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The map label for this gene is fabI [H]

Identifier: 15602047

GI number: 15602047

Start: 205494

End: 206276

Strand: Reverse

Name: fabI [H]

Synonym: PM0182

Alternate gene names: 15602047

Gene position: 206276-205494 (Counterclockwise)

Preceding gene: 15602048

Following gene: 15602046

Centisome position: 9.14

GC content: 43.04

Gene sequence:

>783_bases
ATGGGTTTCTTAACAAACAAACGTATTTTAATTGCCGGTGTGGCAAGTAATCGTTCTATTGCTTACGGTATCGCACAAGC
AATGAAACGTGAAGGGGCAGAACTCGCTTTCACGTATCAAAATGATAAATTAAAAGGGCGTGTAGAAGAATTTGCTAAAG
AATTAGGTTCTGATATTGTGATTCCTTGTGATGTGGGCACGGATGAAAGCATCGAACAATGTTTTGCTGAATTAGCTAAA
CACTGGGAAAAATTTGATGGTTTTGTTCATTCTATCGCTTTTGCGCCAGGCGACCAATTAGACGGTGATTATGTGGATGC
AGTAAACCGCGAAGGTTTCCGTATTGCACACGATGTGAGTTCATATAGCTTTGTTGCGATGGCAAAAGCCGCGCGTGGCA
TGTTGAATCCGAACTCAGCCCTTTTAACACTTTCCTACTTAGGGGCAGAACGTGCAATTCCTAACTATAACGTAATGGGC
TTAGCCAAAGCCTCATTAGAAGCCAATACACGTTTTATGGCAGCAGCATTAGGTAAAGACGGTATCCGCGTAAATGCAAT
CTCTGCAGGACCAATTCGTACACTTGCCGCCTCTGGTATTAAAAACTTCAAGAAAATGCTCGCAAACTTCGAACAAACCG
CACCATTACGCCGTACTGTGACGATTGAAGATGTCGGTAACTCTGCAGCTTTCTTATGCTCAGATTTAAGTGCTGGTATC
ACAGGTGAAGTGGTCCATGTTGATGCCGGTTTCAGTATCGTAGCTATGGGTGAACTCGAATAA

Upstream 100 bases:

>100_bases
TAATCCTGCTTAAGAAAATAGGATATAAGGTCCGCTTTGTATTTATTCACATTTTCCCTTATACTAGCCAGAATTTTTAA
CATACGAAAAGGTATTGATT

Downstream 100 bases:

>100_bases
GTTTTACATGTTATATAAGAGCGGACATCAGTCCGCTTTCATTTTTTATGGATAGCCTCAATGTTCCAAAATAATCCCCT
CCTTTCACAATTAAAACAAC

Product: FabI

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFLTNKRILIAGVASNRSIAYGIAQAMKREGAELAFTYQNDKLKGRVEEFAKELGSDIVIPCDVGTDESIEQCFAELAK
HWEKFDGFVHSIAFAPGDQLDGDYVDAVNREGFRIAHDVSSYSFVAMAKAARGMLNPNSALLTLSYLGAERAIPNYNVMG
LAKASLEANTRFMAAALGKDGIRVNAISAGPIRTLAASGIKNFKKMLANFEQTAPLRRTVTIEDVGNSAAFLCSDLSAGI
TGEVVHVDAGFSIVAMGELE

Sequences:

>Translated_260_residues
MGFLTNKRILIAGVASNRSIAYGIAQAMKREGAELAFTYQNDKLKGRVEEFAKELGSDIVIPCDVGTDESIEQCFAELAK
HWEKFDGFVHSIAFAPGDQLDGDYVDAVNREGFRIAHDVSSYSFVAMAKAARGMLNPNSALLTLSYLGAERAIPNYNVMG
LAKASLEANTRFMAAALGKDGIRVNAISAGPIRTLAASGIKNFKKMLANFEQTAPLRRTVTIEDVGNSAAFLCSDLSAGI
TGEVVHVDAGFSIVAMGELE
>Mature_259_residues
GFLTNKRILIAGVASNRSIAYGIAQAMKREGAELAFTYQNDKLKGRVEEFAKELGSDIVIPCDVGTDESIEQCFAELAKH
WEKFDGFVHSIAFAPGDQLDGDYVDAVNREGFRIAHDVSSYSFVAMAKAARGMLNPNSALLTLSYLGAERAIPNYNVMGL
AKASLEANTRFMAAALGKDGIRVNAISAGPIRTLAASGIKNFKKMLANFEQTAPLRRTVTIEDVGNSAAFLCSDLSAGIT
GEVVHVDAGFSIVAMGELE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=240, Percent_Identity=27.5, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=24.6031746031746, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787545, Length=260, Percent_Identity=81.9230769230769, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=25.6916996047431, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=262, Percent_Identity=28.2442748091603, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27851; Mature: 27720

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTNKRILIAGVASNRSIAYGIAQAMKREGAELAFTYQNDKLKGRVEEFAKELGSDIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEE
IPCDVGTDESIEQCFAELAKHWEKFDGFVHSIAFAPGDQLDGDYVDAVNREGFRIAHDVS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCEEEECCC
SYSFVAMAKAARGMLNPNSALLTLSYLGAERAIPNYNVMGLAKASLEANTRFMAAALGKD
CHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCEEEEEEHHHCCCCEEEEEECCCC
GIRVNAISAGPIRTLAASGIKNFKKMLANFEQTAPLRRTVTIEDVGNSAAFLCSDLSAGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCC
TGEVVHVDAGFSIVAMGELE
CCEEEEEECCCEEEEECCCC
>Mature Secondary Structure 
GFLTNKRILIAGVASNRSIAYGIAQAMKREGAELAFTYQNDKLKGRVEEFAKELGSDIV
CCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEE
IPCDVGTDESIEQCFAELAKHWEKFDGFVHSIAFAPGDQLDGDYVDAVNREGFRIAHDVS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCEEEECCC
SYSFVAMAKAARGMLNPNSALLTLSYLGAERAIPNYNVMGLAKASLEANTRFMAAALGKD
CHHHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCCCEEEEEEHHHCCCCEEEEEECCCC
GIRVNAISAGPIRTLAASGIKNFKKMLANFEQTAPLRRTVTIEDVGNSAAFLCSDLSAGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCC
TGEVVHVDAGFSIVAMGELE
CCEEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]