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The map label for this gene is eno

Identifier: 39997384

GI number: 39997384

Start: 2503169

End: 2504455

Strand: Direct

Name: eno

Synonym: GSU2286

Alternate gene names: 39997384

Gene position: 2503169-2504455 (Clockwise)

Preceding gene: 39997376

Following gene: 39997387

Centisome position: 65.63

GC content: 60.61

Gene sequence:

>1287_bases
ATGAGCGAAATCACTGATGTTTACGCGAGGGAGATCCTCGATTCCCGCGGCAATCCCACCCTGGAGGTGGAAGTTTTTCT
GGAGTCCGGCGTCATGGGCCGTGCGGCTGTTCCCTCCGGCGCCTCGACCGGTGAGCGGGAAGCTCTGGAACTGCGGGACG
GCGACGCCTCACGTTACCTGGGCAAGGGAGTTCTCAAGGCCGTCGACAACGTGAACGACATCATTGCCGAGCAACTCATC
GGCATGGAGGCCACCGACCAGGTGGGGATCGACCGGCGGATGCTGGAGCTCGACGGAACGGAATACAAGAGCACCCTGGG
GGCCAACGCCATTCTCGGCGTGTCGCTTGCCGTTGCCAAGGCCGCGGCCGAAGAGGTCGGCCTCCCCCTCTACCAGTACA
TCGGCGGCTGCAATGCCCGGGAGCTTCCCCTCCCCATGATGAACATCCTCAACGGCGGTGCCCACGCCGACAACAACGTC
GACATCCAGGAGTTCATGATCATGCCCGCCGGTGCCCGGAGCTTTTCCGAAGCCCTGCGGATGGGGGCCGAGGTGTTCCA
CGCACTGAAGAGCGTCCTGAAGGGTAAGGGCTACAATACTGCCGTTGGGGACGAAGGGGGCTTCGCACCGAACCTCAAGT
CGAACGAAGAGGCACTCGAAGTCATCATGGAAGCCATTGCCAAGGCCGGTTACAAGGCGGGTGAGGACATCCTCCTGGCC
CTCGACGTGGCGTCGTCGGAGCTGTTCAAGGACGGCAAGTACTTCCTTGAGAACGAGGCCAAACCCGAAAAAACCGCAGA
CGAGCTGATCGATTTCTATGAGAACCTGGTGAACAAGTATCCCATCATCTCCATCGAGGACGGCATGGCCGAGAACGACT
GGGAAGGATGGAAGAAGATCACCGACCGCCTCGGCAAACGGGTCCAACTGGTGGGAGACGACCTCTTCGTAACCAATACG
AAGATCCTCAAGGAGGGGATCAGCAAGGGTGTAGCCAACTCCATCCTCATCAAACTGAACCAGATCGGCACGCTCACCGA
AACCCTCGACGCCATCGAGACTGCCAAGCGCGCCGGGTACACCACCGTCATCTCCCACCGCTCCGGCGAAACCGAGGACA
CGACCCTTGCCGACCTGGCGGTGGCGGTGAACGCCGGACAGATCAAGACCGGCTCCCTGTGCCGGACCGACCGGGTGGCC
AAGTACAACCAGTTGCTCAGGATCGAGGATGAACTGGATGTGACCGCCCAGTTCCGCGGTAAGGATGTATTCTACAACCT
GCGTTGA

Upstream 100 bases:

>100_bases
GGATTAAAGGTGTTTCCATTTCATGAGGGCTCTGCTATATACTTTCCCTGTTTTGTCCAAGGTCAGACGACCTGTCACAT
TCTCAGCAAGGGGGCCATGA

Downstream 100 bases:

>100_bases
ACCGGCGCCGGCCGACACTTCTCAAACGTACAAAAGGCGGGGGCATCCCCGCCTTTTTACTTATGGCTACCGGCGACTGA
ATCGCCCGCTCAGGCAGACC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDASRYLGKGVLKAVDNVNDIIAEQLI
GMEATDQVGIDRRMLELDGTEYKSTLGANAILGVSLAVAKAAAEEVGLPLYQYIGGCNARELPLPMMNILNGGAHADNNV
DIQEFMIMPAGARSFSEALRMGAEVFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIAKAGYKAGEDILLA
LDVASSELFKDGKYFLENEAKPEKTADELIDFYENLVNKYPIISIEDGMAENDWEGWKKITDRLGKRVQLVGDDLFVTNT
KILKEGISKGVANSILIKLNQIGTLTETLDAIETAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEDELDVTAQFRGKDVFYNLR

Sequences:

>Translated_428_residues
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDASRYLGKGVLKAVDNVNDIIAEQLI
GMEATDQVGIDRRMLELDGTEYKSTLGANAILGVSLAVAKAAAEEVGLPLYQYIGGCNARELPLPMMNILNGGAHADNNV
DIQEFMIMPAGARSFSEALRMGAEVFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIAKAGYKAGEDILLA
LDVASSELFKDGKYFLENEAKPEKTADELIDFYENLVNKYPIISIEDGMAENDWEGWKKITDRLGKRVQLVGDDLFVTNT
KILKEGISKGVANSILIKLNQIGTLTETLDAIETAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEDELDVTAQFRGKDVFYNLR
>Mature_427_residues
SEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDASRYLGKGVLKAVDNVNDIIAEQLIG
MEATDQVGIDRRMLELDGTEYKSTLGANAILGVSLAVAKAAAEEVGLPLYQYIGGCNARELPLPMMNILNGGAHADNNVD
IQEFMIMPAGARSFSEALRMGAEVFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIAKAGYKAGEDILLAL
DVASSELFKDGKYFLENEAKPEKTADELIDFYENLVNKYPIISIEDGMAENDWEGWKKITDRLGKRVQLVGDDLFVTNTK
ILKEGISKGVANSILIKLNQIGTLTETLDAIETAKRAGYTTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAK
YNQLLRIEDELDVTAQFRGKDVFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=54.0603248259861, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.9721577726218, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=50.9302325581395, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=50.9302325581395, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.2616822429907, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=352, Percent_Identity=29.2613636363636, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI169201757, Length=352, Percent_Identity=29.2613636363636, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239744207, Length=352, Percent_Identity=29.2613636363636, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310129182, Length=223, Percent_Identity=27.3542600896861, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI310110045, Length=223, Percent_Identity=27.3542600896861, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI310120572, Length=223, Percent_Identity=27.3542600896861, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=65.4117647058824, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71995829, Length=438, Percent_Identity=52.7397260273973, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=52.7397260273973, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=44.6700507614213, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=50.6880733944954, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=437, Percent_Identity=50.5720823798627, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=437, Percent_Identity=50.5720823798627, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=437, Percent_Identity=50.1144164759725, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=49.5412844036697, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=52.6682134570766, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=52.6682134570766, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=52.6682134570766, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=52.6682134570766, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=52.6682134570766, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=52.6682134570766, Blast_Score=415, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_GEOSL (Q74AR6)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953335.1
- ProteinModelPortal:   Q74AR6
- SMR:   Q74AR6
- GeneID:   2686931
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2286
- NMPDR:   fig|243231.1.peg.2273
- TIGR:   GSU2286
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   GSUL243231:GSU_2286-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46442; Mature: 46311

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 338-338 BINDING 155-155 BINDING 164-164 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDASRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHEECCCCHHHHH
GKGVLKAVDNVNDIIAEQLIGMEATDQVGIDRRMLELDGTEYKSTLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCHHHHHHCCCHHHHHHHHHHH
AAAEEVGLPLYQYIGGCNARELPLPMMNILNGGAHADNNVDIQEFMIMPAGARSFSEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
MGAEVFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIAKAGYKAGEDILLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELFKDGKYFLENEAKPEKTADELIDFYENLVNKYPIISIEDGMAENDWEGWKKI
EECHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHH
TDRLGKRVQLVGDDLFVTNTKILKEGISKGVANSILIKLNQIGTLTETLDAIETAKRAGY
HHHHCCEEEEECCCEEECHHHHHHHHHHHHHHHHHEEEEHHCCHHHHHHHHHHHHHHCCC
TTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAKYNQLLRIEDELDVTAQFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECC
KDVFYNLR
CCEEEECC
>Mature Secondary Structure 
SEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDASRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHEECCCCHHHHH
GKGVLKAVDNVNDIIAEQLIGMEATDQVGIDRRMLELDGTEYKSTLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCHHHHHHCCCHHHHHHHHHHH
AAAEEVGLPLYQYIGGCNARELPLPMMNILNGGAHADNNVDIQEFMIMPAGARSFSEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
MGAEVFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIAKAGYKAGEDILLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELFKDGKYFLENEAKPEKTADELIDFYENLVNKYPIISIEDGMAENDWEGWKKI
EECHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHH
TDRLGKRVQLVGDDLFVTNTKILKEGISKGVANSILIKLNQIGTLTETLDAIETAKRAGY
HHHHCCEEEEECCCEEECHHHHHHHHHHHHHHHHHEEEEHHCCHHHHHHHHHHHHHHCCC
TTVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVAKYNQLLRIEDELDVTAQFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEEEECC
KDVFYNLR
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA