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The map label for this gene is bkdA1 [H]

Identifier: 13473769

GI number: 13473769

Start: 3557779

End: 3559011

Strand: Reverse

Name: bkdA1 [H]

Synonym: mll4473

Alternate gene names: 13473769

Gene position: 3559011-3557779 (Counterclockwise)

Preceding gene: 13473771

Following gene: 13473768

Centisome position: 50.58

GC content: 65.37

Gene sequence:

>1233_bases
ATGGCCGATGCTGGCATGTTGCGTTTCCATGTTCCGGAACCGGAAGTGCGGCCGGGCGGCACGCCTGATTTTTCCAACGT
GACCATCGCCGCGGCCGGTTCGGTGCCGCGCCCCGACATCGATGTCGATCCGCGCACCATCCGCGACATGGCCTTTTCCA
TCATCCGCGTGCTGAACCGCAATGGCGAAGCCGTCGGCCCGTGGGCCGGGCTGCTCTCCAATGAGGAGCTGCTCGAAGGC
CTGCGCCATATGATGACGCTCAGGACCTTCGACGCGCGCATGCAGATGGCGCAGCGCCAGGGCAAGACCTCCTTCTACAT
GCAGCATCTCGGCGAAGAGGCGGTGAGCTGCGCCTTCCGCAAGGCACTCGAGCCGGGCGACATGAATTTCCCGACCTATC
GGCAGGCCGGCCTGCTCATCGCCGACGGCTATCCGATGGTCACGATGATGAACCAGATCTATTCCAACGAGGCCGACCCG
CTGAAGGGCCGGCAGCTGCCGATCATGTATTCGTCGAAGGAGCACGGCTTCTTCTCGATCTCCGGCAATCTGGCGACGCA
GTACATCCAGGCGGTCGGCTGGGCGATGGCCTCGGCGATCTCGAACGATTCGAAGATCGCGGCGGCCTGGATCGGCGACG
GCTCGACGGCGGAATCCGATTTTCACTCGGCGCTGGTGTTCGCTTCCACCTACAAGGCGCCGGTCGTGCTCAATGTCGTC
AACAACCAGTGGGCGATCTCGACCTTTCAAGGCATAGCGCGCGGCGGCTCCGGCACCTTCGCGGCGCGCGGTCTCGGCTT
CGGCATCCCGTCGCTGCGTGTCGACGGCAATGACTATCTCGCCGTGCATGCGGTGGCCAAATGGGCGGCCGAGCGGGCGC
GGAAGAATCTCGGGCCCACGCTGGTCGAATACGTCACCTACCGCGCCGGCGCCCATTCCAGTTCGGACGATCCTTCGGCC
TACCGACCGAAGACCGAATCCGACGCCTGGCCACTCGGCGATCCGGTCGTGCGGCTGAAGAACCATCTGATCGGGCTCGG
GGTCTGGTCGGACGAACGCCACGCGCAGGCCGAGGCCGAAATCCTCGACACGGTGATCGCGGCGCAGAAGGAGGCGGAAA
GCCACGGCACGCTGCATGCCGGCGGCAAGCCGTCGACCCGCGACATGTTCGAGGGCCTCTATGCCGAGATGCCGCCGCAT
CTCAGGCGCCAGCGCCAGCAGGCGGGAGTCTGA

Upstream 100 bases:

>100_bases
GGATCGGCCGGCGCGGCCGGTCCGATGACCTTCGGCGAATTCGCACTGGACGCGCGGATGTGCAAATATCGGCGCCAGGC
AACATTGGAGGACGAAGGTC

Downstream 100 bases:

>100_bases
GCCATGCCGAGACGGACCATGATCGAGGCCATTCGTGACGCCATGGATGTGTCGATGGGACGTGACGACAAGGTCGTCGT
GTTCGGCGAGGATGTCGGCT

Product: 2-oxoisovalerate dehydrogenase (alpha subunit)

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MADAGMLRFHVPEPEVRPGGTPDFSNVTIAAAGSVPRPDIDVDPRTIRDMAFSIIRVLNRNGEAVGPWAGLLSNEELLEG
LRHMMTLRTFDARMQMAQRQGKTSFYMQHLGEEAVSCAFRKALEPGDMNFPTYRQAGLLIADGYPMVTMMNQIYSNEADP
LKGRQLPIMYSSKEHGFFSISGNLATQYIQAVGWAMASAISNDSKIAAAWIGDGSTAESDFHSALVFASTYKAPVVLNVV
NNQWAISTFQGIARGGSGTFAARGLGFGIPSLRVDGNDYLAVHAVAKWAAERARKNLGPTLVEYVTYRAGAHSSSDDPSA
YRPKTESDAWPLGDPVVRLKNHLIGLGVWSDERHAQAEAEILDTVIAAQKEAESHGTLHAGGKPSTRDMFEGLYAEMPPH
LRRQRQQAGV

Sequences:

>Translated_410_residues
MADAGMLRFHVPEPEVRPGGTPDFSNVTIAAAGSVPRPDIDVDPRTIRDMAFSIIRVLNRNGEAVGPWAGLLSNEELLEG
LRHMMTLRTFDARMQMAQRQGKTSFYMQHLGEEAVSCAFRKALEPGDMNFPTYRQAGLLIADGYPMVTMMNQIYSNEADP
LKGRQLPIMYSSKEHGFFSISGNLATQYIQAVGWAMASAISNDSKIAAAWIGDGSTAESDFHSALVFASTYKAPVVLNVV
NNQWAISTFQGIARGGSGTFAARGLGFGIPSLRVDGNDYLAVHAVAKWAAERARKNLGPTLVEYVTYRAGAHSSSDDPSA
YRPKTESDAWPLGDPVVRLKNHLIGLGVWSDERHAQAEAEILDTVIAAQKEAESHGTLHAGGKPSTRDMFEGLYAEMPPH
LRRQRQQAGV
>Mature_409_residues
ADAGMLRFHVPEPEVRPGGTPDFSNVTIAAAGSVPRPDIDVDPRTIRDMAFSIIRVLNRNGEAVGPWAGLLSNEELLEGL
RHMMTLRTFDARMQMAQRQGKTSFYMQHLGEEAVSCAFRKALEPGDMNFPTYRQAGLLIADGYPMVTMMNQIYSNEADPL
KGRQLPIMYSSKEHGFFSISGNLATQYIQAVGWAMASAISNDSKIAAAWIGDGSTAESDFHSALVFASTYKAPVVLNVVN
NQWAISTFQGIARGGSGTFAARGLGFGIPSLRVDGNDYLAVHAVAKWAAERARKNLGPTLVEYVTYRAGAHSSSDDPSAY
RPKTESDAWPLGDPVVRLKNHLIGLGVWSDERHAQAEAEILDTVIAAQKEAESHGTLHAGGKPSTRDMFEGLYAEMPPHL
RRQRQQAGV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=404, Percent_Identity=36.6336633663366, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI258645172, Length=404, Percent_Identity=36.3861386138614, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4885543, Length=297, Percent_Identity=26.2626262626263, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4505685, Length=377, Percent_Identity=24.6684350132626, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291084742, Length=355, Percent_Identity=25.0704225352113, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291084744, Length=384, Percent_Identity=24.21875, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI291084757, Length=378, Percent_Identity=23.5449735449735, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI86563355, Length=360, Percent_Identity=38.8888888888889, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI86563357, Length=360, Percent_Identity=38.8888888888889, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17536047, Length=309, Percent_Identity=25.2427184466019, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI32564172, Length=309, Percent_Identity=25.2427184466019, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321026, Length=286, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355903, Length=362, Percent_Identity=37.8453038674033, Blast_Score=218, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24639748, Length=285, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639744, Length=309, Percent_Identity=23.6245954692557, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571106, Length=309, Percent_Identity=23.6245954692557, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639740, Length=309, Percent_Identity=23.6245954692557, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639746, Length=297, Percent_Identity=23.9057239057239, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 44664; Mature: 44533

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAGMLRFHVPEPEVRPGGTPDFSNVTIAAAGSVPRPDIDVDPRTIRDMAFSIIRVLNR
CCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NGEAVGPWAGLLSNEELLEGLRHMMTLRTFDARMQMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALEPGDMNFPTYRQAGLLIADGYPMVTMMNQIYSNEADPLKGRQLPIMYSSKEHGFFSI
HHCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEE
SGNLATQYIQAVGWAMASAISNDSKIAAAWIGDGSTAESDFHSALVFASTYKAPVVLNVV
ECHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHEEECCCCCEEEEEE
NNQWAISTFQGIARGGSGTFAARGLGFGIPSLRVDGNDYLAVHAVAKWAAERARKNLGPT
CCCEEHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHCCCHH
LVEYVTYRAGAHSSSDDPSAYRPKTESDAWPLGDPVVRLKNHLIGLGVWSDERHAQAEAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCHHHHHHHH
ILDTVIAAQKEAESHGTLHAGGKPSTRDMFEGLYAEMPPHLRRQRQQAGV
HHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADAGMLRFHVPEPEVRPGGTPDFSNVTIAAAGSVPRPDIDVDPRTIRDMAFSIIRVLNR
CCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NGEAVGPWAGLLSNEELLEGLRHMMTLRTFDARMQMAQRQGKTSFYMQHLGEEAVSCAFR
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALEPGDMNFPTYRQAGLLIADGYPMVTMMNQIYSNEADPLKGRQLPIMYSSKEHGFFSI
HHCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEE
SGNLATQYIQAVGWAMASAISNDSKIAAAWIGDGSTAESDFHSALVFASTYKAPVVLNVV
ECHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHEEECCCCCEEEEEE
NNQWAISTFQGIARGGSGTFAARGLGFGIPSLRVDGNDYLAVHAVAKWAAERARKNLGPT
CCCEEHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHCCCHH
LVEYVTYRAGAHSSSDDPSAYRPKTESDAWPLGDPVVRLKNHLIGLGVWSDERHAQAEAE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCHHHHHHHH
ILDTVIAAQKEAESHGTLHAGGKPSTRDMFEGLYAEMPPHLRRQRQQAGV
HHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]