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The map label for this gene is est [H]

Identifier: 13471782

GI number: 13471782

Start: 1539367

End: 1540317

Strand: Reverse

Name: est [H]

Synonym: mll1869

Alternate gene names: 13471782

Gene position: 1540317-1539367 (Counterclockwise)

Preceding gene: 13471783

Following gene: 13471781

Centisome position: 21.89

GC content: 67.09

Gene sequence:

>951_bases
ATGGCGAGCATCGAGAGCGAGGCAAACAGGAACCACTACGCGGCGATCGGCGCCAATGCCGGCAAACTGAGCCCGCAGGC
GTTCGTTGAGTTCAACGATAGCAGTTGGACGGCGCTGACCGGCGAACCCGGCGGCGTCGACTATATCGAGGTGGATGCCG
GCGGCGTGCCTGGGCTATGGGTCGTGCCGAAGGGCGCCGACGAGCGGCGCGTGCTGTTTTATGCGCATGGCGGCGGCTTC
CTCGGCGGCTCGATCTACACCCACCGCAAGCTGGTCGGTCATCTGGCCAAGGCCGTCGGCTGCCGGGCCTTGCTGTATGG
CTATCCGCTGGCCAGCCAGGCCAAGTATCCGGCCCAGCTCGAGGCGGCGATGGCAGCCTGGGACTGGCTCATCGACCAGG
ACTTCGACACCAGGCGGATCGCGCTTGCCGCAGATTCCTGCGGCGCGGTGCTGACCTATGGCGTGCTGCAGCGGCTGCGG
GCGCAGGAGCGTCCGCTGCCCGCCGCCACGCTGATCATCTCCGGCTGGTTCGACATGGCGCTGACCGCTGCCAGCTATGA
AACCAACCGCGAAAAGGATCCTTTCTTCGCCAAGGGCGGCGTCGACTGGCTGGTGACCAGCTTCATCGGCGACTACGATC
GGCTGGATCCGGAGGTCAGCCCGCTCTATGCCGATTTGAGTGGCTTTCCGCCAGTCTTCCTGCAGGCCGGCGCCGATGAG
ACGCTGGTCGACGAAAGCCGCATGTTCGCCGAGCGCGCGCGGCAGGCTGGCGTCGAGACGCGCCTCGACATCTTCGACGA
CATGCTGCACTCCTTCCAGATGATGGCCGGCCGGGCGCCGGAAGCCGATGACGCGATCGGCCGCTTGGCCGCCTGGGTGC
GGCCGAGGCTTGGGCTGCCGGATGCCGGTGATAACGCCGTCAGCGACAAGGTTGCCGGCAGGGCGGCGTAG

Upstream 100 bases:

>100_bases
GCGGAAAAATCGTCCGCGGCGACGTATCCTTTTTGTCAGCCCGTCCGTCTTTGCTTCTCGAAAGGTCGCGCGGTCCGCGA
TCCATAACCAGGAGAGCATC

Downstream 100 bases:

>100_bases
GCATGGGCTCGGGTCGCACTTGGTGCTTGTGGATGCGCGGCGCTTGCGCGATGCTGCGGCAAGGCTGAGCGACGCGCCGC
GATGAACCAGCAGATCCGCA

Product: esterase/deacetylase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MASIESEANRNHYAAIGANAGKLSPQAFVEFNDSSWTALTGEPGGVDYIEVDAGGVPGLWVVPKGADERRVLFYAHGGGF
LGGSIYTHRKLVGHLAKAVGCRALLYGYPLASQAKYPAQLEAAMAAWDWLIDQDFDTRRIALAADSCGAVLTYGVLQRLR
AQERPLPAATLIISGWFDMALTAASYETNREKDPFFAKGGVDWLVTSFIGDYDRLDPEVSPLYADLSGFPPVFLQAGADE
TLVDESRMFAERARQAGVETRLDIFDDMLHSFQMMAGRAPEADDAIGRLAAWVRPRLGLPDAGDNAVSDKVAGRAA

Sequences:

>Translated_316_residues
MASIESEANRNHYAAIGANAGKLSPQAFVEFNDSSWTALTGEPGGVDYIEVDAGGVPGLWVVPKGADERRVLFYAHGGGF
LGGSIYTHRKLVGHLAKAVGCRALLYGYPLASQAKYPAQLEAAMAAWDWLIDQDFDTRRIALAADSCGAVLTYGVLQRLR
AQERPLPAATLIISGWFDMALTAASYETNREKDPFFAKGGVDWLVTSFIGDYDRLDPEVSPLYADLSGFPPVFLQAGADE
TLVDESRMFAERARQAGVETRLDIFDDMLHSFQMMAGRAPEADDAIGRLAAWVRPRLGLPDAGDNAVSDKVAGRAA
>Mature_315_residues
ASIESEANRNHYAAIGANAGKLSPQAFVEFNDSSWTALTGEPGGVDYIEVDAGGVPGLWVVPKGADERRVLFYAHGGGFL
GGSIYTHRKLVGHLAKAVGCRALLYGYPLASQAKYPAQLEAAMAAWDWLIDQDFDTRRIALAADSCGAVLTYGVLQRLRA
QERPLPAATLIISGWFDMALTAASYETNREKDPFFAKGGVDWLVTSFIGDYDRLDPEVSPLYADLSGFPPVFLQAGADET
LVDESRMFAERARQAGVETRLDIFDDMLHSFQMMAGRAPEADDAIGRLAAWVRPRLGLPDAGDNAVSDKVAGRAA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34122; Mature: 33990

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIESEANRNHYAAIGANAGKLSPQAFVEFNDSSWTALTGEPGGVDYIEVDAGGVPGLW
CCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VVPKGADERRVLFYAHGGGFLGGSIYTHRKLVGHLAKAVGCRALLYGYPLASQAKYPAQL
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
EAAMAAWDWLIDQDFDTRRIALAADSCGAVLTYGVLQRLRAQERPLPAATLIISGWFDMA
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHEEECHHHHH
LTAASYETNREKDPFFAKGGVDWLVTSFIGDYDRLDPEVSPLYADLSGFPPVFLQAGADE
HHHHHCCCCCCCCCCEECCCHHHHHHHHHCCHHHCCCCCCHHHHCCCCCCCEEEECCCCC
TLVDESRMFAERARQAGVETRLDIFDDMLHSFQMMAGRAPEADDAIGRLAAWVRPRLGLP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
DAGDNAVSDKVAGRAA
CCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ASIESEANRNHYAAIGANAGKLSPQAFVEFNDSSWTALTGEPGGVDYIEVDAGGVPGLW
CCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECCCCCCCCEEEECCCCCCCEE
VVPKGADERRVLFYAHGGGFLGGSIYTHRKLVGHLAKAVGCRALLYGYPLASQAKYPAQL
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
EAAMAAWDWLIDQDFDTRRIALAADSCGAVLTYGVLQRLRAQERPLPAATLIISGWFDMA
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHEEECHHHHH
LTAASYETNREKDPFFAKGGVDWLVTSFIGDYDRLDPEVSPLYADLSGFPPVFLQAGADE
HHHHHCCCCCCCCCCEECCCHHHHHHHHHCCHHHCCCCCCHHHHCCCCCCCEEEECCCCC
TLVDESRMFAERARQAGVETRLDIFDDMLHSFQMMAGRAPEADDAIGRLAAWVRPRLGLP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
DAGDNAVSDKVAGRAA
CCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]