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The map label for this gene is rluB

Identifier: 30024033

GI number: 30024033

Start: 1307979

End: 1308698

Strand: Reverse

Name: rluB

Synonym: L107724

Alternate gene names: 30024033

Gene position: 1308698-1307979 (Counterclockwise)

Preceding gene: 30024034

Following gene: 30024032

Centisome position: 55.32

GC content: 37.64

Gene sequence:

>720_bases
ATGAGAATCAATAAATATTTGGCCCATGCAGGTGTGGCAAGCAGACGTAAAGCAGAAGAACTAATTTTAGCGGGAAAAGT
AACGGTAAATAATGTTCCAATGACAAACTTGGGTTATCAAGTTTCAACTGGAGATATCGTTGAAGTTAATGGAGTAGCAG
TTTATAACGAAGAACCCGTTTATTATCTTCTAAATAAACCAAGAGGCTATATTTCTTCAGTTAGCGATGACAAAGGACGT
CAAACGGTAATGGATTTAATGCCTCAAGTTAAAGAACGGATTTACCCTGTGGGACGTTTGGACTGGGACACAAGCGGTTT
GCTTTTGCTTACAAACGACGGTGAGTTTACGAATATGATGACTCACCCACGTCATGGCGTTGATAAGGTATATGTTGCTA
AGGTAGAAGGCCAAGCGAACAAAGAAAACTTGCGTCCTTTAACTTTAGGTATGACTATTGAAGGTAAAAAAGTTAGTCCC
GCCCGTTATGAGATTATCAAACAAGAGAAAACAAAAAATCATTCGATTGTTAGTTTAACTATCCATGAAGGACAAAATCA
TCAAGTTAAAAAAATGTTTGCGGCTGCAGGACTTCCAGTACAAAAATTGAGTCGTATTCAATATGGATCTCTTACATTGG
ATGGACTTCCAACAGGACAATTTCGTCGTTTGAGTAAAAAAGAAGTTTCTCAATTGATTAATGCAGCCCAAGGAAATTAA

Upstream 100 bases:

>100_bases
TTAGACTATATTGGGATTAATCATTTAGATGAATTACCTGAAATAGATGAGTCAAGATTTATCGCAGAAGAACAAACCTT
ATTTAACGAAAGTGAAGAAA

Downstream 100 bases:

>100_bases
GTGATTATTATAAAAGACAATTCTGGTAAAGAGTTGTTTTTTTATTTCTTAAGCAAATCTTATTCTTGATAAGCAAACTA
TTTTTGTGCTATAATAAAAA

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDIVEVNGVAVYNEEPVYYLLNKPRGYISSVSDDKGR
QTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPRHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSP
ARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGSLTLDGLPTGQFRRLSKKEVSQLINAAQGN

Sequences:

>Translated_239_residues
MRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDIVEVNGVAVYNEEPVYYLLNKPRGYISSVSDDKGR
QTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPRHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSP
ARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGSLTLDGLPTGQFRRLSKKEVSQLINAAQGN
>Mature_239_residues
MRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDIVEVNGVAVYNEEPVYYLLNKPRGYISSVSDDKGR
QTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMMTHPRHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSP
ARYEIIKQEKTKNHSIVSLTIHEGQNHQVKKMFAAAGLPVQKLSRIQYGSLTLDGLPTGQFRRLSKKEVSQLINAAQGN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=33.6134453781513, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=34.3347639484979, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=32.2033898305085, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=32.3863636363636, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26604; Mature: 26604

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDIVEVNGVAVYNEEPV
CCCHHHHHHCCHHHHHHHHHEEEECEEEECCCCCCCCCEEECCCCEEEECCEEEECCCCE
YYLLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMM
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
THPRHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSPARYEIIKQEKTKNHSIVSLT
CCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
IHEGQNHQVKKMFAAAGLPVQKLSRIQYGSLTLDGLPTGQFRRLSKKEVSQLINAAQGN
EECCCCCHHHHHHHHCCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRINKYLAHAGVASRRKAEELILAGKVTVNNVPMTNLGYQVSTGDIVEVNGVAVYNEEPV
CCCHHHHHHCCHHHHHHHHHEEEECEEEECCCCCCCCCEEECCCCEEEECCEEEECCCCE
YYLLNKPRGYISSVSDDKGRQTVMDLMPQVKERIYPVGRLDWDTSGLLLLTNDGEFTNMM
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
THPRHGVDKVYVAKVEGQANKENLRPLTLGMTIEGKKVSPARYEIIKQEKTKNHSIVSLT
CCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
IHEGQNHQVKKMFAAAGLPVQKLSRIQYGSLTLDGLPTGQFRRLSKKEVSQLINAAQGN
EECCCCCHHHHHHHHCCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]