Click here to switch to the map view.

The map label for this gene is asnO [H]

Identifier: 13474904

GI number: 13474904

Start: 4756027

End: 4757919

Strand: Direct

Name: asnO [H]

Synonym: mlr5888

Alternate gene names: 13474904

Gene position: 4756027-4757919 (Clockwise)

Preceding gene: 13474903

Following gene: 13474906

Centisome position: 67.59

GC content: 54.99

Gene sequence:

>1893_bases
ATGTGCGGAATCTTTGGGATAGTCCTCAAAAACGAGGGAACACCCGTTTGTGCTGCGGCCGTTGAGCGTTGCGCTGACGC
TCTCGCTCATCGCGGACCTGATGCCAGCGGCCTATACATTAATCAATCGGTAGCATTCGCGCATCGACGGCTATCGATTG
TCGACGCTCATTCTCGAGCCAATCAGCCCTATCGCGATGCCAATACCGGAGTGGTGGTAACGTACAACGGCGAGATATTC
AACTATCGGGAAATTAGAACTGAATTGAGGGATATGGGTTTTTTCTTCTCGACGGTGTCCGACACCGAGGTTCTAATTCT
TAGCTATATTGCTTGGGGAAAGTCATTCTTAAATCGTCTTGACGGGATATTTGCCTTCGCGCTTTTCGACCCGCGGAACT
CGCTTGTTCTCATCGCCCGAGATCGTTTGGGGGTAAAACCCCTTTACTACACGATCTGCGACGTTGGGCTTCTGTTTGCG
TCGCAGCCGAACGCTCTCATTCGCTGGCCGGGCGTCATGCGCGGACCCGACATGATCGGCGCGCTCAGCTTTCTATCCTA
TCGCGCGGTTGTCGGCGCCAGAACGCTGTTCGCCGGGATTTCCAAGTTAGAGCCCGGCCATCTCCTCGAAATAAGAAATG
GGCGACACACGTCCGAACGTTGGTGGAATCTGTCAGAGCGCATTTCGCGATGTAAGCCCGACTACTCCGACATACGCAGA
CTACTCGGAAGCGCCGTCGAACGTCAGCTGGTGGCTGACGTACCGGTCGCCGCATTGTTGTCCGGTGGACTTGACTCGAG
CATCCTCGCCTATGAGGCGTCCGCCCGCTCCCAAATCCGTCCGATCTGCTATACCGGCAAGGTCGAGACCGCCGATTATG
ACGAGACGGCTTATGCGATGGAGGTCGCCGCAGAATTCGGGCTCACTCACCGGGTCGTCCCAATTGGAGAACCTTCCGAT
ATCTACGCTGTGTCAGAGCTGGTTCGCCTGCGCGGACATCCGCTTGGGATGCATAACGAAATCGCAATGTTTACGCTCGC
AAAGGCGATTTCGAAGGAGCATAAGGTCGTCCTCACCGGCGAGGGGGCCGATGAGATTTTTGCTGGCTACAGCCGATTGT
TCCGTACGCCGTATGATTACAATCGAAGCAAGATCATCGCCGCATTACCACGTGCTCTTGCCCAGGTCGCACGCCGGCGC
CTCGGACTCGACGTCCATGGCAGTCAATTGCAGTTCTTCCTGGCGCGTTACAGCTACTTCCCGACCGATGAAATCCGCTC
GCTTGTCATCGATGGGCGGGTACGAAGGGAGCACGAGATGGCTCTGTTCGATCACTTCTCGACTCAATTTGCAGAGGCCG
GCGGAGATTTCTTTAGCAAGATCTCATATGTTCTCGTCTCTACGCATTTGCCCGCATTGCTGGAAATGGTTGACAACACC
ACAATGGCAGCTGGGCTTGAAGCTCGCGTTCCTTATACCGATCACCAGCTCGTTACCGCCGCAATGGCGATGCCCGGCTC
ACAGCGGTTGAGGTGGAGGTCTCCAATCGCTTCGGTCCAGGCACTGTGGCATCCGGTTGCTTCCTTCAGCGAACGTCTCG
ATGTCTCAAAATTCCCCTTAAGAAGGGAATACCGGAGCGTCTTGCCCAGATCTGTTCTATCTCGCAAAAAGATGGGATTT
CCGCTGCCACTGGGTCAGTGGGCCGTTGGCGAGGGAGCAACGGAATATCGAAGGCTGCTGTTTGATAAGGACTCTCCACT
GGGAGATTTGTTCGATCCTGCTGCTTTGCGGCGTTGGTTCGAGGCCGGACGACGAAATCCTTCAGACTCGTTTGGTCGGA
AGTTCTGGCTGTTGGCCAACCTTGGGATATTCTTCAAAGAGGTATTCTCATGA

Upstream 100 bases:

>100_bases
ACCCGCTGGCCCGCTTCTGGTTCGGCGATGGCTGCATCCAGATGATCGGCGAAAAGCCGAGCACGTAGTCGGCGTTATTC
GTGCCAAGAGAGCGACCCCA

Downstream 100 bases:

>100_bases
CGAATATGCGAGCCCGCAGGCGGTCGCTTGCGGGCATGTTACAGAAGACCCCTTGCCGAAAACGGGGCGTCGGACAATCC
CAAGATCTGAGCAATGGTTG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MCGIFGIVLKNEGTPVCAAAVERCADALAHRGPDASGLYINQSVAFAHRRLSIVDAHSRANQPYRDANTGVVVTYNGEIF
NYREIRTELRDMGFFFSTVSDTEVLILSYIAWGKSFLNRLDGIFAFALFDPRNSLVLIARDRLGVKPLYYTICDVGLLFA
SQPNALIRWPGVMRGPDMIGALSFLSYRAVVGARTLFAGISKLEPGHLLEIRNGRHTSERWWNLSERISRCKPDYSDIRR
LLGSAVERQLVADVPVAALLSGGLDSSILAYEASARSQIRPICYTGKVETADYDETAYAMEVAAEFGLTHRVVPIGEPSD
IYAVSELVRLRGHPLGMHNEIAMFTLAKAISKEHKVVLTGEGADEIFAGYSRLFRTPYDYNRSKIIAALPRALAQVARRR
LGLDVHGSQLQFFLARYSYFPTDEIRSLVIDGRVRREHEMALFDHFSTQFAEAGGDFFSKISYVLVSTHLPALLEMVDNT
TMAAGLEARVPYTDHQLVTAAMAMPGSQRLRWRSPIASVQALWHPVASFSERLDVSKFPLRREYRSVLPRSVLSRKKMGF
PLPLGQWAVGEGATEYRRLLFDKDSPLGDLFDPAALRRWFEAGRRNPSDSFGRKFWLLANLGIFFKEVFS

Sequences:

>Translated_630_residues
MCGIFGIVLKNEGTPVCAAAVERCADALAHRGPDASGLYINQSVAFAHRRLSIVDAHSRANQPYRDANTGVVVTYNGEIF
NYREIRTELRDMGFFFSTVSDTEVLILSYIAWGKSFLNRLDGIFAFALFDPRNSLVLIARDRLGVKPLYYTICDVGLLFA
SQPNALIRWPGVMRGPDMIGALSFLSYRAVVGARTLFAGISKLEPGHLLEIRNGRHTSERWWNLSERISRCKPDYSDIRR
LLGSAVERQLVADVPVAALLSGGLDSSILAYEASARSQIRPICYTGKVETADYDETAYAMEVAAEFGLTHRVVPIGEPSD
IYAVSELVRLRGHPLGMHNEIAMFTLAKAISKEHKVVLTGEGADEIFAGYSRLFRTPYDYNRSKIIAALPRALAQVARRR
LGLDVHGSQLQFFLARYSYFPTDEIRSLVIDGRVRREHEMALFDHFSTQFAEAGGDFFSKISYVLVSTHLPALLEMVDNT
TMAAGLEARVPYTDHQLVTAAMAMPGSQRLRWRSPIASVQALWHPVASFSERLDVSKFPLRREYRSVLPRSVLSRKKMGF
PLPLGQWAVGEGATEYRRLLFDKDSPLGDLFDPAALRRWFEAGRRNPSDSFGRKFWLLANLGIFFKEVFS
>Mature_630_residues
MCGIFGIVLKNEGTPVCAAAVERCADALAHRGPDASGLYINQSVAFAHRRLSIVDAHSRANQPYRDANTGVVVTYNGEIF
NYREIRTELRDMGFFFSTVSDTEVLILSYIAWGKSFLNRLDGIFAFALFDPRNSLVLIARDRLGVKPLYYTICDVGLLFA
SQPNALIRWPGVMRGPDMIGALSFLSYRAVVGARTLFAGISKLEPGHLLEIRNGRHTSERWWNLSERISRCKPDYSDIRR
LLGSAVERQLVADVPVAALLSGGLDSSILAYEASARSQIRPICYTGKVETADYDETAYAMEVAAEFGLTHRVVPIGEPSD
IYAVSELVRLRGHPLGMHNEIAMFTLAKAISKEHKVVLTGEGADEIFAGYSRLFRTPYDYNRSKIIAALPRALAQVARRR
LGLDVHGSQLQFFLARYSYFPTDEIRSLVIDGRVRREHEMALFDHFSTQFAEAGGDFFSKISYVLVSTHLPALLEMVDNT
TMAAGLEARVPYTDHQLVTAAMAMPGSQRLRWRSPIASVQALWHPVASFSERLDVSKFPLRREYRSVLPRSVLSRKKMGF
PLPLGQWAVGEGATEYRRLLFDKDSPLGDLFDPAALRRWFEAGRRNPSDSFGRKFWLLANLGIFFKEVFS

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=412, Percent_Identity=26.2135922330097, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI168229252, Length=412, Percent_Identity=26.2135922330097, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI168229250, Length=412, Percent_Identity=26.2135922330097, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786889, Length=393, Percent_Identity=31.5521628498728, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71993933, Length=398, Percent_Identity=30.4020100502513, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25147557, Length=398, Percent_Identity=30.4020100502513, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=322, Percent_Identity=30.1242236024845, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325403, Length=423, Percent_Identity=29.0780141843972, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321563, Length=415, Percent_Identity=28.1927710843373, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45553209, Length=405, Percent_Identity=28.8888888888889, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70586; Mature: 70586

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGIVLKNEGTPVCAAAVERCADALAHRGPDASGLYINQSVAFAHRRLSIVDAHSRA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHEEECHHHC
NQPYRDANTGVVVTYNGEIFNYREIRTELRDMGFFFSTVSDTEVLILSYIAWGKSFLNRL
CCCCCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DGIFAFALFDPRNSLVLIARDRLGVKPLYYTICDVGLLFASQPNALIRWPGVMRGPDMIG
HHHEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHH
ALSFLSYRAVVGARTLFAGISKLEPGHLLEIRNGRHTSERWWNLSERISRCKPDYSDIRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCHHHHHHHCCCCHHHHHH
LLGSAVERQLVADVPVAALLSGGLDSSILAYEASARSQIRPICYTGKVETADYDETAYAM
HHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEECCHHCCCCCEEEECCCCCCCCCHHHHHH
EVAAEFGLTHRVVPIGEPSDIYAVSELVRLRGHPLGMHNEIAMFTLAKAISKEHKVVLTG
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEC
EGADEIFAGYSRLFRTPYDYNRSKIIAALPRALAQVARRRLGLDVHGSQLQFFLARYSYF
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PTDEIRSLVIDGRVRREHEMALFDHFSTQFAEAGGDFFSKISYVLVSTHLPALLEMVDNT
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TMAAGLEARVPYTDHQLVTAAMAMPGSQRLRWRSPIASVQALWHPVASFSERLDVSKFPL
HHHCCCEECCCCCCHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCH
RREYRSVLPRSVLSRKKMGFPLPLGQWAVGEGATEYRRLLFDKDSPLGDLFDPAALRRWF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EAGRRNPSDSFGRKFWLLANLGIFFKEVFS
HHHCCCCCHHHCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIFGIVLKNEGTPVCAAAVERCADALAHRGPDASGLYINQSVAFAHRRLSIVDAHSRA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHEEECHHHC
NQPYRDANTGVVVTYNGEIFNYREIRTELRDMGFFFSTVSDTEVLILSYIAWGKSFLNRL
CCCCCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DGIFAFALFDPRNSLVLIARDRLGVKPLYYTICDVGLLFASQPNALIRWPGVMRGPDMIG
HHHEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHH
ALSFLSYRAVVGARTLFAGISKLEPGHLLEIRNGRHTSERWWNLSERISRCKPDYSDIRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCHHHHHHHCCCCHHHHHH
LLGSAVERQLVADVPVAALLSGGLDSSILAYEASARSQIRPICYTGKVETADYDETAYAM
HHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEECCHHCCCCCEEEECCCCCCCCCHHHHHH
EVAAEFGLTHRVVPIGEPSDIYAVSELVRLRGHPLGMHNEIAMFTLAKAISKEHKVVLTG
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEC
EGADEIFAGYSRLFRTPYDYNRSKIIAALPRALAQVARRRLGLDVHGSQLQFFLARYSYF
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PTDEIRSLVIDGRVRREHEMALFDHFSTQFAEAGGDFFSKISYVLVSTHLPALLEMVDNT
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
TMAAGLEARVPYTDHQLVTAAMAMPGSQRLRWRSPIASVQALWHPVASFSERLDVSKFPL
HHHCCCEECCCCCCHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCH
RREYRSVLPRSVLSRKKMGFPLPLGQWAVGEGATEYRRLLFDKDSPLGDLFDPAALRRWF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EAGRRNPSDSFGRKFWLLANLGIFFKEVFS
HHHCCCCCHHHCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]