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The map label for this gene is cobQ

Identifier: 38234074

GI number: 38234074

Start: 1517640

End: 1519079

Strand: Reverse

Name: cobQ

Synonym: DIP1495

Alternate gene names: 38234074

Gene position: 1519079-1517640 (Counterclockwise)

Preceding gene: 38234075

Following gene: 38234071

Centisome position: 61.04

GC content: 50.97

Gene sequence:

>1440_bases
ATGAAATCGTTCCTCGTCGCTGGATGTACGTCGGATGCTGGTAAATCTGTTGTTGTTGCAGGGCTATGTCGTGCGTTGAC
GAGGAGAGGTTTGCGTGTCGCCCCCTTTAAAGCACAAAACATGTCGAATAATTCGGCAGTTACTCCTGACGGAGGGGAGA
TCGGCAGGGCTCAAGCATTGCAAGCATATGCGTGCGGGCTTACCCCCAGCGTTGATTTCAACCCGATCTTGCTAAAACCA
GGATCAGATCGAACGTCACAGCTCGTTGTTCGAGGTATTGCCACAGGTAATGTATCCGCTCGCAGCTATATCGAGCACCG
CACTGAGCTTCGAAAAATTGCTTCCGAATCGCTTAACTCGCTTCGTGAGCGTTTCGACGTCGTTGTGTGTGAAGGTGCAG
GTTCTCCTGCTGAAACCAACCTGAGGGCGACAGATGTCGCTAATTTTGGGCTGGCGGAGGAATGCGATCTGCCTGTGTAT
ATTGTGGGTGACATTGATCGTGGTGGGGTACTGGCACATTTTTATGGAACTCATCAAATTGTTGATGATGCAGACCGCGC
TCGCATCAAGGGGTTTGTTGTCAATAAATTCCGTGGCGATGTGTCGATTCTTGAACCTGGCCTGCAAGACCTAGAAGATC
GTCTCGGAGTCCCTACAGTGGCCGTACTTCCGTTTATCCATGGTTTGTGGATTGATGCTGAAGATTCTTTACAGTCCACA
ATCGGTGCAACCGTTGGCCCAGCAGCTGCGCCACTGGGTACACAACGATTGCGCGTCGCTGCTATTCGGCTACCTCGTGT
TTCCAACGCTACAGATGTTGAAGCCCTAGCTTGTGAGCCCGGAGTCACTGTAACGTGGACAGTTGATCCGGATTCGGTCG
CAGAAGCCGATTTAGTGGTCATCCCTGGGTCTAAGGCAACCCTTAGCGATTTAGCCTGGCTGCGTTCTACAGGTGTCGCT
GACGCAATAATGGAACGTGCACGGTCACAGCGACCAATTTTAGGTATTTGTGGCGGCTTTCAAATGATGTGTTCACACAT
TGACGATCCAGTGGAATCGGGAAGTACTACGCCCGTGGAAGGCCTGGGGATTTTTGACGTTGATATTGAGTTCCATCCTG
AAAAGACACTGATTCGTCACGAAAACGGTGGATATGAAGTCCACCATGGTCGTGTTGAACGAAGCACCGAGCAGCCATGG
ATTGGTAACGAAGGTGCACGAACGCAAAGCAACTTTGGTACTCATCGACATGGATATTTGGAAGATGACGAAGCGCGACG
TGATTTCCTTGGCACAGTGTCTACACTCGCACAACGCCCAGGCTTTGTAGTCGCTCCCGCAACTTCTTTTGAAGCGGAAC
GCAACAAGCAGCTCGACGTGATAGCCGATGCCATCGAACAACATTGGGATCTTGACCAACTCATTGCAGCGCTTACCTAA

Upstream 100 bases:

>100_bases
TTCTCAGCATCCCAGACGATGACGTTAAAGTATTCTAATCTCAGTTGTGCATAACCATGACGGTTGTTTTTCTGCCTAAA
TAAGGGGGAGTAGCTACATC

Downstream 100 bases:

>100_bases
AGAATAAAGCGAAGGGCTGTACAACAAAATAGTTGTACAGCCCTTCGCTTTATGCGATTAGGAGAAATCGAGTCCGAGCA
GTGCGTTTTCTGTTAGCTCA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKSFLVAGCTSDAGKSVVVAGLCRALTRRGLRVAPFKAQNMSNNSAVTPDGGEIGRAQALQAYACGLTPSVDFNPILLKP
GSDRTSQLVVRGIATGNVSARSYIEHRTELRKIASESLNSLRERFDVVVCEGAGSPAETNLRATDVANFGLAEECDLPVY
IVGDIDRGGVLAHFYGTHQIVDDADRARIKGFVVNKFRGDVSILEPGLQDLEDRLGVPTVAVLPFIHGLWIDAEDSLQST
IGATVGPAAAPLGTQRLRVAAIRLPRVSNATDVEALACEPGVTVTWTVDPDSVAEADLVVIPGSKATLSDLAWLRSTGVA
DAIMERARSQRPILGICGGFQMMCSHIDDPVESGSTTPVEGLGIFDVDIEFHPEKTLIRHENGGYEVHHGRVERSTEQPW
IGNEGARTQSNFGTHRHGYLEDDEARRDFLGTVSTLAQRPGFVVAPATSFEAERNKQLDVIADAIEQHWDLDQLIAALT

Sequences:

>Translated_479_residues
MKSFLVAGCTSDAGKSVVVAGLCRALTRRGLRVAPFKAQNMSNNSAVTPDGGEIGRAQALQAYACGLTPSVDFNPILLKP
GSDRTSQLVVRGIATGNVSARSYIEHRTELRKIASESLNSLRERFDVVVCEGAGSPAETNLRATDVANFGLAEECDLPVY
IVGDIDRGGVLAHFYGTHQIVDDADRARIKGFVVNKFRGDVSILEPGLQDLEDRLGVPTVAVLPFIHGLWIDAEDSLQST
IGATVGPAAAPLGTQRLRVAAIRLPRVSNATDVEALACEPGVTVTWTVDPDSVAEADLVVIPGSKATLSDLAWLRSTGVA
DAIMERARSQRPILGICGGFQMMCSHIDDPVESGSTTPVEGLGIFDVDIEFHPEKTLIRHENGGYEVHHGRVERSTEQPW
IGNEGARTQSNFGTHRHGYLEDDEARRDFLGTVSTLAQRPGFVVAPATSFEAERNKQLDVIADAIEQHWDLDQLIAALT
>Mature_479_residues
MKSFLVAGCTSDAGKSVVVAGLCRALTRRGLRVAPFKAQNMSNNSAVTPDGGEIGRAQALQAYACGLTPSVDFNPILLKP
GSDRTSQLVVRGIATGNVSARSYIEHRTELRKIASESLNSLRERFDVVVCEGAGSPAETNLRATDVANFGLAEECDLPVY
IVGDIDRGGVLAHFYGTHQIVDDADRARIKGFVVNKFRGDVSILEPGLQDLEDRLGVPTVAVLPFIHGLWIDAEDSLQST
IGATVGPAAAPLGTQRLRVAAIRLPRVSNATDVEALACEPGVTVTWTVDPDSVAEADLVVIPGSKATLSDLAWLRSTGVA
DAIMERARSQRPILGICGGFQMMCSHIDDPVESGSTTPVEGLGIFDVDIEFHPEKTLIRHENGGYEVHHGRVERSTEQPW
IGNEGARTQSNFGTHRHGYLEDDEARRDFLGTVSTLAQRPGFVVAPATSFEAERNKQLDVIADAIEQHWDLDQLIAALT

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CORDI (Q6NGL6)

Other databases:

- EMBL:   BX248358
- RefSeq:   NP_939841.1
- ProteinModelPortal:   Q6NGL6
- GeneID:   2650379
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1495
- NMPDR:   fig|257309.1.peg.1432
- HOGENOM:   HBG302271
- ProtClustDB:   PRK00784
- BioCyc:   CDIP257309:DIP1495-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51479; Mature: 51479

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 337-337 ACT_SITE 417-417

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFLVAGCTSDAGKSVVVAGLCRALTRRGLRVAPFKAQNMSNNSAVTPDGGEIGRAQAL
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QAYACGLTPSVDFNPILLKPGSDRTSQLVVRGIATGNVSARSYIEHRTELRKIASESLNS
HHHHCCCCCCCCCCEEEECCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LRERFDVVVCEGAGSPAETNLRATDVANFGLAEECDLPVYIVGDIDRGGVLAHFYGTHQI
HHHHHCEEEECCCCCCCCCCCEEEHHCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCHH
VDDADRARIKGFVVNKFRGDVSILEPGLQDLEDRLGVPTVAVLPFIHGLWIDAEDSLQST
HCCCCHHHHEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHEEECCHHHHHHH
IGATVGPAAAPLGTQRLRVAAIRLPRVSNATDVEALACEPGVTVTWTVDPDSVAEADLVV
HCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCEEE
IPGSKATLSDLAWLRSTGVADAIMERARSQRPILGICGGFQMMCSHIDDPVESGSTTPVE
ECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHCCCCCCCC
GLGIFDVDIEFHPEKTLIRHENGGYEVHHGRVERSTEQPWIGNEGARTQSNFGTHRHGYL
CCEEEEEEEEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EDDEARRDFLGTVSTLAQRPGFVVAPATSFEAERNKQLDVIADAIEQHWDLDQLIAALT
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKSFLVAGCTSDAGKSVVVAGLCRALTRRGLRVAPFKAQNMSNNSAVTPDGGEIGRAQAL
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QAYACGLTPSVDFNPILLKPGSDRTSQLVVRGIATGNVSARSYIEHRTELRKIASESLNS
HHHHCCCCCCCCCCEEEECCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LRERFDVVVCEGAGSPAETNLRATDVANFGLAEECDLPVYIVGDIDRGGVLAHFYGTHQI
HHHHHCEEEECCCCCCCCCCCEEEHHCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCHH
VDDADRARIKGFVVNKFRGDVSILEPGLQDLEDRLGVPTVAVLPFIHGLWIDAEDSLQST
HCCCCHHHHEEEEEECCCCCHHHHCCCHHHHHHHHCCCHHHHHHHHHHEEECCHHHHHHH
IGATVGPAAAPLGTQRLRVAAIRLPRVSNATDVEALACEPGVTVTWTVDPDSVAEADLVV
HCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCEEE
IPGSKATLSDLAWLRSTGVADAIMERARSQRPILGICGGFQMMCSHIDDPVESGSTTPVE
ECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHCCCCCCCC
GLGIFDVDIEFHPEKTLIRHENGGYEVHHGRVERSTEQPWIGNEGARTQSNFGTHRHGYL
CCEEEEEEEEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EDDEARRDFLGTVSTLAQRPGFVVAPATSFEAERNKQLDVIADAIEQHWDLDQLIAALT
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHCCCCHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910