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The map label for this gene is entB [C]

Identifier: 13473787

GI number: 13473787

Start: 3577904

End: 3578680

Strand: Reverse

Name: entB [C]

Synonym: mll4498

Alternate gene names: 13473787

Gene position: 3578680-3577904 (Counterclockwise)

Preceding gene: 13473788

Following gene: 13473786

Centisome position: 50.86

GC content: 66.92

Gene sequence:

>777_bases
GTGGATGACGAAGGTGGGGAGGCACCGGCCAATCACCACCGTTTGCGCCGTTGCGCTCACCGGAACGATGCTGACCGCAA
ACCCAGGCCGCACCGCATGAAAACCCCCCTCTCCATTAAGCGCGGTACGGTGGCCGCCGTGTTCATAGACCTGCAGGAAG
AGCACCGGCAGGACAAGCGCTACCTCGTCGAGGGCTTTGCCGGCATCCTCGCCAATGTCCAGCGGCTGCAAGAAGCGGCG
CGCGCCAATCACGTGCCGCTCCAGCACTGGGCCTATATCGTCGATCTCGACAAACAGGATCGGCCCTTTCATCCGCTCGG
TGCCGACGGCAAGTCGGCCTTCTCAGACAAGAGCGATCCGCTGACCGAGATCTGCCATGAGGTGGCGCCGGCCAGGGACG
AAGCGCTGCTGGTCAAGGCCGAGGCCAGCGCCTTCCGCAGCGGGCCGGCGGCTGATCAGCTCAAGGCTGCCGGTATCGAA
TGGCTGGTCGTCGCCGGCGTCTGGACCGAGGCCTGCATCGACGCCACCGTGAAGGACGCCGTGGCAAGGGGATTTCGCGT
GCTGCTGGTCAAGGATGCCTGCGGCAGCGGCAGCGCGGCCATGCACCAGACCGGCATCCTCAACCTCGCCAACCGGCTCT
ATGGCGGCGCGGTCACCGACACGGACGGCGCCTGCCGGCTGCTGGCCGGCGAGACGGTCACCGCCTGGCAGGTCGAGGGG
TCGGTGCCGCTGCGCTTCACCTTCGACAATGCGGCGGCGCTCTACGCCGAGCTATGA

Upstream 100 bases:

>100_bases
GCGTCGCCACGATCCAGAATTCTGCTCCGCTGCGCCCGTCAGAAAGGTCACGGCATGGATCCTCGGGTCTCCGCGACGGA
GCTTCGCTCCTGCTCCGCCC

Downstream 100 bases:

>100_bases
CAGCAGACCGACACCACGGCAAATATGAAACACGGCTCGACCCGGCGCTGTGGGCCTATATCGACAGGGTCGACGCCTGG
TACCCGCCCGAGGTGATCGG

Product: hypothetical protein

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: Isochorismatase

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MDDEGGEAPANHHRLRRCAHRNDADRKPRPHRMKTPLSIKRGTVAAVFIDLQEEHRQDKRYLVEGFAGILANVQRLQEAA
RANHVPLQHWAYIVDLDKQDRPFHPLGADGKSAFSDKSDPLTEICHEVAPARDEALLVKAEASAFRSGPAADQLKAAGIE
WLVVAGVWTEACIDATVKDAVARGFRVLLVKDACGSGSAAMHQTGILNLANRLYGGAVTDTDGACRLLAGETVTAWQVEG
SVPLRFTFDNAAALYAEL

Sequences:

>Translated_258_residues
MDDEGGEAPANHHRLRRCAHRNDADRKPRPHRMKTPLSIKRGTVAAVFIDLQEEHRQDKRYLVEGFAGILANVQRLQEAA
RANHVPLQHWAYIVDLDKQDRPFHPLGADGKSAFSDKSDPLTEICHEVAPARDEALLVKAEASAFRSGPAADQLKAAGIE
WLVVAGVWTEACIDATVKDAVARGFRVLLVKDACGSGSAAMHQTGILNLANRLYGGAVTDTDGACRLLAGETVTAWQVEG
SVPLRFTFDNAAALYAEL
>Mature_258_residues
MDDEGGEAPANHHRLRRCAHRNDADRKPRPHRMKTPLSIKRGTVAAVFIDLQEEHRQDKRYLVEGFAGILANVQRLQEAA
RANHVPLQHWAYIVDLDKQDRPFHPLGADGKSAFSDKSDPLTEICHEVAPARDEALLVKAEASAFRSGPAADQLKAAGIE
WLVVAGVWTEACIDATVKDAVARGFRVLLVKDACGSGSAAMHQTGILNLANRLYGGAVTDTDGACRLLAGETVTAWQVEG
SVPLRFTFDNAAALYAEL

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 28065; Mature: 28065

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDEGGEAPANHHRLRRCAHRNDADRKPRPHRMKTPLSIKRGTVAAVFIDLQEEHRQDKR
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHH
YLVEGFAGILANVQRLQEAARANHVPLQHWAYIVDLDKQDRPFHPLGADGKSAFSDKSDP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LTEICHEVAPARDEALLVKAEASAFRSGPAADQLKAAGIEWLVVAGVWTEACIDATVKDA
HHHHHHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHH
VARGFRVLLVKDACGSGSAAMHQTGILNLANRLYGGAVTDTDGACRLLAGETVTAWQVEG
HHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEECC
SVPLRFTFDNAAALYAEL
CCEEEEEECCCEEHEECC
>Mature Secondary Structure
MDDEGGEAPANHHRLRRCAHRNDADRKPRPHRMKTPLSIKRGTVAAVFIDLQEEHRQDKR
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHH
YLVEGFAGILANVQRLQEAARANHVPLQHWAYIVDLDKQDRPFHPLGADGKSAFSDKSDP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LTEICHEVAPARDEALLVKAEASAFRSGPAADQLKAAGIEWLVVAGVWTEACIDATVKDA
HHHHHHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHH
VARGFRVLLVKDACGSGSAAMHQTGILNLANRLYGGAVTDTDGACRLLAGETVTAWQVEG
HHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEECC
SVPLRFTFDNAAALYAEL
CCEEEEEECCCEEHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA