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The map label for this gene is lip2 [H]

Identifier: 13473786

GI number: 13473786

Start: 3576948

End: 3577907

Strand: Reverse

Name: lip2 [H]

Synonym: mll4497

Alternate gene names: 13473786

Gene position: 3577907-3576948 (Counterclockwise)

Preceding gene: 13473787

Following gene: 13473780

Centisome position: 50.85

GC content: 68.44

Gene sequence:

>960_bases
ATGACAGCAGACCGACACCACGGCAAATATGAAACACGGCTCGACCCGGCGCTGTGGGCCTATATCGACAGGGTCGACGC
CTGGTACCCGCCCGAGGTGATCGGCCTGCCGATCGACAAGCAGCGCGCGGTCTATGACAGAATGTGCCGGGCTTTTCACC
AAGGCCGCCCGCCCGGGGTCAAGGCCAGCGACGGCCTGGTTGCCGCCCCAGGCCACGGTATACCGATCCGCCACTACCGG
CCCGCCGGCAAGGCGGCGCGGGCCATGGTGCTCTATTTCCATGGCGGCGGCTTCATTCTGGGTAACCTCGACAGCCATGA
CGACATCTGCGCCGAGATATGCGCCGGCACCGGCTTCGACGTGCTGTCGGTCGACTACCGGCTGGCGCCTGAGCATGTCC
ACCCCGCCGCCTTCGACGATGCGATGGCCGCCTTCGCATGGGCGGCGGAGACGTCACGCCTGCCGCTGGTGCTGTGCGGC
GAGAGCGCCGGCGGCAATCTGGCGGCGGCAGTGGCGCAAGCGACTCGCAAGCACGCGCGTACGGCGGTCGGCCAGGTGCT
GATCTATCCCGGCCTCGGCGGCGACGAGGGCGCCGGCTCCTATGTCGAACATGCCGAGGCGCCACTGCTGTCCGTCGGCG
ATATCGCCTTCTACCGCGACGTCCGTTCAGCCAAGCGGCAGTCGCCCGACGACCCGACCTTTTCACCGCTGCGCGACCGC
CACTTTTCCGGCCTGCCGCCAACGGTGATCATCACGGCCGAGTGCGACCCGCTGTCGTCGGATGGCGAGACCTACCGCGA
CCGCATCGCTGCGGCCGGCGGCAAGGCATGGTGGCACGAGGAGAAGCGGCTGGTGCACAGTTTCCTGCGCGCCCGCACCA
CGGTGCCGGCAGCGGCGGAAGCTTTTAGCCGCATCATCGCGGCGATCGCGGCGCTTGGCCGGGGTGAGTGGCCGTATTGA

Upstream 100 bases:

>100_bases
GCCGGCTGCTGGCCGGCGAGACGGTCACCGCCTGGCAGGTCGAGGGGTCGGTGCCGCTGCGCTTCACCTTCGACAATGCG
GCGGCGCTCTACGCCGAGCT

Downstream 100 bases:

>100_bases
GTCTTACGACGCCGCGATGCTCTTTTCGGAGGAGTTGGCTTTCAGCCAGAGCGCCTTTTCGCCGAGCGTGGCGACCATGG
CGGCATGCGCGGCGCGCTCC

Product: lipase (esterase)

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTADRHHGKYETRLDPALWAYIDRVDAWYPPEVIGLPIDKQRAVYDRMCRAFHQGRPPGVKASDGLVAAPGHGIPIRHYR
PAGKAARAMVLYFHGGGFILGNLDSHDDICAEICAGTGFDVLSVDYRLAPEHVHPAAFDDAMAAFAWAAETSRLPLVLCG
ESAGGNLAAAVAQATRKHARTAVGQVLIYPGLGGDEGAGSYVEHAEAPLLSVGDIAFYRDVRSAKRQSPDDPTFSPLRDR
HFSGLPPTVIITAECDPLSSDGETYRDRIAAAGGKAWWHEEKRLVHSFLRARTTVPAAAEAFSRIIAAIAALGRGEWPY

Sequences:

>Translated_319_residues
MTADRHHGKYETRLDPALWAYIDRVDAWYPPEVIGLPIDKQRAVYDRMCRAFHQGRPPGVKASDGLVAAPGHGIPIRHYR
PAGKAARAMVLYFHGGGFILGNLDSHDDICAEICAGTGFDVLSVDYRLAPEHVHPAAFDDAMAAFAWAAETSRLPLVLCG
ESAGGNLAAAVAQATRKHARTAVGQVLIYPGLGGDEGAGSYVEHAEAPLLSVGDIAFYRDVRSAKRQSPDDPTFSPLRDR
HFSGLPPTVIITAECDPLSSDGETYRDRIAAAGGKAWWHEEKRLVHSFLRARTTVPAAAEAFSRIIAAIAALGRGEWPY
>Mature_318_residues
TADRHHGKYETRLDPALWAYIDRVDAWYPPEVIGLPIDKQRAVYDRMCRAFHQGRPPGVKASDGLVAAPGHGIPIRHYRP
AGKAARAMVLYFHGGGFILGNLDSHDDICAEICAGTGFDVLSVDYRLAPEHVHPAAFDDAMAAFAWAAETSRLPLVLCGE
SAGGNLAAAVAQATRKHARTAVGQVLIYPGLGGDEGAGSYVEHAEAPLLSVGDIAFYRDVRSAKRQSPDDPTFSPLRDRH
FSGLPPTVIITAECDPLSSDGETYRDRIAAAGGKAWWHEEKRLVHSFLRARTTVPAAAEAFSRIIAAIAALGRGEWPY

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=135, Percent_Identity=41.4814814814815, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157041239, Length=181, Percent_Identity=30.3867403314917, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI61966717, Length=151, Percent_Identity=35.0993377483444, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1786682, Length=274, Percent_Identity=27.3722627737226, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=133, Percent_Identity=38.3458646616541, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=136, Percent_Identity=36.7647058823529, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=110, Percent_Identity=38.1818181818182, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=135, Percent_Identity=34.0740740740741, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34501; Mature: 34370

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADRHHGKYETRLDPALWAYIDRVDAWYPPEVIGLPIDKQRAVYDRMCRAFHQGRPPGV
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCC
KASDGLVAAPGHGIPIRHYRPAGKAARAMVLYFHGGGFILGNLDSHDDICAEICAGTGFD
CCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCC
VLSVDYRLAPEHVHPAAFDDAMAAFAWAAETSRLPLVLCGESAGGNLAAAVAQATRKHAR
EEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
TAVGQVLIYPGLGGDEGAGSYVEHAEAPLLSVGDIAFYRDVRSAKRQSPDDPTFSPLRDR
HHHCCEEEECCCCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
HFSGLPPTVIITAECDPLSSDGETYRDRIAAAGGKAWWHEEKRLVHSFLRARTTVPAAAE
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
AFSRIIAAIAALGRGEWPY
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TADRHHGKYETRLDPALWAYIDRVDAWYPPEVIGLPIDKQRAVYDRMCRAFHQGRPPGV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCC
KASDGLVAAPGHGIPIRHYRPAGKAARAMVLYFHGGGFILGNLDSHDDICAEICAGTGFD
CCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCC
VLSVDYRLAPEHVHPAAFDDAMAAFAWAAETSRLPLVLCGESAGGNLAAAVAQATRKHAR
EEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
TAVGQVLIYPGLGGDEGAGSYVEHAEAPLLSVGDIAFYRDVRSAKRQSPDDPTFSPLRDR
HHHCCEEEECCCCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHC
HFSGLPPTVIITAECDPLSSDGETYRDRIAAAGGKAWWHEEKRLVHSFLRARTTVPAAAE
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
AFSRIIAAIAALGRGEWPY
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]