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The map label for this gene is metB [H]

Identifier: 15674377

GI number: 15674377

Start: 155813

End: 156952

Strand: Direct

Name: metB [H]

Synonym: SPy_0172

Alternate gene names: 15674377

Gene position: 155813-156952 (Clockwise)

Preceding gene: 15674373

Following gene: 15674378

Centisome position: 8.41

GC content: 42.11

Gene sequence:

>1140_bases
ATGAGAGAAAATACGACCTTATTACATGGCTATACGGTTATTGATGAGTTTACAGGAGCAGCTTCAGTTCCCATCTATCA
GACTTCGACGTTCCACAATTCAGAACTGTATTGTCCTAGTCAAAAACATTTGTACACACGCTTTTCAAACCCAACGACTG
AAGCTTTAGAAGACGGGTTAGCCTGTTTAGAAAAAGCGACTTATGCGGTGGCTTATGCATCTGGTATGGCTGCTATTTCA
ACTGTTTTGATGCTCTTAAAAGCTGGAGATCATGTGATTTTCCCATTGGAGGTCTATGGAGGGACTTGCCAATTTGCGAC
AGCTATTTTACCCAATTATCAGATTGAAACAAGCTTTGTAGATATGGCTGATTTAGCAACAGTAAAAGCAAGTATCAGGC
CTAATACGCGTATGATTTATCTAGAGACACCGTCAAATCCGCTCTTGAAAATTTGTGATATTTCAGAGCTTGTGCAACTC
GCCAAAGCTTATGGTGTGCTAACTGTGGCTGATAATACCTTTATGACAAGTTTGTATCAAGAACCCTTAGCTATGGGTGT
GGATATTGTGGTAGAAAGTGTGACCAAATTTATCAATGGTCATAGTGATGTGGTAGCTGGTTTAGCTGCTACCAATAATG
AGGCTATCTACAACCAGCTCAAGCTTTTTCAAAAAAACTTTGGAGCAATTGTTGGGGTCGAAGACGCTTGGCTGATTCTT
CGTGGCATGAAAACTATGGGGATCCGTATGGAACAAGCGGTAAAAAATGCTCAGCAGCTCGCTAACTATTTAGCGAAGCA
CCCCAAAGTTCTTAAGGTACATTACCCTGGCTTAGACAGTCATCCTAATCACGACACCCACCTGCAACAAGCTAAGAATG
GTGGTGCTGTTTTATCCTTTGAGTTGGCTTCTAAGGAGGAGCTGATGACCTTTACCCATCGTATTCAGTTGCCGATTTTA
GCCGTTAGTCTAGGTGGAGTAGAGTCCATTTTGTCACATCCAGCAACGATGTCTCATGCTTGTTTATCACCACAAGCGCG
GTTGGAACAAGGGGTTGTTGATGGTTTGCTGCGCTTGTCTTGTGGTGTTGAAAATATCGAAGACTTATTAGCCGATTTTG
AGCAAGCACTTGCTTTTTAA

Upstream 100 bases:

>100_bases
CGCTTTTTTGAAGAGCTTTTTGGTTTGTGCAACCATTGGGGTGGGACCATTGTCTAAAAGAGAGTTCATTGGAGTTAACG
CTATTGCGGAGGTGTACGAA

Downstream 100 bases:

>100_bases
GTCTGGCTTTTTTATTAGCTTTTTGTGAGGATGGTTGATGCTAAAGCAGGGTTTAAAAATAGCTAATCGTCTGAAGTCTC
AAAAGCCAAGGTGCTAGTCA

Product: putative cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACLEKATYAVAYASGMAAIS
TVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQKNFGAIVGVEDAWLIL
RGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPIL
AVSLGGVESILSHPATMSHACLSPQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF

Sequences:

>Translated_379_residues
MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACLEKATYAVAYASGMAAIS
TVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQKNFGAIVGVEDAWLIL
RGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPIL
AVSLGGVESILSHPATMSHACLSPQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF
>Mature_379_residues
MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGLACLEKATYAVAYASGMAAIS
TVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQKNFGAIVGVEDAWLIL
RGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPIL
AVSLGGVESILSHPATMSHACLSPQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF

Specific function: Catalyzes the transformation of cystathionine into homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=368, Percent_Identity=39.945652173913, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI299473759, Length=368, Percent_Identity=35.054347826087, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI25453487, Length=367, Percent_Identity=35.6948228882834, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1790375, Length=365, Percent_Identity=39.4520547945205, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1789383, Length=359, Percent_Identity=27.2980501392758, Blast_Score=158, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17538055, Length=358, Percent_Identity=40.5027932960894, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17560056, Length=360, Percent_Identity=40.8333333333333, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71980708, Length=365, Percent_Identity=37.8082191780822, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=369, Percent_Identity=41.4634146341463, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6321254, Length=387, Percent_Identity=33.8501291989664, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=318, Percent_Identity=31.7610062893082, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=414, Percent_Identity=28.2608695652174, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322590, Length=255, Percent_Identity=28.2352941176471, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=268, Percent_Identity=27.6119402985075, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323557, Length=358, Percent_Identity=24.5810055865922, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=38.6243386243386, Blast_Score=274, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41385; Mature: 41385

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGL
CCCCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCEEECCCHHHHEEECCCCHHHHHHHHH
ACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCEEEEHHH
DMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQLAKAYGVLTVADNTFMTSLYQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCEEEEECCHHHHHHHH
EPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQKNFGAIVGVEDAWLIL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCHHHHHH
RGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSF
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
ELASKEELMTFTHRIQLPILAVSLGGVESILSHPATMSHACLSPQARLEQGVVDGLLRLS
ECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
CGVENIEDLLADFEQALAF
CCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHLYTRFSNPTTEALEDGL
CCCCCEEEEEEEHHHHCCCCCCCCEEEECCCCCCEEECCCHHHHEEECCCCHHHHHHHHH
ACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGTCQFATAILPNYQIETSFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCEEEEHHH
DMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQLAKAYGVLTVADNTFMTSLYQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCEEEEECCHHHHHHHH
EPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYNQLKLFQKNFGAIVGVEDAWLIL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCHHHHHH
RGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYPGLDSHPNHDTHLQQAKNGGAVLSF
HHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
ELASKEELMTFTHRIQLPILAVSLGGVESILSHPATMSHACLSPQARLEQGVVDGLLRLS
ECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
CGVENIEDLLADFEQALAF
CCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]