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The map label for this gene is lipB

Identifier: 38234215

GI number: 38234215

Start: 1675151

End: 1675903

Strand: Direct

Name: lipB

Synonym: DIP1640

Alternate gene names: 38234215

Gene position: 1675151-1675903 (Clockwise)

Preceding gene: 38234214

Following gene: 38234216

Centisome position: 67.31

GC content: 54.85

Gene sequence:

>753_bases
ATGACTGCTGTTCGAGATCCGTTCTTTCCAGCCGACCAATCCATCCGTGCTTCCCACAACCCCATAGAAGTGCGCGAACT
CGGCATGATGGATTATCAACGCGCATGGGATCTCCAAGCAGAGCTAAATAAGCAACGCCAACATGACGAAATCAATGACG
TCATTTTGGTGCTGGAGCACCCCAGTATCTACACCGCGGGCAAGCGCACGCAGCCTTCTGATCGCCCCCAGAACGGCCTA
CCGGTTATTGATGTGGATCGCGGGGGCCGGATCACGTGGCATGGTCCGGGGCAGCTGGTGATGTACCCGATCATCAAGCT
TGCCGAGCCCATCGATGTTGTCGATTATGTTCGCCGTGTAGAAGAAGCCCTGATTCACGTCATCCGTACCCATGGGATTC
CTAACGCAGGGCGTATCGACGGCCGCTCAGGAGTGTGGGTCCCAGGAAAAACTCCTGAAGAACACCGTAAGGTTGCCGCT
CTAGGTATTCGCATTGCCGGTGGCGTGACCATGCATGGTTTAGCTCTGAACTGTGACAATACGGTTGAATTCTATGATTA
CATCGTTCCGTGTGGCATCTCCGATGCTGGGGTGACCACGATGTCACAAGAAGTGGGAAATGATGTCACCACTCAGTCGA
TGACAGCTCCCCTACTTGCCGCCCTCGATGATGCGTTTGCTGGTCGGCTCGTTGTCGCCGATCATAGCTTTGCTTCGGCT
CCGGACCCTACGAAGATTCCACGACGACCCTAA

Upstream 100 bases:

>100_bases
CAAACGCCTCGTGGCAGACCTTGCTTTCACAACAAGTCCTGACACGAGGCGTTTCGTTTTGTCCTCGTTGCTTTAGTAAT
GGCGGACTATAGTGTGATCC

Downstream 100 bases:

>100_bases
TTGCAGATACGACAACAAATTAAGGGGTGCTTCTTGCTGGTTGTTTACGTTCGTGTCGACGACCACAGCAATCACGAGTA
CTCTGCAAAGGGTGACTAAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTAVRDPFFPADQSIRASHNPIEVRELGMMDYQRAWDLQAELNKQRQHDEINDVILVLEHPSIYTAGKRTQPSDRPQNGL
PVIDVDRGGRITWHGPGQLVMYPIIKLAEPIDVVDYVRRVEEALIHVIRTHGIPNAGRIDGRSGVWVPGKTPEEHRKVAA
LGIRIAGGVTMHGLALNCDNTVEFYDYIVPCGISDAGVTTMSQEVGNDVTTQSMTAPLLAALDDAFAGRLVVADHSFASA
PDPTKIPRRP

Sequences:

>Translated_250_residues
MTAVRDPFFPADQSIRASHNPIEVRELGMMDYQRAWDLQAELNKQRQHDEINDVILVLEHPSIYTAGKRTQPSDRPQNGL
PVIDVDRGGRITWHGPGQLVMYPIIKLAEPIDVVDYVRRVEEALIHVIRTHGIPNAGRIDGRSGVWVPGKTPEEHRKVAA
LGIRIAGGVTMHGLALNCDNTVEFYDYIVPCGISDAGVTTMSQEVGNDVTTQSMTAPLLAALDDAFAGRLVVADHSFASA
PDPTKIPRRP
>Mature_249_residues
TAVRDPFFPADQSIRASHNPIEVRELGMMDYQRAWDLQAELNKQRQHDEINDVILVLEHPSIYTAGKRTQPSDRPQNGLP
VIDVDRGGRITWHGPGQLVMYPIIKLAEPIDVVDYVRRVEEALIHVIRTHGIPNAGRIDGRSGVWVPGKTPEEHRKVAAL
GIRIAGGVTMHGLALNCDNTVEFYDYIVPCGISDAGVTTMSQEVGNDVTTQSMTAPLLAALDDAFAGRLVVADHSFASAP
DPTKIPRRP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=216, Percent_Identity=29.6296296296296, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI87081767, Length=187, Percent_Identity=32.620320855615, Blast_Score=91, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323268, Length=233, Percent_Identity=25.7510729613734, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355483, Length=220, Percent_Identity=32.7272727272727, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_CORDI (Q6NG87)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939982.1
- ProteinModelPortal:   Q6NG87
- SMR:   Q6NG87
- GeneID:   2648686
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1640
- NMPDR:   fig|257309.1.peg.1573
- HOGENOM:   HBG716160
- OMA:   TEDHERP
- PhylomeDB:   Q6NG87
- ProtClustDB:   PRK14345
- BioCyc:   CDIP257309:DIP1640-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27510; Mature: 27378

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVRDPFFPADQSIRASHNPIEVRELGMMDYQRAWDLQAELNKQRQHDEINDVILVLEH
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHCHHHHHHHHHCCCCCCCEEEEEEC
PSIYTAGKRTQPSDRPQNGLPVIDVDRGGRITWHGPGQLVMYPIIKLAEPIDVVDYVRRV
CCEEECCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEHHHHHHCCCCCHHHHHHHH
EEALIHVIRTHGIPNAGRIDGRSGVWVPGKTPEEHRKVAALGIRIAGGVTMHGLALNCDN
HHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHEEEEECCEEEEEEEECCCC
TVEFYDYIVPCGISDAGVTTMSQEVGNDVTTQSMTAPLLAALDDAFAGRLVVADHSFASA
CCEEEHEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
PDPTKIPRRP
CCCCCCCCCC
>Mature Secondary Structure 
TAVRDPFFPADQSIRASHNPIEVRELGMMDYQRAWDLQAELNKQRQHDEINDVILVLEH
CCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHCHHHHHHHHHCCCCCCCEEEEEEC
PSIYTAGKRTQPSDRPQNGLPVIDVDRGGRITWHGPGQLVMYPIIKLAEPIDVVDYVRRV
CCEEECCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEHHHHHHCCCCCHHHHHHHH
EEALIHVIRTHGIPNAGRIDGRSGVWVPGKTPEEHRKVAALGIRIAGGVTMHGLALNCDN
HHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHEEEEECCEEEEEEEECCCC
TVEFYDYIVPCGISDAGVTTMSQEVGNDVTTQSMTAPLLAALDDAFAGRLVVADHSFASA
CCEEEHEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
PDPTKIPRRP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910