Click here to switch to the map view.

The map label for this gene is ntrB [H]

Identifier: 13470631

GI number: 13470631

Start: 282038

End: 283180

Strand: Direct

Name: ntrB [H]

Synonym: mlr0397

Alternate gene names: 13470631

Gene position: 282038-283180 (Clockwise)

Preceding gene: 13470630

Following gene: 13470632

Centisome position: 4.01

GC content: 62.9

Gene sequence:

>1143_bases
ATGAACGCCATCGCTGGCCAGGGAGCTGAAATGGTGGACGCCGCCCAGATCGTCCTCAACACCATCCGCCGCCCGGTGAT
CATGATCAGCGAGGAAGGCTTCATCACCTTCGCCAATGCCGATGCCGAGGACTTTTTTCGCTCCAGCGCGACCATGCTGG
CGCGCAACACCCTGTCCAAGCTCGTCCCCTTCGGCAGTCCGCTGCTGACCTTGGTCGATCAGGTGCGCGAGCGCCGGGCT
CCGGTCAACGAGTATCGGGTCGATGTGTCGTCGCCACGCCTCGGCATCGAGAAGGTCGTCGATCTCTATGTCGCGCCGGT
GCCGGAATTCCCGGGCTCCGTCGTGGTCATGTTCCAGGAACGGTCCATGGCCGACAAGATCGACCGGCAGATGACGCATC
GCGGTGCCGCGCGCTCGGTGACTGGCCTGGCGGCGATGCTCGCCCATGAGATCAAGAACCCGCTCTCCGGCATCCGCGGC
GCCGCGCAACTGCTCGAACTGTCCGCCTCCGACGAGGACCGCGCGTTGACCCGGCTGATCACCGACGAGACGGACCGCAT
CGTCTCGCTGGTCGATCGCATGGAGGTGTTTTCCGACGAGCGGCCGATCGATCGCTATCCCGTCAACATCCATGTCGTTC
TCGACCATGTGAAGGCGATCGCAAAGAACGGTTTTGCCAAGAAAATCAGGATATTGGAGGACTATGATCCATCATTGCCT
CCAGTGTTCGCCAACCGCGACCAGCTGATCCAGGTGTTCCTGAACCTGGTCAAGAACGCCGCCGAGGCGATCGGCACCGA
CCCGCATGGCGAGATCGTGCTGTCGACCGCCTTCCGGCCGGGCATTCGCGTATCGGTGCCGGGAACGCAGGACCGCGTGT
CGCTGCCCCTGGAGTTCTGCGTGCGCGACAATGGCCCCGGTGTCTCGGAGGATATCCTGCCGATCCTGTTTGATCCTTTC
ATCACCACCAAGCCGAACGGCTCGGGGCTGGGGCTTGCGCTGGTCGCCAAGATCGTCGGCGAGCATGGCGGCATCATCGA
ATGCGAATCGACCCCGCGAGGAACCACCTTCCGCATCCTGATGCCGGCCTGGAAGGAAACGCCGTTCGGCGCTGAAGAAG
ACGGTGAAGGAGACCGCAAATGA

Upstream 100 bases:

>100_bases
GAAAGGCCATCCTGACGGCGTTCGATCCGGCCCGCGTCATTGCCTTGCTGCGCAATGTGTTTTCGCGTGATCCGCAACCC
TTGAGCCTGCGGAGCGCCGC

Downstream 100 bases:

>100_bases
CCGCTCGCGGCAATATTCTCGTCGCCGATGACGATGCGGCGATCCGCACCGTGCTCAACCAGGCTCTCTCGCGCGTCGGG
CACGAGGTGCGCGTCACCTC

Product: nitrogen reguration protein nirB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNAIAGQGAEMVDAAQIVLNTIRRPVIMISEEGFITFANADAEDFFRSSATMLARNTLSKLVPFGSPLLTLVDQVRERRA
PVNEYRVDVSSPRLGIEKVVDLYVAPVPEFPGSVVVMFQERSMADKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRG
AAQLLELSASDEDRALTRLITDETDRIVSLVDRMEVFSDERPIDRYPVNIHVVLDHVKAIAKNGFAKKIRILEDYDPSLP
PVFANRDQLIQVFLNLVKNAAEAIGTDPHGEIVLSTAFRPGIRVSVPGTQDRVSLPLEFCVRDNGPGVSEDILPILFDPF
ITTKPNGSGLGLALVAKIVGEHGGIIECESTPRGTTFRILMPAWKETPFGAEEDGEGDRK

Sequences:

>Translated_380_residues
MNAIAGQGAEMVDAAQIVLNTIRRPVIMISEEGFITFANADAEDFFRSSATMLARNTLSKLVPFGSPLLTLVDQVRERRA
PVNEYRVDVSSPRLGIEKVVDLYVAPVPEFPGSVVVMFQERSMADKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRG
AAQLLELSASDEDRALTRLITDETDRIVSLVDRMEVFSDERPIDRYPVNIHVVLDHVKAIAKNGFAKKIRILEDYDPSLP
PVFANRDQLIQVFLNLVKNAAEAIGTDPHGEIVLSTAFRPGIRVSVPGTQDRVSLPLEFCVRDNGPGVSEDILPILFDPF
ITTKPNGSGLGLALVAKIVGEHGGIIECESTPRGTTFRILMPAWKETPFGAEEDGEGDRK
>Mature_380_residues
MNAIAGQGAEMVDAAQIVLNTIRRPVIMISEEGFITFANADAEDFFRSSATMLARNTLSKLVPFGSPLLTLVDQVRERRA
PVNEYRVDVSSPRLGIEKVVDLYVAPVPEFPGSVVVMFQERSMADKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRG
AAQLLELSASDEDRALTRLITDETDRIVSLVDRMEVFSDERPIDRYPVNIHVVLDHVKAIAKNGFAKKIRILEDYDPSLP
PVFANRDQLIQVFLNLVKNAAEAIGTDPHGEIVLSTAFRPGIRVSVPGTQDRVSLPLEFCVRDNGPGVSEDILPILFDPF
ITTKPNGSGLGLALVAKIVGEHGGIIECESTPRGTTFRILMPAWKETPFGAEEDGEGDRK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=354, Percent_Identity=30.225988700565, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=32.8888888888889, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788549, Length=353, Percent_Identity=27.4787535410765, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=28.1632653061224, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790567, Length=117, Percent_Identity=36.7521367521368, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41605; Mature: 41605

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIAGQGAEMVDAAQIVLNTIRRPVIMISEEGFITFANADAEDFFRSSATMLARNTLSK
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LVPFGSPLLTLVDQVRERRAPVNEYRVDVSSPRLGIEKVVDLYVAPVPEFPGSVVVMFQE
HCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
RSMADKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLELSASDEDRALTRLI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TDETDRIVSLVDRMEVFSDERPIDRYPVNIHVVLDHVKAIAKNGFAKKIRILEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
PVFANRDQLIQVFLNLVKNAAEAIGTDPHGEIVLSTAFRPGIRVSVPGTQDRVSLPLEFC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEE
VRDNGPGVSEDILPILFDPFITTKPNGSGLGLALVAKIVGEHGGIIECESTPRGTTFRIL
EECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
MPAWKETPFGAEEDGEGDRK
ECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAIAGQGAEMVDAAQIVLNTIRRPVIMISEEGFITFANADAEDFFRSSATMLARNTLSK
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LVPFGSPLLTLVDQVRERRAPVNEYRVDVSSPRLGIEKVVDLYVAPVPEFPGSVVVMFQE
HCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
RSMADKIDRQMTHRGAARSVTGLAAMLAHEIKNPLSGIRGAAQLLELSASDEDRALTRLI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TDETDRIVSLVDRMEVFSDERPIDRYPVNIHVVLDHVKAIAKNGFAKKIRILEDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
PVFANRDQLIQVFLNLVKNAAEAIGTDPHGEIVLSTAFRPGIRVSVPGTQDRVSLPLEFC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEE
VRDNGPGVSEDILPILFDPFITTKPNGSGLGLALVAKIVGEHGGIIECESTPRGTTFRIL
EECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
MPAWKETPFGAEEDGEGDRK
ECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]