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The map label for this gene is pyrB

Identifier: 15674868

GI number: 15674868

Start: 682214

End: 683149

Strand: Direct

Name: pyrB

Synonym: SPy_0832

Alternate gene names: 15674868

Gene position: 682214-683149 (Clockwise)

Preceding gene: 15674867

Following gene: 15674869

Centisome position: 36.83

GC content: 39.42

Gene sequence:

>936_bases
ATGTCAGTTGTAAATAATCGCGTTGCACTTACCAATTTAGTTTCAATGGAAGCATTAACCACCGAAGAAGTTTTAGGTCT
TATCAACCGTGGTTCAGAATATAAGGCTGGTAAAGTTGTTATTTCGGATCATCAAAAGGATCTTGTTGCCAATTTATTCT
TTGAAAACTCCACCCGAACCCATAAGTCTTTTGAAGTGGCTGAGAAGAAACTCGGCTTGACAGTTCTTGATTTCAATGCT
GATGCAAGTGCGGTTAACAAGGGAGAAAGTTTGTATGATACTGTGTTAACCATGAGTGCACTTGGAACAGACATCTGTGT
GATTCGTCACCCAGAAGATGATTATTACAAGGAATTAGTAGAAAGTCCAACTATTACAGCTTCCATTGTCAATGGAGGAG
ATGGTTCTGGACAACACCCGAGTCAATGTTTACTTGACTTATTAACCATCTATGAAGAATTTGGACGCTTTGAAGGCTTA
AAGATTGCGATCGCTGGTGACTTAACTCACTCTAGGGTGGCAAAGTCCAACATGCAAATCCTCAAAAGATTAGGAGCAGA
GCTTTATTTCTATGGACCTGAAGAATGGTATTCAGAAGCCTTTAATGCTTACGGCACTTATATTGCTATTGACCAGATTA
TTAAGGAATTGGATGTTCTTATGTTGCTGCGTGTACAGCACGAACGTCATGACGGCCATCAGAGTTTTTCAAAAGAAGGT
TACCATCAAGCGTTTGGATTGACACAGGAACGTTACCAACAATTAAAAGATTCTGCTATTATTATGCATCCAGCTCCAGT
TAATCGGGATGTGGAAATTGCAGATAGTTTGGTGGAAGCTCCTAAAGCGAGAATTGTATCTCAGATGGCTAATGGTGTCT
TTGTTCGCATGGCTATTATTGAAGCCATTTTGAATGGTAGAAATAAAAACTCATGA

Upstream 100 bases:

>100_bases
CCTTGGCATCATCTTATATCAGGTACTTCCTGAAAAATAGGAAGAATTGTTGTTAGACCACTTACATAGTCATCAGTCGT
TAGTGATAAAGGGGATAATC

Downstream 100 bases:

>100_bases
TGAAAGATTAAAGCATGTTTAGAAGGTAGGAAAAGGTAGGAAAATGACAAAACGATTACTTATTTTAGAAGACGGCACTA
TTTTTGAAGGAGAACCCTTT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNA
DASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLMLLRVQHERHDGHQSFSKEG
YHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS

Sequences:

>Translated_311_residues
MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNA
DASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLMLLRVQHERHDGHQSFSKEG
YHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS
>Mature_310_residues
SVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNAD
ASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGLK
IAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLMLLRVQHERHDGHQSFSKEGY
HQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=267, Percent_Identity=35.2059925093633, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI2367364, Length=270, Percent_Identity=34.4444444444444, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=287, Percent_Identity=33.1010452961672, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=32.4723247232472, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_STRP1 (P65620)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269042.1
- RefSeq:   YP_282004.1
- ProteinModelPortal:   P65620
- SMR:   P65620
- EnsemblBacteria:   EBSTRT00000001455
- EnsemblBacteria:   EBSTRT00000028543
- GeneID:   3572270
- GeneID:   900999
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0832
- KEGG:   spz:M5005_Spy_0641
- GeneTree:   EBGT00050000027081
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   SPYO160490:SPY0832-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0641-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34616; Mature: 34485

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
HKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELV
HHHHHHHHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
ESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGLKIAIAGDLTHSRVAKSNMQI
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLMLLRVQHERHDGHQSFSKEG
HHHCCCCEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
YHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAII
HHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EAILNGRNKNS
HHHHCCCCCCC
>Mature Secondary Structure 
SVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDHQKDLVANLFFENSTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCC
HKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALGTDICVIRHPEDDYYKELV
HHHHHHHHHHCCCEEEECCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
ESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGLKIAIAGDLTHSRVAKSNMQI
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELDVLMLLRVQHERHDGHQSFSKEG
HHHCCCCEEEECHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
YHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADSLVEAPKARIVSQMANGVFVRMAII
HHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EAILNGRNKNS
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296