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The map label for this gene is leuS [H]

Identifier: 15674378

GI number: 15674378

Start: 157127

End: 159628

Strand: Direct

Name: leuS [H]

Synonym: SPy_0173

Alternate gene names: 15674378

Gene position: 157127-159628 (Clockwise)

Preceding gene: 15674377

Following gene: 15674379

Centisome position: 8.48

GC content: 46.16

Gene sequence:

>2502_bases
ATGACATTTTACGATCACACCGCTATTGAACCCAAATGGCAAGCCTTTTGGGCAGATAATCATACTTTTAAAACAGGAAC
AGACGCATCAAAACCTAAGTTTTATGCCCTAGATATGTTCCCATACCCATCTGGAGCTGGTCTCCACGTTGGTCACCCAG
AAGGCTATACAGCAACTGATATTCTCAGCCGTTTCAAACGTGCCCAAGGTCACAATATTCTCCACCCAATGGGTTGGGAT
GCCTTTGGACTTCCTGCTGAGCAGTACGCTATGGATACTGGTAATGACCCAGCCGAGTTTACAGCTGAAAACATCGCTAA
CTTCAAACGCCAGATTAATGCTCTTGGTTTCTCATACGACTGGGATCGTGAAGTTAACACAACTGACCCTAACTACTACA
AGTGGACACAGTGGATCTTTACCAAGCTTTATGAAAAAGGCCTAGCCTACGAAGCTGAAGTTCCCGTTAACTGGGTTGAA
GAATTGGGAACAGCTATCGCTAACGAAGAGGTTCTCCCTGATGGGACATCAGAACGTGGCGGCTACCCAGTTGTCAGAAA
ACCCATGCGCCAATGGATGCTCAAAATCACTGCCTATGCAGAGCGCTTGCTAGAAGACTTGGAAGAAGTTGACTGGCCAG
AATCAATCAAGGACATGCAACGCAACTGGATTGGTAAATCAACTGGTGCTAATGTAACCTTCAAGGTTAAGGATACAGAT
AAGGACTTCACTGTCTTTACAACACGTCCTGACACGCTTTTTGGTGCAACTTACGCCGTTCTTGCTCCTGAGCATGCCTT
GGTTGATGCTATTACAACAGCTGACCAAGCAGAAGCCGTTGCCAAATACAAACGTCAAGCAAGTCTCAAATCAGACCTTG
CTCGTACAGATCTTGCCAAAGAAAAAACTGGTGTGTGGACAGGTGCCTATGCGATTAACCCAGTTAATGGTAACGAAATG
CCAGTTTGGATTGCGGACTATGTCCTTGCCAGCTACGGTACTGGTGCTATCATGGCTGTTCCAGCCCACGATGAACGTGA
CTGGGAATTCGCAAAACAATTTAAGCTAGACATCATCCCTGTTCTTGAAGGTGGTAATGTTGAAGAGGCTGCCTTTACAG
AAGACGGTCTTCACATCAACTCAGGCTTCCTAGATGGTCTTGACAAAGCGTCAGCTATTGCCAAAATGGTCGAATGGCTT
GAAGCAGAAGGCGTCGGTAATGAGAAAGTCACTTACCGTCTTCGTGACTGGCTCTTTAGCCGTCAACGTTATTGGGGTGA
GCCAATCCCAATCATCCACTGGGAAGATGGGACCTCAACAGCGGTTCCTGAGAGTGAGTTGCCACTGGTTCTTCCAGTAA
CGAAGGATATCCGTCCTTCAGGTACTGGTGAGAGCCCACTTGCTAACGTGACAGACTGGTTGGAAGTGACTCGTGAAGAT
GGTGTGAAAGGTCGTCGTGAGACTAACACCATGCCACAATGGGCAGGTTCAAGCTGGTATTATCTTCGTTACATCGATCC
ACACAACACTGAAAAACTGGCTGACGAGGAACTCCTCAAACAATGGTTGCCAGTTGATATCTATGTCGGTGGTGCAGAGC
ATGCCGTGCTTCACCTTCTCTACGCTCGCTTCTGGCACAAGGTTCTTTACGATCTCGGTGTTGTACCTACCAAAGAGCCA
TTCCAAAAACTCTTTAACCAAGGGATGATTTTGGGGACAAGCTACCGCGATAGCCGTGGTGCTCTTGTGGCAACTGACAA
GGTTGAAAAACGTGATGGTAGCTTCTTCCATGTGGAAACTGGGGAAGAGCTTGAACAAGCACCAGCTAAGATGTCTAAAT
CACTTAAGAACGTTGTCAACCCAGATGATGTTGTTGAGCAGTACGGTGCTGACACTCTTCGTGTTTACGAGATGTTCATG
GGACCACTTGACGCTTCCATCGCCTGGTCTGAAGAAGGTCTTGAAGGCTCACGTAAGTTCTTGGACCGTGTTTACCGTTT
GATCACAACTAAAGAGATCACTGAGGAAAATAGCGGAGCCCTTGACAAGGTTTACAACGAAACCGTTAAGGCCGTTACTG
AGCAAGTTGACCAAATGAAATTCAACACAGCTATCGCTCAACTCATGGTATTTGTCAATGCAGCCAACAAGGAAGACAAA
CTCTTTAGCGACTATGCCAAAGGCTTTGTTCAATTGATTGCACCATTTGCACCGCACTTAGGTGAGGAACTCTGGCAAGC
CTTGACAGCCTCAGGTGAGTCAATCTCTTACGTGCCATGGCCAAGTTATGATGAAAGCAAATTGGTTGAAAACGATGTGG
AAATCGTTGTCCAAATCAAAGGTAAGGTCAAAGCTAAACTTGTAGTTGCTAAAGACCTTTCACGCGAAGAATTACAAGAA
GTTGCTCTTGCTAACGAAAAAGTCCAAGCCGAAATCGCAGGCAAAGACATCATCAAAGTCATCGCAGTACCAAACAAACT
CGTTAATATTGTTATCAAATAG

Upstream 100 bases:

>100_bases
AGTCTCAAAAGCCAAGGTGCTAGTCATGTCTTGACCATTCCTAAAAGCTTCATCTCATGCTATAATAAGAAGACTAGATT
TTTTACATGGGAGATTTCAC

Downstream 100 bases:

>100_bases
TTTGAGGTGACACAGCCCTTCAGTGGTTATTTGCGTCACGTCACAGTTAATGCCAAAGAGCTGATCTATTCAAAAAGCAA
CTTATCTAGAAAAACCACAA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNILHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAKYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGNEM
PVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASAIAKMVEWL
EAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQE
VALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK

Sequences:

>Translated_833_residues
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNILHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAKYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGNEM
PVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASAIAKMVEWL
EAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEP
FQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQE
VALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
>Mature_832_residues
TFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRFKRAQGHNILHPMGWDA
FGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEE
LGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTDK
DFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAKYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGNEMP
VWIADYVLASYGTGAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASAIAKMVEWLE
AEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTREDG
VKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPF
QKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMG
PLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKL
FSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREELQEV
ALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=858, Percent_Identity=34.1491841491841, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI94721239, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI94721241, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786861, Length=865, Percent_Identity=42.4277456647399, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=387, Percent_Identity=26.0981912144703, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71997510, Length=775, Percent_Identity=29.4193548387097, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=29.4193548387097, Blast_Score=326, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=29.0322580645161, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17541896, Length=923, Percent_Identity=21.2351029252438, Blast_Score=91, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323414, Length=854, Percent_Identity=34.7775175644028, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6321531, Length=433, Percent_Identity=24.4803695150115, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355409, Length=837, Percent_Identity=32.3775388291517, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281366294, Length=385, Percent_Identity=25.1948051948052, Blast_Score=67, Evalue=7e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93817; Mature: 93685

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCCCEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGHNILHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAKYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGNEMPVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFKLDIIP
HHCCCEECCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEE
VLEGGNVEEAAFTEDGLHINSGFLDGLDKASAIAKMVEWLEAEGVGNEKVTYRLRDWLFS
EECCCCCCHHHCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCEEEEEEEC
GKVKAKLVVAKDLSREELQEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
CCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHCCCHHEEEEECCCHHEEEECC
>Mature Secondary Structure 
TFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCCEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRFKRAQGHNILHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGANVTFKVKDTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
KDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAKYKRQASLKSDLARTDLAK
CCEEEEECCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGNEMPVWIADYVLASYGTGAIMAVPAHDERDWEFAKQFKLDIIP
HHCCCEECCEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCEEEE
VLEGGNVEEAAFTEDGLHINSGFLDGLDKASAIAKMVEWLEAEGVGNEKVTYRLRDWLFS
EECCCCCCHHHCCCCCCEECCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANVTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
YARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDSRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITTKEITEENSGALDKVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LFSDYAKGFVQLIAPFAPHLGEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCEEEEEEEC
GKVKAKLVVAKDLSREELQEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK
CCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHCCCHHEEEEECCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA