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The map label for this gene is carA

Identifier: 15674869

GI number: 15674869

Start: 683193

End: 684275

Strand: Direct

Name: carA

Synonym: SPy_0833

Alternate gene names: 15674869

Gene position: 683193-684275 (Clockwise)

Preceding gene: 15674868

Following gene: 15674870

Centisome position: 36.88

GC content: 39.98

Gene sequence:

>1083_bases
ATGACAAAACGATTACTTATTTTAGAAGACGGCACTATTTTTGAAGGAGAACCCTTTGGTGCTGATATTGATGTGACTGG
TGAAATTGTCTTTAATACAGGAATGACAGGCTACCAAGAATCCATCACTGACCAATCCTACAATGGGCAAATTTTAACCT
TTACCTATCCTTTGATTGGAAATTATGGGATTAATCGAGATGACTATGAGTCCATTTCACCCACCTGTAAAGGGGTTGTT
GTGTCTGAAGTCAGTCGGTTGGCAAGTAATTGGCGCAAGCAAATGACGCTAGATGCGTTCTTGAAAATAAAAGGGATTCC
AGGGATTTCGGGAATTGATACGAGAGCTTTGACCAAAATTATTCGGCAACACGGTACCATGAAAGCTACGATGGCAGATG
ATGGAGATAGTATCCAGCATTTAAAAGATCAACTGCGTGCAACAGTCTTACCGACTAACACCATTGAACAAGTGTCAACT
AAAACAGCTTATCCTGCACCAGGGATCGGGAAAAATATTGTATTGGTTGATTTTGGTTTAAAACATTCTATTTTAAGAGA
ATTTTCTAAAAGACAATGTAATATCACCGTTGTCCCTTTCAACATCACTGCAGAAGAAGTGCTCCAACTAAATCCGGATG
GCTTGATGCTATCTAATGGCCCAGGAAACCCAGAAGATTTGCCAGAGGCTTTAGACATGATTCGTGGGGTACAAGGTAAG
ATTCCTATTTTTGGTATTTGTATGGGACATCAACTCTTTAGTCTAGCAAATGGTGCTAAGACTTGTAAAATGACCTTTGG
TCATCGTGGCTTTAACCATGCCGTTAGAGAAATTGCAACGGGAAGAATTGATTTTACCAGTCAAAACCATGGTTATGCCG
TTGAACGTAGTAGTCTACCTGATACTTTAATGGTTACGCATGAAGACATTAACGATAAGACGGTCGAAGGAGTGAAACAT
AGAGATTTTCCAGCTTTTTCAGTCCAATTTCACCCTGATGCGGCTCCAGGACCTCATGATGCCAGCTACTTATTTGACGA
ATTTTTGGAAATGATTGATTCTTGGAGATGTACGAGTAAATAG

Upstream 100 bases:

>100_bases
TTTGTTCGCATGGCTATTATTGAAGCCATTTTGAATGGTAGAAATAAAAACTCATGATGAAAGATTAAAGCATGTTTAGA
AGGTAGGAAAAGGTAGGAAA

Downstream 100 bases:

>100_bases
TAAATCAGTTGCAATTTCACGACTATATCACCTCTTGTTACTCTATTTATTAACATTTACAGTAATATTATTTAATGTTG
CTCTGTGAGGCAACAAATAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVV
VSEVSRLASNWRKQMTLDAFLKIKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGK
IPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKH
RDFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK

Sequences:

>Translated_360_residues
MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVV
VSEVSRLASNWRKQMTLDAFLKIKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGK
IPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKH
RDFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK
>Mature_359_residues
TKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVV
SEVSRLASNWRKQMTLDAFLKIKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVSTK
TAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGKI
PIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHR
DFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=365, Percent_Identity=41.3698630136986, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=37.5, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=37.5, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786215, Length=380, Percent_Identity=43.6842105263158, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1789760, Length=139, Percent_Identity=28.7769784172662, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=361, Percent_Identity=42.9362880886427, Blast_Score=258, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=381, Percent_Identity=40.4199475065617, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=37.6884422110553, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322638, Length=182, Percent_Identity=33.5164835164835, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45555749, Length=370, Percent_Identity=41.6216216216216, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24642586, Length=370, Percent_Identity=41.6216216216216, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_STRP1 (P63735)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269043.1
- RefSeq:   YP_282005.1
- ProteinModelPortal:   P63735
- SMR:   P63735
- EnsemblBacteria:   EBSTRT00000000874
- EnsemblBacteria:   EBSTRT00000027655
- GeneID:   3572271
- GeneID:   901000
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0833
- KEGG:   spz:M5005_Spy_0642
- GeneTree:   EBGT00050000027164
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   SPYO160490:SPY0833-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0642-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39758; Mature: 39626

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 247-247 ACT_SITE 331-331 ACT_SITE 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLIG
CCCEEEEEECCCEECCCCCCCCEEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEEEECC
NYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLKIKGIPGISGIDTRALTKI
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHH
IRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVSTKTAYPAPGIGKNIVLVDFGL
HHHCCCEEEEECCCCCHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCEEEEEECCH
KHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGK
HHHHHHHHHHCCCCEEEEEECCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHCCCCC
IPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLP
CCEEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCC
DTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK
CEEEEEECCCCCHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITDQSYNGQILTFTYPLIG
CCEEEEEECCCEECCCCCCCCEEEEEEEEEECCCCCHHHHHCCCCCCCEEEEEEEEECC
NYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLKIKGIPGISGIDTRALTKI
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHH
IRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVSTKTAYPAPGIGKNIVLVDFGL
HHHCCCEEEEECCCCCHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCEEEEEECCH
KHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLSNGPGNPEDLPEALDMIRGVQGK
HHHHHHHHHHCCCCEEEEEECCCHHHHHEECCCCEEEECCCCCHHHHHHHHHHHHCCCCC
IPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVREIATGRIDFTSQNHGYAVERSSLP
CCEEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCCEEEECCCCC
DTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDAAPGPHDASYLFDEFLEMIDSWRCTSK
CEEEEEECCCCCHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296