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The map label for this gene is ntrC [H]

Identifier: 13470632

GI number: 13470632

Start: 283177

End: 284637

Strand: Direct

Name: ntrC [H]

Synonym: mlr0398

Alternate gene names: 13470632

Gene position: 283177-284637 (Clockwise)

Preceding gene: 13470631

Following gene: 13470633

Centisome position: 4.02

GC content: 64.27

Gene sequence:

>1461_bases
ATGACCGCTCGCGGCAATATTCTCGTCGCCGATGACGATGCGGCGATCCGCACCGTGCTCAACCAGGCTCTCTCGCGCGT
CGGGCACGAGGTGCGCGTCACCTCCAACGCCTCGACCTTGTGGCGCTGGGTGGCCGCGGGCGAGGGCGACCTTGTCATCA
CCGACGTCGTCATGCCGGATGAAAACGCCTTCGACATGCTACCGCGCATCAAGAAGGCGCGGCCGGAACTGCCGGTCATT
GTCATGAGCGCCCAGAACACGTTCATGACGGCGATCCGCGCATCCGAGACAGGCGCCTATGAATATCTCCCGAAACCGTT
CGACCTGACCGAACTGCTCAACATCGTCAACCGGGCGCTGTCTGAGCCCCGCCGGCCGAAGATCGATGCGCGGCCCGATG
AGCAGCCGGAAACGATGCCGCTGGTCGGCCGCTCGGCGGCCATGCAGGACATCTATCGCATGCTGGCGCGCATGATGCAG
ACCGACCTCACGGTGATGATCTCGGGCGAATCCGGCACCGGCAAGGAACTGGTGGCGCGTGCGCTGCACGAATATGGCCG
TCGTCGCGGCGGGCCGTTTGTCGCCATCAATATGGCGGCGATCCCGCGTGACCTGATCGAATCGGAACTGTTCGGCCATG
AGAAGGGTGCCTTCACCGGCGCGCAGAACCGCTCCACCGGCCGTTTCGAGCAGGCCGAGGGCGGCACGCTGTTCCTCGAC
GAGATCGGCGACATGCCGATGGAGGCACAGACGCGCCTGCTGCGCGTCCTGCAGCAGGGCGAGTACACGACCGTCGGCGG
ACGCACGCCGATCAAGACCGATGTGCGCATCGTCGCCGCCACCAACAAGGATCTGCGCACGCTGATCAACCAGGGACTGT
TCCGCGAGGATCTGTTCTATCGTCTCAATGTCGTTCCGCTGAGGCTGCCGGCATTGCGCGAGCGCTCCGAGGACGTGCCC
GATCTCGTGCGCCACTTCTTCAAGCTCGGCGAGATGGAAGGGCTGCAGACCAAGCGCATCTCGTCCGGCGGCATCGAACT
GATGAAGCGCTACCCGTGGCCGGGCAATGTGCGCGAACTGGAAAACCTGGTTCGCCGGCTGGCCGCTCTTTATTCGCAGG
ACGAGATTTCCGCCGAGATCATCGAGGCGGAGCTGAAGACCGGCGAGCGCCCCGTGGTGCCCGGCGGCGGCAACCTCATT
CCCGACGATCTCTCCATCGGCCAGGCAGTGGAGCACTTCCTGCAGCGCTATTTCGCCTCGTTTGCCGGCGAATTGCCGCC
CGCCGGACTCTACCAGCGCATACTGTCCGAGGTCGAATATCCGCTGGTCCTGGCCTCAATGACGGCAACCCGCGGCAACC
AGATCAAGGCTGCGGAACTGCTGGGGCTGAACCGCAACACGCTGCGCAAGAAGATTCGCGAACTGGGCGTCAACGTCTAT
AAATCGTCGCGGCCGGGCTGA

Upstream 100 bases:

>100_bases
AATGCGAATCGACCCCGCGAGGAACCACCTTCCGCATCCTGATGCCGGCCTGGAAGGAAACGCCGTTCGGCGCTGAAGAA
GACGGTGAAGGAGACCGCAA

Downstream 100 bases:

>100_bases
GCGACGACCCGGCGACGACTCTTTTTCAGGGGACTGAGCGGCGAAAGATTTCCGCCATTGTCACACTTGTTGCATAATCG
CCACAATGCGTTGCATAGAT

Product: nitrogen assimilation regulatory protein ntrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTARGNILVADDDAAIRTVLNQALSRVGHEVRVTSNASTLWRWVAAGEGDLVITDVVMPDENAFDMLPRIKKARPELPVI
VMSAQNTFMTAIRASETGAYEYLPKPFDLTELLNIVNRALSEPRRPKIDARPDEQPETMPLVGRSAAMQDIYRMLARMMQ
TDLTVMISGESGTGKELVARALHEYGRRRGGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLD
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRTLINQGLFREDLFYRLNVVPLRLPALRERSEDVP
DLVRHFFKLGEMEGLQTKRISSGGIELMKRYPWPGNVRELENLVRRLAALYSQDEISAEIIEAELKTGERPVVPGGGNLI
PDDLSIGQAVEHFLQRYFASFAGELPPAGLYQRILSEVEYPLVLASMTATRGNQIKAAELLGLNRNTLRKKIRELGVNVY
KSSRPG

Sequences:

>Translated_486_residues
MTARGNILVADDDAAIRTVLNQALSRVGHEVRVTSNASTLWRWVAAGEGDLVITDVVMPDENAFDMLPRIKKARPELPVI
VMSAQNTFMTAIRASETGAYEYLPKPFDLTELLNIVNRALSEPRRPKIDARPDEQPETMPLVGRSAAMQDIYRMLARMMQ
TDLTVMISGESGTGKELVARALHEYGRRRGGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLD
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRTLINQGLFREDLFYRLNVVPLRLPALRERSEDVP
DLVRHFFKLGEMEGLQTKRISSGGIELMKRYPWPGNVRELENLVRRLAALYSQDEISAEIIEAELKTGERPVVPGGGNLI
PDDLSIGQAVEHFLQRYFASFAGELPPAGLYQRILSEVEYPLVLASMTATRGNQIKAAELLGLNRNTLRKKIRELGVNVY
KSSRPG
>Mature_485_residues
TARGNILVADDDAAIRTVLNQALSRVGHEVRVTSNASTLWRWVAAGEGDLVITDVVMPDENAFDMLPRIKKARPELPVIV
MSAQNTFMTAIRASETGAYEYLPKPFDLTELLNIVNRALSEPRRPKIDARPDEQPETMPLVGRSAAMQDIYRMLARMMQT
DLTVMISGESGTGKELVARALHEYGRRRGGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRTLINQGLFREDLFYRLNVVPLRLPALRERSEDVPD
LVRHFFKLGEMEGLQTKRISSGGIELMKRYPWPGNVRELENLVRRLAALYSQDEISAEIIEAELKTGERPVVPGGGNLIP
DDLSIGQAVEHFLQRYFASFAGELPPAGLYQRILSEVEYPLVLASMTATRGNQIKAAELLGLNRNTLRKKIRELGVNVYK
SSRPG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=483, Percent_Identity=43.064182194617, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788550, Length=488, Percent_Identity=38.5245901639344, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=39.7027600849257, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788905, Length=391, Percent_Identity=39.1304347826087, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=51.5151515151515, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1789087, Length=439, Percent_Identity=37.129840546697, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789233, Length=262, Percent_Identity=47.7099236641221, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082152, Length=261, Percent_Identity=46.360153256705, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=44.047619047619, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI87081872, Length=343, Percent_Identity=38.7755102040816, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=39.647577092511, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1789828, Length=230, Percent_Identity=35.6521739130435, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=355, Percent_Identity=27.6056338028169, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=31.858407079646, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=28.7037037037037, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789661, Length=70, Percent_Identity=45.7142857142857, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=33.0188679245283, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54140; Mature: 54009

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARGNILVADDDAAIRTVLNQALSRVGHEVRVTSNASTLWRWVAAGEGDLVITDVVMPD
CCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEEEECCC
ENAFDMLPRIKKARPELPVIVMSAQNTFMTAIRASETGAYEYLPKPFDLTELLNIVNRAL
CCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHH
SEPRRPKIDARPDEQPETMPLVGRSAAMQDIYRMLARMMQTDLTVMISGESGTGKELVAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ALHEYGRRRGGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLD
HHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHH
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRTLINQGLFREDLFY
HHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHH
RLNVVPLRLPALRERSEDVPDLVRHFFKLGEMEGLQTKRISSGGIELMKRYPWPGNVREL
HEEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHCCCCCCCHHHH
ENLVRRLAALYSQDEISAEIIEAELKTGERPVVPGGGNLIPDDLSIGQAVEHFLQRYFAS
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAGELPPAGLYQRILSEVEYPLVLASMTATRGNQIKAAELLGLNRNTLRKKIRELGVNVY
HCCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCHHH
KSSRPG
CCCCCC
>Mature Secondary Structure 
TARGNILVADDDAAIRTVLNQALSRVGHEVRVTSNASTLWRWVAAGEGDLVITDVVMPD
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEEEECCC
ENAFDMLPRIKKARPELPVIVMSAQNTFMTAIRASETGAYEYLPKPFDLTELLNIVNRAL
CCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHH
SEPRRPKIDARPDEQPETMPLVGRSAAMQDIYRMLARMMQTDLTVMISGESGTGKELVAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
ALHEYGRRRGGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSTGRFEQAEGGTLFLD
HHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHH
EIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRIVAATNKDLRTLINQGLFREDLFY
HHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHH
RLNVVPLRLPALRERSEDVPDLVRHFFKLGEMEGLQTKRISSGGIELMKRYPWPGNVREL
HEEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHCCCCCCCHHHH
ENLVRRLAALYSQDEISAEIIEAELKTGERPVVPGGGNLIPDDLSIGQAVEHFLQRYFAS
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAGELPPAGLYQRILSEVEYPLVLASMTATRGNQIKAAELLGLNRNTLRKKIRELGVNVY
HCCCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCEEHHHHHCCCHHHHHHHHHHHCCHHH
KSSRPG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]