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The map label for this gene is pcm [H]

Identifier: 13472265

GI number: 13472265

Start: 2031476

End: 2032318

Strand: Direct

Name: pcm [H]

Synonym: mlr2502

Alternate gene names: 13472265

Gene position: 2031476-2032318 (Clockwise)

Preceding gene: 13472264

Following gene: 13472266

Centisome position: 28.87

GC content: 65.12

Gene sequence:

>843_bases
ATGAGCAAAATTGAAGACATCAGGAAATTCTACGCCCGGCTGATGGCGGCCAATGCCGCCTCATCGGATCCACGCCTGGA
GCAGGTCTTCGCCACTGTTCCGCGCGAAGCCTTTCTCGGCCCGGGGCCGTGGACGATCGTCGCCGGCAGCGGCAGGGTTA
CCACGCCGAGCGCCGACCCGGCCCATATCTACCAGAACGTGCTGGTGGCGCTCGATGCCGACAAGGGCATCAACAATGGC
GAGCCGTTCCTGCACGCCATGTGGATCGGAAAATTGGCGCCGAAACCCGGCGAGGCCGTCACGCATGTCGGCGCCGGCAC
CGGCTACTACTCCGCCGTGCTGGCCAGGCTGGTGTCACCTGGCGGCACCGTCACCGCCTTCGAGCTCGAAGGCAGGCTGG
CCGATCTGGCGCGCAAAAATCTCGAAATCTACGGCAATGCGACGGTCATCCATGGCGACGCCGTGACCAGGCCCTTGCCG
CCATCCGACATCATCTATGTCAACGCCGGCGTCGTCGCCCCGCCGGTCGGATGGCTTAAGGCGTTGCGCCCCGGCGGCCG
CATGATCTTCCCCTGGCGCCCATCCGAACGCGTCCCGCTGGCGGTGATGGTGACCCGCACCGAAAAGGGCTTTGCCTGCG
ATCCCTTCATGCGCTCCTGGTTCATCCCCTGCATCGGTGCTTCGGTTGCGGATCTTGCGGCGAAGATCCCGACACCCGAG
CAAGCGACGCGCAGCCGGTCGATATGGCTGACGCAGCACAAGGCGCCGGATCGCACCGCTACGGCGATCTTCGGCGACGT
CTGGTTCTCGACGAAAGACGTCCGTGCCAAACCCGGCAGCTGA

Upstream 100 bases:

>100_bases
GCTCAATTTCAAGCTGCTCTACGACACCCTGTTCGGGGTTGGCTGAGTCTGGCGCGAATCCGGAGACAAACCGCAAGATC
GCGCTATCTTTCCCGGTGCC

Downstream 100 bases:

>100_bases
GGCATCGATGCGCGGATTTGGCCAAAATTTCTCAGGCCCTGTCGGGGCGGGTGCGGGCCAGCCGTCCTTGGGCCACAATC
CGCCCGCGACGGCGTGGATG

Product: O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSKIEDIRKFYARLMAANAASSDPRLEQVFATVPREAFLGPGPWTIVAGSGRVTTPSADPAHIYQNVLVALDADKGINNG
EPFLHAMWIGKLAPKPGEAVTHVGAGTGYYSAVLARLVSPGGTVTAFELEGRLADLARKNLEIYGNATVIHGDAVTRPLP
PSDIIYVNAGVVAPPVGWLKALRPGGRMIFPWRPSERVPLAVMVTRTEKGFACDPFMRSWFIPCIGASVADLAAKIPTPE
QATRSRSIWLTQHKAPDRTATAIFGDVWFSTKDVRAKPGS

Sequences:

>Translated_280_residues
MSKIEDIRKFYARLMAANAASSDPRLEQVFATVPREAFLGPGPWTIVAGSGRVTTPSADPAHIYQNVLVALDADKGINNG
EPFLHAMWIGKLAPKPGEAVTHVGAGTGYYSAVLARLVSPGGTVTAFELEGRLADLARKNLEIYGNATVIHGDAVTRPLP
PSDIIYVNAGVVAPPVGWLKALRPGGRMIFPWRPSERVPLAVMVTRTEKGFACDPFMRSWFIPCIGASVADLAAKIPTPE
QATRSRSIWLTQHKAPDRTATAIFGDVWFSTKDVRAKPGS
>Mature_279_residues
SKIEDIRKFYARLMAANAASSDPRLEQVFATVPREAFLGPGPWTIVAGSGRVTTPSADPAHIYQNVLVALDADKGINNGE
PFLHAMWIGKLAPKPGEAVTHVGAGTGYYSAVLARLVSPGGTVTAFELEGRLADLARKNLEIYGNATVIHGDAVTRPLPP
SDIIYVNAGVVAPPVGWLKALRPGGRMIFPWRPSERVPLAVMVTRTEKGFACDPFMRSWFIPCIGASVADLAAKIPTPEQ
ATRSRSIWLTQHKAPDRTATAIFGDVWFSTKDVRAKPGS

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682
- InterPro:   IPR009107 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 30126; Mature: 29995

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIEDIRKFYARLMAANAASSDPRLEQVFATVPREAFLGPGPWTIVAGSGRVTTPSADP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCEEEEECCCEEECCCCCH
AHIYQNVLVALDADKGINNGEPFLHAMWIGKLAPKPGEAVTHVGAGTGYYSAVLARLVSP
HHHHHEEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCC
GGTVTAFELEGRLADLARKNLEIYGNATVIHGDAVTRPLPPSDIIYVNAGVVAPPVGWLK
CCCEEEEEECCHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHH
ALRPGGRMIFPWRPSERVPLAVMVTRTEKGFACDPFMRSWFIPCIGASVADLAAKIPTPE
HHCCCCCEEECCCCCCCCCEEEEEEECCCCCEECHHHHHHHHHHCCCHHHHHHHCCCCCH
QATRSRSIWLTQHKAPDRTATAIFGDVWFSTKDVRAKPGS
HHHCCCEEEEEECCCCCCHHHEEEEHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKIEDIRKFYARLMAANAASSDPRLEQVFATVPREAFLGPGPWTIVAGSGRVTTPSADP
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCEEEEECCCEEECCCCCH
AHIYQNVLVALDADKGINNGEPFLHAMWIGKLAPKPGEAVTHVGAGTGYYSAVLARLVSP
HHHHHEEEEEEECCCCCCCCCCEEEEEEHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCC
GGTVTAFELEGRLADLARKNLEIYGNATVIHGDAVTRPLPPSDIIYVNAGVVAPPVGWLK
CCCEEEEEECCHHHHHHHCCEEEEECEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHH
ALRPGGRMIFPWRPSERVPLAVMVTRTEKGFACDPFMRSWFIPCIGASVADLAAKIPTPE
HHCCCCCEEECCCCCCCCCEEEEEEECCCCCEECHHHHHHHHHHCCCHHHHHHHCCCCCH
QATRSRSIWLTQHKAPDRTATAIFGDVWFSTKDVRAKPGS
HHHCCCEEEEEECCCCCCHHHEEEEHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550470; 10360571; 9784234 [H]