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The map label for this gene is priA

Identifier: 15673879

GI number: 15673879

Start: 1963387

End: 1965726

Strand: Reverse

Name: priA

Synonym: L0292

Alternate gene names: 15673879

Gene position: 1965726-1963387 (Counterclockwise)

Preceding gene: 15673880

Following gene: 15673878

Centisome position: 83.1

GC content: 36.24

Gene sequence:

>2340_bases
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAGATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAAGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Upstream 100 bases:

>100_bases
AAGTTACTGACAGCTTTGTCAGTAAGAAGCGGATAAGTCAAAAGCAGGAAAAATGCTTTTCTGTTATAATTAAAGATAAG
AAAACGGAGGTCAAAAATAA

Downstream 100 bases:

>100_bases
AAATTTCCACAGGTGGAAAAGTTAAGAATTTGAAAACAAAAGAAAGTTGGTGTGGAAAATGCAGAAAATAGAACTTTTTC
AACAAACAATTGACGCATGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Sequences:

>Translated_779_residues
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV
>Mature_779_residues
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=796, Percent_Identity=34.0452261306533, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 88930; Mature: 88930

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEEL
CCHHHEEEECCCCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCC
EPSVNLKGISELLDVEPVLTDEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKS
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
TDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENKKTEKFLSIPDIEKLAEFEIE
CCCCCEEHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
KRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
HHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCC
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPE
CCCHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEEEC
ISLTPQITNRFIARFGDKVAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENL
CCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHCCHHHC
GIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVLGSATPSLETRARAMRGVYEL
CEEEEECCCCCCCCCCCCCCEEHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
LLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
HHHHHCCCCCEECCCEEEEECHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGT
HHHHHHHCCCEEECCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCH
QKVEEELREIMPEARVLRMDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNV
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCE
TLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKKGEVLIQTFNPHHYAIEMTKA
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCH
QDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV
CCCCCHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEEL
CCHHHEEEECCCCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCC
EPSVNLKGISELLDVEPVLTDEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKS
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
TDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENKKTEKFLSIPDIEKLAEFEIE
CCCCCEEHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHH
KRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
HHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCC
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPE
CCCHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEEEC
ISLTPQITNRFIARFGDKVAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENL
CCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHCCHHHC
GIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVLGSATPSLETRARAMRGVYEL
CEEEEECCCCCCCCCCCCCCEEHHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
LLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
HHHHHCCCCCEECCCEEEEECHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGT
HHHHHHHCCCEEECCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCH
QKVEEELREIMPEARVLRMDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNV
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCE
TLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKKGEVLIQTFNPHHYAIEMTKA
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEECCH
QDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV
CCCCCHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]