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The map label for this gene is azoB [H]

Identifier: 13471783

GI number: 13471783

Start: 1540423

End: 1541304

Strand: Reverse

Name: azoB [H]

Synonym: mll1871

Alternate gene names: 13471783

Gene position: 1541304-1540423 (Counterclockwise)

Preceding gene: 13471784

Following gene: 13471782

Centisome position: 21.91

GC content: 67.01

Gene sequence:

>882_bases
ATGATCCTGGTGACGGGCGCCACGGGGCTGAACGGCAAGGCGGTCATGCGCGAGTTCGCGCGGCACAAGCATGAGGTGAG
GGCGCTGGTGCGCGATCCCGACCGGGCGTCGGTGGCAGGGCTCGGCGGACTTGCCGGCGTCGAACTGGTCACGGGCGACA
TGCGCCAGGCGGACACGCTTGGCGCGGCACTCGACGGCATCGATCGCGTGCTGATGATTTCGACCGCCGCCGACGACATG
ACGGAAACGCAGTGCCGCTTCGTCGATGCCTGCAGGCAGGCCGGCGTCGCGCATGTGGTGAAGTTCTCCGGCGCCGAATC
CAACATCGGCTACGACGCGACGAAGTTCCGCTTCACCCGCATGCATGAGGAGGTCGAACGCTATCTGGAAGCGGCCGGCA
TGGCATGGACGCATCTGCGCCCCAGCCAGTTCATGCAGGTCTACCTGCGCGACGCGCCGACCATTGCCGCCGAGGGCGCC
TTCTATCTCGCGCTCGGCGAGACCGAACTGTCGCCGGTCGATGTCGAGGATATCGCCAAGGTGGCTTTCCGGCTGCTGCG
CGATGGCGGCCATGCAGGCGAGAGCCTCGATATGACCGGGCCGCAGGCGCTGACGATGAGCGATATCGCGGCGCTTATAT
CCAAGGCGATCGGCAAGCCGGTTCGCTATGTCGATGTCAGCCCGGCAGAGCGGCGGCGCAACCTGCTGGCCAGCGGCGTT
CCAGCCGGCTTCGCCGACGCGCTGGACGAACAACTGGCCGAGCGGTTGCGCCGGCCGAAATCGCGGGTGCATCTGGCGAC
GCACGACATGTTCGGCGTGCGGCCGACGCCATTCGTTGAATTCGCCCAACGCCACGCCGCGATGTTTCGGGGCGAGGGTT
AG

Upstream 100 bases:

>100_bases
GTCAAAAAACTGTTTGGGCAGAATCCTTTGCCCTCGTCTCTTCGTCTTCAAGGTGCGGTCGCCGGCGTGGCGGCCGCCTC
AATCCCAGACGGAGATGACG

Downstream 100 bases:

>100_bases
CCGGGGCGGAAAAATCGTCCGCGGCGACGTATCCTTTTTGTCAGCCCGTCCGTCTTTGCTTCTCGAAAGGTCGCGCGGTC
CGCGATCCATAACCAGGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MILVTGATGLNGKAVMREFARHKHEVRALVRDPDRASVAGLGGLAGVELVTGDMRQADTLGAALDGIDRVLMISTAADDM
TETQCRFVDACRQAGVAHVVKFSGAESNIGYDATKFRFTRMHEEVERYLEAAGMAWTHLRPSQFMQVYLRDAPTIAAEGA
FYLALGETELSPVDVEDIAKVAFRLLRDGGHAGESLDMTGPQALTMSDIAALISKAIGKPVRYVDVSPAERRRNLLASGV
PAGFADALDEQLAERLRRPKSRVHLATHDMFGVRPTPFVEFAQRHAAMFRGEG

Sequences:

>Translated_293_residues
MILVTGATGLNGKAVMREFARHKHEVRALVRDPDRASVAGLGGLAGVELVTGDMRQADTLGAALDGIDRVLMISTAADDM
TETQCRFVDACRQAGVAHVVKFSGAESNIGYDATKFRFTRMHEEVERYLEAAGMAWTHLRPSQFMQVYLRDAPTIAAEGA
FYLALGETELSPVDVEDIAKVAFRLLRDGGHAGESLDMTGPQALTMSDIAALISKAIGKPVRYVDVSPAERRRNLLASGV
PAGFADALDEQLAERLRRPKSRVHLATHDMFGVRPTPFVEFAQRHAAMFRGEG
>Mature_293_residues
MILVTGATGLNGKAVMREFARHKHEVRALVRDPDRASVAGLGGLAGVELVTGDMRQADTLGAALDGIDRVLMISTAADDM
TETQCRFVDACRQAGVAHVVKFSGAESNIGYDATKFRFTRMHEEVERYLEAAGMAWTHLRPSQFMQVYLRDAPTIAAEGA
FYLALGETELSPVDVEDIAKVAFRLLRDGGHAGESLDMTGPQALTMSDIAALISKAIGKPVRYVDVSPAERRRNLLASGV
PAGFADALDEQLAERLRRPKSRVHLATHDMFGVRPTPFVEFAQRHAAMFRGEG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=256, Percent_Identity=30.46875, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31853; Mature: 31853

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGATGLNGKAVMREFARHKHEVRALVRDPDRASVAGLGGLAGVELVTGDMRQADTL
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCHHHHHHH
GAALDGIDRVLMISTAADDMTETQCRFVDACRQAGVAHVVKFSGAESNIGYDATKFRFTR
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHH
MHEEVERYLEAAGMAWTHLRPSQFMQVYLRDAPTIAAEGAFYLALGETELSPVDVEDIAK
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCHHHHHH
VAFRLLRDGGHAGESLDMTGPQALTMSDIAALISKAIGKPVRYVDVSPAERRRNLLASGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCC
PAGFADALDEQLAERLRRPKSRVHLATHDMFGVRPTPFVEFAQRHAAMFRGEG
CCCHHHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILVTGATGLNGKAVMREFARHKHEVRALVRDPDRASVAGLGGLAGVELVTGDMRQADTL
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCHHHHHHH
GAALDGIDRVLMISTAADDMTETQCRFVDACRQAGVAHVVKFSGAESNIGYDATKFRFTR
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHH
MHEEVERYLEAAGMAWTHLRPSQFMQVYLRDAPTIAAEGAFYLALGETELSPVDVEDIAK
HHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCHHHHHH
VAFRLLRDGGHAGESLDMTGPQALTMSDIAALISKAIGKPVRYVDVSPAERRRNLLASGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCC
PAGFADALDEQLAERLRRPKSRVHLATHDMFGVRPTPFVEFAQRHAAMFRGEG
CCCHHHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]