVVYNHLGPEGNYLHAFAPYFTDHYRTPWGDAVNFDGPGSDGV
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCC
RHYVIGNALHWISEYHLDGLRLDAIHGIYDFSAGHILRDITRAVDSLARHLGRTVHVIAE
EEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
SDLNDVRTIAPPESGGHGVHTQWCDDFHHSLVTLLTGERNGYYADFGLFSHLEKAFREGF
CCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHCCC
VYSGEYSRHRRRRHGSPSGDQPPRRMVVFSQNHDQIGNRALGDRPSTRLSPEQLKLAAGA
EECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHCH
VILSPYLPLIFMGEEYGETAPFPYFTSHGDPALVEAVRRGRREEFAAFGWEAEVPDPQDE
HHHCCCCCEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCHH
ATFRSALIDPELRSVGWHQQVFSFYREVIRLRRQLPSFRRPEREEIQVTGLEADRVVVVR
HHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCCEEEEH
LCRGQAELVVLFALAETSCRVRLPFRHGRYRKLLDSAEPRWGGPGALAPEALDGGDEGIG
HHCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCE
ITLAPFSVVLYGRDRDDRRRNGGPMPTEEG
EEEECEEEEEECCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPWCLDIGATPVTGGVHFRVWSPLADSVEVIRCARGEEASCPLSRVGEYFQGTIPGMGP
CCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC
GDCYWYLLDGTLRRPDPASRCQPGGVHGPSQVVAPDSFAWADGQWRGVALEELITYELHV
CCEEEEEECCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCCCCCCCCEEHHHHHEEEEEE
GTFTPEGTFGAAVERLDYLQELGITALELMPVAEFPGSRNWGYDGVFPFAPHHAYGGPDG
CCCCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
LKSLVDACHRRGLAVILDVVYNHLGPEGNYLHAFAPYFTDHYRTPWGDAVNFDGPGSDGV
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCC
RHYVIGNALHWISEYHLDGLRLDAIHGIYDFSAGHILRDITRAVDSLARHLGRTVHVIAE
EEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEC
SDLNDVRTIAPPESGGHGVHTQWCDDFHHSLVTLLTGERNGYYADFGLFSHLEKAFREGF
CCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHCCC
VYSGEYSRHRRRRHGSPSGDQPPRRMVVFSQNHDQIGNRALGDRPSTRLSPEQLKLAAGA
EECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHCH
VILSPYLPLIFMGEEYGETAPFPYFTSHGDPALVEAVRRGRREEFAAFGWEAEVPDPQDE
HHHCCCCCEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCHH
ATFRSALIDPELRSVGWHQQVFSFYREVIRLRRQLPSFRRPEREEIQVTGLEADRVVVVR
HHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCCEEEEH
LCRGQAELVVLFALAETSCRVRLPFRHGRYRKLLDSAEPRWGGPGALAPEALDGGDEGIG
HHCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCE
ITLAPFSVVLYGRDRDDRRRNGGPMPTEEG
EEEECEEEEEECCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]