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The map label for this gene is dus [H]

Identifier: 13470630

GI number: 13470630

Start: 280968

End: 282041

Strand: Direct

Name: dus [H]

Synonym: mlr0396

Alternate gene names: 13470630

Gene position: 280968-282041 (Clockwise)

Preceding gene: 13470627

Following gene: 13470631

Centisome position: 3.99

GC content: 65.46

Gene sequence:

>1074_bases
ATGGCTAGAGAATGTGCAACGAATGGACATGCTTGGTTTTTGTGCATGGTTAACTCAGCCAGATTGGCCGCGCCTTTGAA
CGTCGGCGGCGTGGAAATCCGCAACCGCGTCTTCCTCGCGCCGATGTCGGGCATCACCGACGAGCCGTTCCGGCGGCGCG
CCCATGCGCACGGCGCGGGCCTGGTCGTGTCCGAAATGGTGGCAAGCGGCGAACTCGCCAAGGGTAGGGCCGGCTTCGAC
CTGCGCATACGGCATTCCGGCCTGCCCGTCCATATGGTGCAGCTGGCGGGCCGCGAAGCGATCCACATGGCCGAAGGCGC
ACGGATCGCCGCAGGCGAGGGCGCCGACATCATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTCACCGGTGGTTATG
CCGGTTCGGCATTGATGCTGGATCTCGACCATGCGCTGTCGCTGATCGAGGCCGTGGTCGGCGCGGTCAAGGTGCCGGTG
ACGGTCAAGATGCGGCTCGGCTGGGACGAAGGCGCCCTCAATGCGCCCATCCTCGCGCGCCGTGCCGAGCAGGCGGGCGT
CAAGATGGTGACGGTGCATGGCCGCACGCGCTGCCAGTTCTACCAGGGCAAGGCCGACTGGCGCGCCATCGCCCGCGTCA
AGGAAGCCGTGTCCATTCCAGTCGTTGCCAATGGCGATGTCTGTTCCCCGGCCGAGGCAGCTGCCATTCTCGACCAATCG
GGCGCCGATGCGGTGATGGTCGGTCGCGCGCATTACGGCGCGCCCTGGATCGCCGGTGGCATCGCGGCAGCGGCGGCAGG
CGAAGTGGCGGCCAACGTGCCCGAGAATCGCGCGGCTCTGGCAGACTATATCGTCGGCCACTACGAGGACATGCTGGCGC
TTTATGGCGTTGAGAGCGGTTTGCGCCAGGCGCGCAAACATCTGGGCTGGTATCTTGATCGCCATGCTGTTGGCGTCGCC
GACGATGACCGAAAGGCCATCCTGACGGCGTTCGATCCGGCCCGCGTCATTGCCTTGCTGCGCAATGTGTTTTCGCGTGA
TCCGCAACCCTTGAGCCTGCGGAGCGCCGCATGA

Upstream 100 bases:

>100_bases
GACGCGCGGCGCGCCCTGGTCACGGCAAATGCCTAAGGTCCGGAATTTTCCCAATTGCGCCTCAATGTGACGTCATTCCG
CGTTGCACATTGTAGCTGAT

Downstream 100 bases:

>100_bases
ACGCCATCGCTGGCCAGGGAGCTGAAATGGTGGACGCCGCCCAGATCGTCCTCAACACCATCCGCCGCCCGGTGATCATG
ATCAGCGAGGAAGGCTTCAT

Product: nitrogen reguration protein nifR3 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MARECATNGHAWFLCMVNSARLAAPLNVGGVEIRNRVFLAPMSGITDEPFRRRAHAHGAGLVVSEMVASGELAKGRAGFD
LRIRHSGLPVHMVQLAGREAIHMAEGARIAAGEGADIIDINMGCPAKKVTGGYAGSALMLDLDHALSLIEAVVGAVKVPV
TVKMRLGWDEGALNAPILARRAEQAGVKMVTVHGRTRCQFYQGKADWRAIARVKEAVSIPVVANGDVCSPAEAAAILDQS
GADAVMVGRAHYGAPWIAGGIAAAAAGEVAANVPENRAALADYIVGHYEDMLALYGVESGLRQARKHLGWYLDRHAVGVA
DDDRKAILTAFDPARVIALLRNVFSRDPQPLSLRSAA

Sequences:

>Translated_357_residues
MARECATNGHAWFLCMVNSARLAAPLNVGGVEIRNRVFLAPMSGITDEPFRRRAHAHGAGLVVSEMVASGELAKGRAGFD
LRIRHSGLPVHMVQLAGREAIHMAEGARIAAGEGADIIDINMGCPAKKVTGGYAGSALMLDLDHALSLIEAVVGAVKVPV
TVKMRLGWDEGALNAPILARRAEQAGVKMVTVHGRTRCQFYQGKADWRAIARVKEAVSIPVVANGDVCSPAEAAAILDQS
GADAVMVGRAHYGAPWIAGGIAAAAAGEVAANVPENRAALADYIVGHYEDMLALYGVESGLRQARKHLGWYLDRHAVGVA
DDDRKAILTAFDPARVIALLRNVFSRDPQPLSLRSAA
>Mature_356_residues
ARECATNGHAWFLCMVNSARLAAPLNVGGVEIRNRVFLAPMSGITDEPFRRRAHAHGAGLVVSEMVASGELAKGRAGFDL
RIRHSGLPVHMVQLAGREAIHMAEGARIAAGEGADIIDINMGCPAKKVTGGYAGSALMLDLDHALSLIEAVVGAVKVPVT
VKMRLGWDEGALNAPILARRAEQAGVKMVTVHGRTRCQFYQGKADWRAIARVKEAVSIPVVANGDVCSPAEAAAILDQSG
ADAVMVGRAHYGAPWIAGGIAAAAAGEVAANVPENRAALADYIVGHYEDMLALYGVESGLRQARKHLGWYLDRHAVGVAD
DDRKAILTAFDPARVIALLRNVFSRDPQPLSLRSAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=245, Percent_Identity=30.6122448979592, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI239788462, Length=251, Percent_Identity=30.2788844621514, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.3964757709251, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI40807366, Length=237, Percent_Identity=27.8481012658228, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=28.6274509803922, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789660, Length=293, Percent_Identity=44.7098976109215, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=33.4745762711864, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693211, Length=306, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=262, Percent_Identity=30.9160305343511, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=245, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=28.82096069869, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=227, Percent_Identity=29.0748898678414, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=235, Percent_Identity=28.0851063829787, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=311, Percent_Identity=26.3665594855305, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585320, Length=245, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24580595, Length=237, Percent_Identity=29.535864978903, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19920448, Length=237, Percent_Identity=29.535864978903, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=30, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=30.2631578947368, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37738; Mature: 37607

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARECATNGHAWFLCMVNSARLAAPLNVGGVEIRNRVFLAPMSGITDEPFRRRAHAHGAG
CCCCCCCCCCEEEEEEECCCCEECCCCCCCEEECCEEEEEECCCCCCCHHHHHHHCCCCC
LVVSEMVASGELAKGRAGFDLRIRHSGLPVHMVQLAGREAIHMAEGARIAAGEGADIIDI
HHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCHHEECCCCCEEECCCCCCEEEE
NMGCPAKKVTGGYAGSALMLDLDHALSLIEAVVGAVKVPVTVKMRLGWDEGALNAPILAR
CCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCCHHHH
RAEQAGVKMVTVHGRTRCQFYQGKADWRAIARVKEAVSIPVVANGDVCSPAEAAAILDQS
HHHHCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCC
GADAVMVGRAHYGAPWIAGGIAAAAAGEVAANVPENRAALADYIVGHYEDMLALYGVESG
CCCEEEEECCCCCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQARKHLGWYLDRHAVGVADDDRKAILTAFDPARVIALLRNVFSRDPQPLSLRSAA
HHHHHHHHHHHHHCCEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ARECATNGHAWFLCMVNSARLAAPLNVGGVEIRNRVFLAPMSGITDEPFRRRAHAHGAG
CCCCCCCCCEEEEEEECCCCEECCCCCCCEEECCEEEEEECCCCCCCHHHHHHHCCCCC
LVVSEMVASGELAKGRAGFDLRIRHSGLPVHMVQLAGREAIHMAEGARIAAGEGADIIDI
HHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCHHEECCCCCEEECCCCCCEEEE
NMGCPAKKVTGGYAGSALMLDLDHALSLIEAVVGAVKVPVTVKMRLGWDEGALNAPILAR
CCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCCCHHHH
RAEQAGVKMVTVHGRTRCQFYQGKADWRAIARVKEAVSIPVVANGDVCSPAEAAAILDQS
HHHHCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCC
GADAVMVGRAHYGAPWIAGGIAAAAAGEVAANVPENRAALADYIVGHYEDMLALYGVESG
CCCEEEEECCCCCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQARKHLGWYLDRHAVGVADDDRKAILTAFDPARVIALLRNVFSRDPQPLSLRSAA
HHHHHHHHHHHHHCCEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]