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The map label for this gene is aceF [H]

Identifier: 38234214

GI number: 38234214

Start: 1673085

End: 1675034

Strand: Direct

Name: aceF [H]

Synonym: DIP1639

Alternate gene names: 38234214

Gene position: 1673085-1675034 (Clockwise)

Preceding gene: 38234212

Following gene: 38234215

Centisome position: 67.23

GC content: 55.9

Gene sequence:

>1950_bases
ATGGCGCACTCCGTTGTAATGCCTGAACTGGGCGAATCCGTCACCGAAGGTACCATCACCCAGTGGCTGAAGTCCGTTGG
TGACGCGGTCACTGCCGACGAGCCGTTGCTCGAGGTATCTACTGACAAGGTCGACACTGAAGTTCCTTCGCCTGTCTCCG
GTGTCTTGCTTGAGATCAAGTTTGAGGAAGATGACACCGTAGACGTCGGAGACGTTATTGCGATTATTGGCGAAGAAGGC
GACGCTCCAGCTGCCAAGGAAGAGCCAGCAGAGGAGCCAAAAGCTGAGGCACCAAAGGCTGAAGCAGCTGACTCCACCGA
CGTAGTCATGCCTGAACTCGGCGAGTCCGTCACCGAAGGCACCATCACCCAGTGGCTGAAGTCCGTTGGCGACACCGTCG
ACGTTGACGAACCACTCCTCGAAGTCTCCACCGACAAGGTCGACACCGAAGTCCCATCCCCAGTCGCTGGCACCATCCTC
GAAATCCTCTTCAACGAAGACGACACCGTCGACGTCGGAGACGTAATCGTTCGCGTTGGCACCCCAGGATCTGCTCCAGC
AGCCAAGGAAGAGCCAGCCAAGGAGCCAAAAGCCGAGACACCAAAGGAAGAGCCAAAAGCTGAGGCACCAAAGGCTGAAG
CAGCTGACTCCACCGACGTAGTCATGCCTGAACTCGGCGAGTCCGTCACCGAAGGCACCATCACCCAGTGGCTGAAGTCC
GTTGGCGACACCGTCGACGTTGACGAACCACTCCTCGAAGTCTCCACCGACAAGGTCGACACCGAAGTCCCATCCCCAGT
CGCTGGCACCATCCTCGAAATCCTCTTCAACGAAGACGACACCGTCGACGTCGGAGACGTAATCGTTCGCGTTGGCACCC
CAGGATCTGCTCCAGCAGCCAAGGAAGAGCCAGCCAAGGAGCCAAAAGCCGAGACACCAAAGGAAGAGCCAAAAGCTGAG
GCACCAAAGGCTGAGCCAAAGAAGGAAGCTCCTGCCAAGACCATCAACAACGAGAACGTCCCTTACGTCACTCCGCTGGT
CCGCAAGCTGGCTGACAAGCACGGCGTTGATCTGACCACTGTTGAAGGCACTGGTATTGGTGGACGCATCCGCAAACAGG
ATGTTTTGGCTGCTGCTGGCGTTGGCGCAGCCCCTGCAGCACAGGTTGCTCCTGCCCAGCCAGTTTCTGCAGCATCCACC
AAGTCCGTGGATCCAGAGAAGCAGAAGCTGATCGGTACAACTCAGAAGGTCAACCGTATCCGAGAGATCACCGCGCGCAA
GACTGTGGAGGCTTTGCAGATTTCTGCTCAGCTCACCCAGCTGCATGAGGTCGATATGACTAAGGTTGCGGAACTGCGTA
AGGCCAACAAGCCAGCCTTCCAAGAAAAGCACGGTGTGAACCTCACCTACTTGCCATTCTTTGCAAAGGCAATTGTTGAG
GCTTTGGTTGCTCACCCGAACGTCAATGCGTCCTACAACGCTGAGACAAAGGAGATGACCTACCACGCTGATGTCAACCT
CTCCATCGCCGTTGACACCCCAGCTGGTCTGCTTACCCCTGTTATCCACAAGGCTCAGGAGTTGTCCTTGCCTGAGCTCG
CTAAGGCAATTGTCGATTTGGCTGACCGTGCTCGTAACAACAAGCTCAAGCCGAATGATCTTTCCGGTGGCACCTTCACC
ATTACCAACATTGGTTCTGAGGGCGCTCTCAGCGATACCCCAATCTTGGTTCCACCACAGGCAGGTATCTTGGGTACCGG
CGCTATTGTTAAGCGTCCAGTAGTCATCACGGAAGATGGCATCGATTCGATTGCTATTCGCCAGATGGTCTACTTGCCAC
TTAGCTACGATCACCAGATCGTCGATGGTGCCGATGCAGGTCGTTTCTTGACCACCATCAAGGATCGCCTTGAGACTGCA
AACTTCGAAGGCGACCTCGAACTCTCCTAG

Upstream 100 bases:

>100_bases
GCAATCTTGGGGCCATCCGAGTCACACTGTGCCGGACAAAACGGTATCCTCGTATGTTATTAAAGTTCTTAACACTCGAC
TTGCGAGGAGTCTTTACACA

Downstream 100 bases:

>100_bases
TTCGTAGCACGCTTTACAAACGCCTCGTGGCAGACCTTGCTTTCACAACAAGTCCTGACACGAGGCGTTTCGTTTTGTCC
TCGTTGCTTTAGTAATGGCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MAHSVVMPELGESVTEGTITQWLKSVGDAVTADEPLLEVSTDKVDTEVPSPVSGVLLEIKFEEDDTVDVGDVIAIIGEEG
DAPAAKEEPAEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKSVGDTVDVDEPLLEVSTDKVDTEVPSPVAGTIL
EILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKS
VGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAE
APKAEPKKEAPAKTINNENVPYVTPLVRKLADKHGVDLTTVEGTGIGGRIRKQDVLAAAGVGAAPAAQVAPAQPVSAAST
KSVDPEKQKLIGTTQKVNRIREITARKTVEALQISAQLTQLHEVDMTKVAELRKANKPAFQEKHGVNLTYLPFFAKAIVE
ALVAHPNVNASYNAETKEMTYHADVNLSIAVDTPAGLLTPVIHKAQELSLPELAKAIVDLADRARNNKLKPNDLSGGTFT
ITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVYLPLSYDHQIVDGADAGRFLTTIKDRLETA
NFEGDLELS

Sequences:

>Translated_649_residues
MAHSVVMPELGESVTEGTITQWLKSVGDAVTADEPLLEVSTDKVDTEVPSPVSGVLLEIKFEEDDTVDVGDVIAIIGEEG
DAPAAKEEPAEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKSVGDTVDVDEPLLEVSTDKVDTEVPSPVAGTIL
EILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKS
VGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAE
APKAEPKKEAPAKTINNENVPYVTPLVRKLADKHGVDLTTVEGTGIGGRIRKQDVLAAAGVGAAPAAQVAPAQPVSAAST
KSVDPEKQKLIGTTQKVNRIREITARKTVEALQISAQLTQLHEVDMTKVAELRKANKPAFQEKHGVNLTYLPFFAKAIVE
ALVAHPNVNASYNAETKEMTYHADVNLSIAVDTPAGLLTPVIHKAQELSLPELAKAIVDLADRARNNKLKPNDLSGGTFT
ITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVYLPLSYDHQIVDGADAGRFLTTIKDRLETA
NFEGDLELS
>Mature_648_residues
AHSVVMPELGESVTEGTITQWLKSVGDAVTADEPLLEVSTDKVDTEVPSPVSGVLLEIKFEEDDTVDVGDVIAIIGEEGD
APAAKEEPAEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKSVGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILE
ILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKSV
GDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVGTPGSAPAAKEEPAKEPKAETPKEEPKAEA
PKAEPKKEAPAKTINNENVPYVTPLVRKLADKHGVDLTTVEGTGIGGRIRKQDVLAAAGVGAAPAAQVAPAQPVSAASTK
SVDPEKQKLIGTTQKVNRIREITARKTVEALQISAQLTQLHEVDMTKVAELRKANKPAFQEKHGVNLTYLPFFAKAIVEA
LVAHPNVNASYNAETKEMTYHADVNLSIAVDTPAGLLTPVIHKAQELSLPELAKAIVDLADRARNNKLKPNDLSGGTFTI
TNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDGIDSIAIRQMVYLPLSYDHQIVDGADAGRFLTTIKDRLETAN
FEGDLELS

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=40.1785714285714, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI31711992, Length=570, Percent_Identity=28.7719298245614, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=29.4663573085847, Blast_Score=160, Evalue=5e-39,
Organism=Homo sapiens, GI203098816, Length=474, Percent_Identity=27.6371308016878, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=27.7899343544858, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=37.125748502994, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=36.6430260047281, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1786305, Length=648, Percent_Identity=33.641975308642, Blast_Score=225, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=33.4117647058824, Blast_Score=192, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=28.6052009456265, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=29.5711060948081, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=27.2435897435897, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=435, Percent_Identity=34.0229885057471, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=27.0509977827051, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=38.2978723404255, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=28.1030444964871, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24582497, Length=301, Percent_Identity=28.2392026578073, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129315, Length=301, Percent_Identity=28.9036544850498, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 68619; Mature: 68487

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSVVMPELGESVTEGTITQWLKSVGDAVTADEPLLEVSTDKVDTEVPSPVSGVLLEIK
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEE
FEEDDTVDVGDVIAIIGEEGDAPAAKEEPAEEPKAEAPKAEAADSTDVVMPELGESVTEG
ECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHH
TITQWLKSVGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVG
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCEEEEEC
TPGSAPAAKEEPAKEPKAETPKEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHH
VGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVGTPGSAPAA
HCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCCC
KEEPAKEPKAETPKEEPKAEAPKAEPKKEAPAKTINNENVPYVTPLVRKLADKHGVDLTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCEEEE
VEGTGIGGRIRKQDVLAAAGVGAAPAAQVAPAQPVSAASTKSVDPEKQKLIGTTQKVNRI
ECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHH
REITARKTVEALQISAQLTQLHEVDMTKVAELRKANKPAFQEKHGVNLTYLPFFAKAIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEHHHHHHHHHHH
ALVAHPNVNASYNAETKEMTYHADVNLSIAVDTPAGLLTPVIHKAQELSLPELAKAIVDL
HHHHCCCCCCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ADRARNNKLKPNDLSGGTFTITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDG
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCEECCHHHHCCEEEECCC
IDSIAIRQMVYLPLSYDHQIVDGADAGRFLTTIKDRLETANFEGDLELS
CCHHHHHHHHEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
AHSVVMPELGESVTEGTITQWLKSVGDAVTADEPLLEVSTDKVDTEVPSPVSGVLLEIK
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEE
FEEDDTVDVGDVIAIIGEEGDAPAAKEEPAEEPKAEAPKAEAADSTDVVMPELGESVTEG
ECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHH
TITQWLKSVGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVG
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCEEEEEC
TPGSAPAAKEEPAKEPKAETPKEEPKAEAPKAEAADSTDVVMPELGESVTEGTITQWLKS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHH
VGDTVDVDEPLLEVSTDKVDTEVPSPVAGTILEILFNEDDTVDVGDVIVRVGTPGSAPAA
HCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCCC
KEEPAKEPKAETPKEEPKAEAPKAEPKKEAPAKTINNENVPYVTPLVRKLADKHGVDLTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCEEEE
VEGTGIGGRIRKQDVLAAAGVGAAPAAQVAPAQPVSAASTKSVDPEKQKLIGTTQKVNRI
ECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHH
REITARKTVEALQISAQLTQLHEVDMTKVAELRKANKPAFQEKHGVNLTYLPFFAKAIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEHHHHHHHHHHH
ALVAHPNVNASYNAETKEMTYHADVNLSIAVDTPAGLLTPVIHKAQELSLPELAKAIVDL
HHHHCCCCCCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
ADRARNNKLKPNDLSGGTFTITNIGSEGALSDTPILVPPQAGILGTGAIVKRPVVITEDG
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCEECCHHHHCCEEEECCC
IDSIAIRQMVYLPLSYDHQIVDGADAGRFLTTIKDRLETANFEGDLELS
CCHHHHHHHHEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]