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The map label for this gene is ksgA

Identifier: 15674442

GI number: 15674442

Start: 226291

End: 227163

Strand: Direct

Name: ksgA

Synonym: SPy_0262

Alternate gene names: 15674442

Gene position: 226291-227163 (Clockwise)

Preceding gene: 15674441

Following gene: 15674443

Centisome position: 12.22

GC content: 42.61

Gene sequence:

>873_bases
ATGAGAATTGCAGATTATAGCGTGACCAAGGCTGTCCTTGATCGTCACGGATTTACCTTTAAGAAATCTTTTGGTCAGAA
CTTTTTGACCGATACCAATATTTTACAGAAAATCGTTGACACGGCTGAGATTGATCAGAATGTCAATGTTATCGAGATTG
GTCCTGGAATTGGGGCTTTGACGGAATTTTTGGCAGAAAATGCTGCTGAAGTGATGGCTTTTGAGATTGATGACCGCTTG
GTGCCGATTTTGGCAGATACCTTGCGCGATTTTGATAATGTGCAAGTGGTCAATCAAGATATTTTAAAAGCTGATTTGCA
GACCCAAATCAAGCAGTTTAAAAATCCTGATTTGCCCATTAAGGTGGTGGCTAATCTACCTTACTACATCACAACGCCTA
TTCTCATGCACTTGATTGAGAGCAAAATTCCATTCCAAGAGTTTGTGGTCATGATGCAGCGTGAAGTGGCAGATCGTATT
TCTGCGGAGCCAAACACTAAAGCTTATGGTTCCTTGTCCATTGCGGTGCAGTATTACATGACTGCCAAGGTTGCCTTCAT
TGTGCCGCGCACCGTCTTTGTACCTGCACCAAATGTCGATAGTGCTATCCTCAAAATGGTGCGTCGCGACCAGCCTTTGA
TTGAGGTTAAGGACGAAGATTTCTTTTTCAGAGTGTCTCGTCTCAGTTTTGTCCATCGTCGCAAGACCCTGTGGAATAAC
TTGACTAGCCATTTTGGGAAGTCTGAAGACATCAAGGCTAAACTTGAAAAAGGTCTGGCACTAGCAGATATCAAGCCTTC
TATTCGTGGTGAGGCCTTGTCGATTCAAGATTTTGGCAAATTAGCTGATGCTTTAAAAGAAGTTGGATTATAA

Upstream 100 bases:

>100_bases
ATATAACGGCCCCATTAACTGAATGGCGTGTCCTGGCAAAAAAGTTTAGGCTAGTGGTTCAAATGCCAAAAAGAAAGTGA
TCATAAAAAGAAGAGAATAT

Downstream 100 bases:

>100_bases
AAGCTTTCCCCCGGTGACAATAAAAAAATTCCCTTGTAGACAAAACAGAGATGTTCAGTTCAGGGGGATTTTTCTTATTG
AAGCTGGCCGTTAAGGCATC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEIDQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRL
VPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKDEDFFFRVSRLSFVHRRKTLWNN
LTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQDFGKLADALKEVGL

Sequences:

>Translated_290_residues
MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEIDQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRL
VPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKDEDFFFRVSRLSFVHRRKTLWNN
LTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQDFGKLADALKEVGL
>Mature_290_residues
MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEIDQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRL
VPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKDEDFFFRVSRLSFVHRRKTLWNN
LTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQDFGKLADALKEVGL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=287, Percent_Identity=30.3135888501742, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI7657198, Length=247, Percent_Identity=32.3886639676113, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786236, Length=276, Percent_Identity=31.8840579710145, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=27.8350515463918, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=230, Percent_Identity=31.304347826087, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=29.1479820627803, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=30.7017543859649, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357273, Length=310, Percent_Identity=26.7741935483871, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_STRPB (Q1JDL6)

Other databases:

- EMBL:   CP000261
- RefSeq:   YP_599836.1
- ProteinModelPortal:   Q1JDL6
- SMR:   Q1JDL6
- STRING:   Q1JDL6
- EnsemblBacteria:   EBSTRT00000043726
- GeneID:   4064865
- GenomeReviews:   CP000261_GR
- KEGG:   spj:MGAS2096_Spy0240
- eggNOG:   COG0030
- GeneTree:   EBGT00050000027760
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   SPYO370553:MGAS2096_SPY0240-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32702; Mature: 32702

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 35-35 BINDING 37-37 BINDING 62-62 BINDING 83-83 BINDING 108-108 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEIDQNVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
TEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPI
HHHHHHCHHHHEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRISAEPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKDEDFFFRVSRLSFVHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
LTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQDFGKLADALKEVGL
HHHHCCCCCHHHHHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEIDQNVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
TEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDILKADLQTQIKQFKNPDLPI
HHHHHHCHHHHEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRISAEPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKDEDFFFRVSRLSFVHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
LTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQDFGKLADALKEVGL
HHHHCCCCCHHHHHHHCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA