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The map label for this gene is alkJ [H]

Identifier: 13472341

GI number: 13472341

Start: 2105559

End: 2107175

Strand: Direct

Name: alkJ [H]

Synonym: mlr2604

Alternate gene names: 13472341

Gene position: 2105559-2107175 (Clockwise)

Preceding gene: 13472337

Following gene: 13472342

Centisome position: 29.93

GC content: 65.06

Gene sequence:

>1617_bases
ATGCAGATCTATGACTTCATCATCGTCGGCTCCGGCTCGGCCGGCTCGGTGCTCGCCGAGAGACTGTCGGCCTCGGGCCG
CTTCTCGGTGCTGGTGCTGGAGGCCGGCGGCACGGACCGGCGCTTCTACGTGCAGATGCCGCTCGGCTATGGCAAGACCT
TCTTCGACCCGGCGGTCAACTGGAACTACAAGACGGAAGCCGATCCCGGTCTCGGCGGCAATGTCGACCACTGGCCGCGC
GGCAAGCTGCTCGGCGGCTCGAGTTCGATCAACGCCATGGTCTGGATTCGGGGTGCGCGCGAGGATTTCGACGACTGGCG
CGCCGCCGGCAATCCCGGCTGGAGCTATGACGAGCTGTTGCCGATCTTCAAGGCGCTCGAGGACAATGAGGCCGGCGCCG
ACAGATGGCGCGGCACCGGTGGTCCCCTGCACATCAGCGATACGGCCAACGCCGTCCACCCGCTGACAAAGCGTTATCTC
GCCGCCGGCCAGCAGGCCGGCCTGCCGCTCAACCCCGATTTCAACGGCGCTGCCCAGGAAGGCGTCGGCACCTACCAGAT
CTCGACCAAGAACGGCCGCCGCATGTCCGCCGCGCGCGCCTTCCTGCGCCCGGCGATGAAGCGCGGCAATGTCCGCGTCG
AGACCAACGCGCTGGCCAGCCGCATCCTGTTCGAAGGCAAACGCGCGGTCGGCATCGAATATCTGCAGAACGGTCAGACG
AAGACGGCGCGGGCCGGCCGCGAGGTGATCCTCTCCGCCGGCTCGATCAACTCGCCGCAGCTGCTGCAATTGTCGGGCGT
CGGCCCCTCGGCGCTTCTGAAAGGGCTGGGCATCGCTGTCGTCCACGCCAACGAGAATGTCGGTGCGCATCTGCAGGACC
ATGTCGGCATCAACTACACCTTCAAGGGCAAGGTGCCGACGCTCAACCAGATCCTGCGTCCCTGGTGGGGCAAGCTGCTG
GTCGGCATGCAGTACATACTGACGCGCTCGGGACCGCTGTCGCTGTCGATGAACCATGGCGGCGGTTTCTTCCGCACCGA
CCCGGCGTTCTCGCGCCCCAACATGCAGCTCTATTTCCAGGCCTTCTCGACGGTCATCCCGAAGAGCGGCGAGCGGCCGA
TCCTGACGCCGGACCCATGGCCGGGCTTCTCCATCGGCCTGTCCAATTGCCGCCCGTCGAGCCGGGGCGAGATCATGATC
CGCTCCAGCAATCCGCTGGACTATCCGAAGATCACCGCCAATGCCTATTCTACCAATGCCGATGTCGAGGAGATGCTGGC
GGCGGTGAAGTTCGTGCGCAAGATCGCCTCGATGCCAGCGTTGGCGGAAATCATCCAGGAGGAAGTCCTGCCCGGCCCGT
CGATCCAGTCGGACGCCGACCTGATCACCGATTTCAGGAAACGCTCCGGCACTGTCTATCACCCGGTCTCGACCTGCCGC
ATGGGGCCCGATCCCACGCGCGCCGCCGTCGATCCGCGTCTGAAGGTGCATGGGCTTGAAGGCTTGCGCGTCATCGACGC
CTCGATCTTTCCCGACAACATCACCGGCAACACCAACGCCGCCTCGGTCATGACCGGGTGGAAGGGTGCCGAACTGGTTC
TGGAGGACCAAAAATGA

Upstream 100 bases:

>100_bases
CCAGTCGCTTTTTTCACAACGCATGCCGGAAGGCCCGGCGACGCGGCGAACCCGTCGCGTCTAGGCTGCGGCCAGTTTTC
CGGAGCCGCCCGTTTCAATC

Downstream 100 bases:

>100_bases
AGATCACCGACGTCAAGACCTGGGTTGTCGGCAACCCGCCGCCCGGCATCGGCGGCAAGTATTTCATCTTCGTCAAACTG
ACCACCGACGGCGGCGTGGT

Product: alcohol dehydrogenase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MQIYDFIIVGSGSAGSVLAERLSASGRFSVLVLEAGGTDRRFYVQMPLGYGKTFFDPAVNWNYKTEADPGLGGNVDHWPR
GKLLGGSSSINAMVWIRGAREDFDDWRAAGNPGWSYDELLPIFKALEDNEAGADRWRGTGGPLHISDTANAVHPLTKRYL
AAGQQAGLPLNPDFNGAAQEGVGTYQISTKNGRRMSAARAFLRPAMKRGNVRVETNALASRILFEGKRAVGIEYLQNGQT
KTARAGREVILSAGSINSPQLLQLSGVGPSALLKGLGIAVVHANENVGAHLQDHVGINYTFKGKVPTLNQILRPWWGKLL
VGMQYILTRSGPLSLSMNHGGGFFRTDPAFSRPNMQLYFQAFSTVIPKSGERPILTPDPWPGFSIGLSNCRPSSRGEIMI
RSSNPLDYPKITANAYSTNADVEEMLAAVKFVRKIASMPALAEIIQEEVLPGPSIQSDADLITDFRKRSGTVYHPVSTCR
MGPDPTRAAVDPRLKVHGLEGLRVIDASIFPDNITGNTNAASVMTGWKGAELVLEDQK

Sequences:

>Translated_538_residues
MQIYDFIIVGSGSAGSVLAERLSASGRFSVLVLEAGGTDRRFYVQMPLGYGKTFFDPAVNWNYKTEADPGLGGNVDHWPR
GKLLGGSSSINAMVWIRGAREDFDDWRAAGNPGWSYDELLPIFKALEDNEAGADRWRGTGGPLHISDTANAVHPLTKRYL
AAGQQAGLPLNPDFNGAAQEGVGTYQISTKNGRRMSAARAFLRPAMKRGNVRVETNALASRILFEGKRAVGIEYLQNGQT
KTARAGREVILSAGSINSPQLLQLSGVGPSALLKGLGIAVVHANENVGAHLQDHVGINYTFKGKVPTLNQILRPWWGKLL
VGMQYILTRSGPLSLSMNHGGGFFRTDPAFSRPNMQLYFQAFSTVIPKSGERPILTPDPWPGFSIGLSNCRPSSRGEIMI
RSSNPLDYPKITANAYSTNADVEEMLAAVKFVRKIASMPALAEIIQEEVLPGPSIQSDADLITDFRKRSGTVYHPVSTCR
MGPDPTRAAVDPRLKVHGLEGLRVIDASIFPDNITGNTNAASVMTGWKGAELVLEDQK
>Mature_538_residues
MQIYDFIIVGSGSAGSVLAERLSASGRFSVLVLEAGGTDRRFYVQMPLGYGKTFFDPAVNWNYKTEADPGLGGNVDHWPR
GKLLGGSSSINAMVWIRGAREDFDDWRAAGNPGWSYDELLPIFKALEDNEAGADRWRGTGGPLHISDTANAVHPLTKRYL
AAGQQAGLPLNPDFNGAAQEGVGTYQISTKNGRRMSAARAFLRPAMKRGNVRVETNALASRILFEGKRAVGIEYLQNGQT
KTARAGREVILSAGSINSPQLLQLSGVGPSALLKGLGIAVVHANENVGAHLQDHVGINYTFKGKVPTLNQILRPWWGKLL
VGMQYILTRSGPLSLSMNHGGGFFRTDPAFSRPNMQLYFQAFSTVIPKSGERPILTPDPWPGFSIGLSNCRPSSRGEIMI
RSSNPLDYPKITANAYSTNADVEEMLAAVKFVRKIASMPALAEIIQEEVLPGPSIQSDADLITDFRKRSGTVYHPVSTCR
MGPDPTRAAVDPRLKVHGLEGLRVIDASIFPDNITGNTNAASVMTGWKGAELVLEDQK

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=540, Percent_Identity=42.037037037037, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1786503, Length=538, Percent_Identity=39.0334572490706, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17532301, Length=553, Percent_Identity=33.4538878842676, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24642042, Length=580, Percent_Identity=37.0689655172414, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=35.8391608391608, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=35.8391608391608, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24642055, Length=577, Percent_Identity=37.6083188908146, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI17137792, Length=555, Percent_Identity=36.7567567567568, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=36.0281195079086, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24642037, Length=571, Percent_Identity=37.1278458844133, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24642048, Length=568, Percent_Identity=35.5633802816901, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24642039, Length=575, Percent_Identity=34.9565217391304, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24650267, Length=569, Percent_Identity=34.9736379613357, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI18859995, Length=571, Percent_Identity=35.0262697022767, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI18859993, Length=576, Percent_Identity=33.6805555555556, Blast_Score=268, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642051, Length=584, Percent_Identity=32.8767123287671, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24642035, Length=303, Percent_Identity=41.2541254125413, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24642057, Length=582, Percent_Identity=29.553264604811, Blast_Score=165, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24645930, Length=585, Percent_Identity=26.8376068376068, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58277; Mature: 58277

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYDFIIVGSGSAGSVLAERLSASGRFSVLVLEAGGTDRRFYVQMPLGYGKTFFDPAVN
CEEEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHCCCCCC
WNYKTEADPGLGGNVDHWPRGKLLGGSSSINAMVWIRGAREDFDDWRAAGNPGWSYDELL
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHH
PIFKALEDNEAGADRWRGTGGPLHISDTANAVHPLTKRYLAAGQQAGLPLNPDFNGAAQE
HHHHHHCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
GVGTYQISTKNGRRMSAARAFLRPAMKRGNVRVETNALASRILFEGKRAVGIEYLQNGQT
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHCCHHHHHCCCC
KTARAGREVILSAGSINSPQLLQLSGVGPSALLKGLGIAVVHANENVGAHLQDHVGINYT
HHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHCCCCEEEEECCCCCCCCCHHCCCEEEE
FKGKVPTLNQILRPWWGKLLVGMQYILTRSGPLSLSMNHGGGFFRTDPAFSRPNMQLYFQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCEEHHH
AFSTVIPKSGERPILTPDPWPGFSIGLSNCRPSSRGEIMIRSSNPLDYPKITANAYSTNA
HHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCC
DVEEMLAAVKFVRKIASMPALAEIIQEEVLPGPSIQSDADLITDFRKRSGTVYHPVSTCR
CHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECHHHCC
MGPDPTRAAVDPRLKVHGLEGLRVIDASIFPDNITGNTNAASVMTGWKGAELVLEDQK
CCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCHHEEECCCCCEEEEECCC
>Mature Secondary Structure
MQIYDFIIVGSGSAGSVLAERLSASGRFSVLVLEAGGTDRRFYVQMPLGYGKTFFDPAVN
CEEEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHCCCCCC
WNYKTEADPGLGGNVDHWPRGKLLGGSSSINAMVWIRGAREDFDDWRAAGNPGWSYDELL
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCCHHHHH
PIFKALEDNEAGADRWRGTGGPLHISDTANAVHPLTKRYLAAGQQAGLPLNPDFNGAAQE
HHHHHHCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHC
GVGTYQISTKNGRRMSAARAFLRPAMKRGNVRVETNALASRILFEGKRAVGIEYLQNGQT
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHCCHHHHHCCCC
KTARAGREVILSAGSINSPQLLQLSGVGPSALLKGLGIAVVHANENVGAHLQDHVGINYT
HHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHCCCCEEEEECCCCCCCCCHHCCCEEEE
FKGKVPTLNQILRPWWGKLLVGMQYILTRSGPLSLSMNHGGGFFRTDPAFSRPNMQLYFQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCEEHHH
AFSTVIPKSGERPILTPDPWPGFSIGLSNCRPSSRGEIMIRSSNPLDYPKITANAYSTNA
HHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCC
DVEEMLAAVKFVRKIASMPALAEIIQEEVLPGPSIQSDADLITDFRKRSGTVYHPVSTCR
CHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEECHHHCC
MGPDPTRAAVDPRLKVHGLEGLRVIDASIFPDNITGNTNAASVMTGWKGAELVLEDQK
CCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCHHEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]