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The map label for this gene is cobS

Identifier: 38234210

GI number: 38234210

Start: 1668881

End: 1669705

Strand: Direct

Name: cobS

Synonym: DIP1635

Alternate gene names: 38234210

Gene position: 1668881-1669705 (Clockwise)

Preceding gene: 38234209

Following gene: 38234212

Centisome position: 67.06

GC content: 51.15

Gene sequence:

>825_bases
ATGTCTGGCAAAGCATATTTTGTCAACGGTGAGCATGGGCCTGCGATTATCGAAGGCCCACTGACTGCACTGAATTGGTT
AACTATTTTACCTGTTCCCAGCGCCACGGCATTCGATCGTGTCACAGGCGGACGCGTTATGGCATCTGTGCCCTTTATTG
GCATTGTGTTAGGAATTGTTGGCGGCGCTATTGCGTTCGCTGCAACGTCGCTCGGGGTGGCGTCGATAGTCGCTGCGACG
GTCATCGTGTGTTTTTGGGAGCTATTTACACGATTTATGCATCTCGACGGGCTTGCCGACGTCTCCGATGCCTTAGGTTC
CTATGCACCACCCGCACGTGCACGTGAAATTATCGCGGACCCGGCAACAGGGCTGATCGGTATGGGCGCATGCCTGATTT
CAATTTTGATCCACATCAGTTCTTTCACTGCACTTCTCGATGCTCACCTCTGGTGGATGGTGATGATTACCCCGATGATC
GGACGGTTTTGTGCCATCTTCGGAGCTCATTCACGGCTCAAACCCATGAACCCTACTGGTTTTGGTGCAATGCTCATTGG
CACAGTGAAAACTCACACTATTATCGCGTGGTTATGTGTGCTCCTTGTAATATGCATCGGCGTTCCCCTCGCAATGGATC
GCGGTGAGCTGATCACTATAACTATCCTAGGGTTGCTGTGCTCAGTAACTTTAGCTTTGGTAGAAATTCGCCATCTCCAT
CGCCGGTTTGAAGGCATGAATGGCGATACCACCGGCTTTATCATGCATTCTGCGACTGCGCTTTGCGCATTAGTCTTTGC
TGTAGGCGTGGGCATAGTAGCTTAA

Upstream 100 bases:

>100_bases
GCTGCTCATATCATGACGGACATGGCCACTTTTGATACTGCGGGTGTTTCTGGCAAAGGTGCAGTCCCTGCCAATCGCTC
GATGAAACAGTAGTTTCTAT

Downstream 100 bases:

>100_bases
AACCGCAAAACTCCAGAGCCGACTCCCCCTGTTTTCAGAAGGAGTCGGCTCAATCTCGTTATAGAAGAGAAATAATTTAC
GCCTCTACGAGAGTGTGCAT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSGKAYFVNGEHGPAIIEGPLTALNWLTILPVPSATAFDRVTGGRVMASVPFIGIVLGIVGGAIAFAATSLGVASIVAAT
VIVCFWELFTRFMHLDGLADVSDALGSYAPPARAREIIADPATGLIGMGACLISILIHISSFTALLDAHLWWMVMITPMI
GRFCAIFGAHSRLKPMNPTGFGAMLIGTVKTHTIIAWLCVLLVICIGVPLAMDRGELITITILGLLCSVTLALVEIRHLH
RRFEGMNGDTTGFIMHSATALCALVFAVGVGIVA

Sequences:

>Translated_274_residues
MSGKAYFVNGEHGPAIIEGPLTALNWLTILPVPSATAFDRVTGGRVMASVPFIGIVLGIVGGAIAFAATSLGVASIVAAT
VIVCFWELFTRFMHLDGLADVSDALGSYAPPARAREIIADPATGLIGMGACLISILIHISSFTALLDAHLWWMVMITPMI
GRFCAIFGAHSRLKPMNPTGFGAMLIGTVKTHTIIAWLCVLLVICIGVPLAMDRGELITITILGLLCSVTLALVEIRHLH
RRFEGMNGDTTGFIMHSATALCALVFAVGVGIVA
>Mature_273_residues
SGKAYFVNGEHGPAIIEGPLTALNWLTILPVPSATAFDRVTGGRVMASVPFIGIVLGIVGGAIAFAATSLGVASIVAATV
IVCFWELFTRFMHLDGLADVSDALGSYAPPARAREIIADPATGLIGMGACLISILIHISSFTALLDAHLWWMVMITPMIG
RFCAIFGAHSRLKPMNPTGFGAMLIGTVKTHTIIAWLCVLLVICIGVPLAMDRGELITITILGLLCSVTLALVEIRHLHR
RFEGMNGDTTGFIMHSATALCALVFAVGVGIVA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CORDI (Q6NG92)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939977.1
- GeneID:   2648681
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1635
- NMPDR:   fig|257309.1.peg.1568
- HOGENOM:   HBG729392
- OMA:   SILIHIS
- ProtClustDB:   PRK00235
- BioCyc:   CDIP257309:DIP1635-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28824; Mature: 28693

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd6d6dd0)-; HASH(0x451d383c)-; HASH(0x4521dcd8)-; HASH(0x4522d680)-; HASH(0x451d18f8)-; HASH(0x4527fba4)-; HASH(0x4521b9e0)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKAYFVNGEHGPAIIEGPLTALNWLTILPVPSATAFDRVTGGRVMASVPFIGIVLGIV
CCCCEEEEECCCCCEEEECCHHHHHHHHEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
GGAIAFAATSLGVASIVAATVIVCFWELFTRFMHLDGLADVSDALGSYAPPARAREIIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCC
PATGLIGMGACLISILIHISSFTALLDAHLWWMVMITPMIGRFCAIFGAHSRLKPMNPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
FGAMLIGTVKTHTIIAWLCVLLVICIGVPLAMDRGELITITILGLLCSVTLALVEIRHLH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRFEGMNGDTTGFIMHSATALCALVFAVGVGIVA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SGKAYFVNGEHGPAIIEGPLTALNWLTILPVPSATAFDRVTGGRVMASVPFIGIVLGIV
CCCEEEEECCCCCEEEECCHHHHHHHHEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
GGAIAFAATSLGVASIVAATVIVCFWELFTRFMHLDGLADVSDALGSYAPPARAREIIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCC
PATGLIGMGACLISILIHISSFTALLDAHLWWMVMITPMIGRFCAIFGAHSRLKPMNPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
FGAMLIGTVKTHTIIAWLCVLLVICIGVPLAMDRGELITITILGLLCSVTLALVEIRHLH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRFEGMNGDTTGFIMHSATALCALVFAVGVGIVA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14602910