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The map label for this gene is rnhB

Identifier: 15673275

GI number: 15673275

Start: 1328294

End: 1329070

Strand: Reverse

Name: rnhB

Synonym: L0320

Alternate gene names: 15673275

Gene position: 1329070-1328294 (Counterclockwise)

Preceding gene: 15673276

Following gene: 15673274

Centisome position: 56.18

GC content: 36.04

Gene sequence:

>777_bases
ATGGGACAAACCATTAAAGAAATTAAAGCGACCTTAGCCAAACTGACAGACTTGTCAGCAAAAGAATTCTTAGAGTATGA
AGCAGATGAACGGGCTGGGGTTCAAGCAGCACTTAAAAGTAGGAAAAAACAAATTTTAGCAGAGTTAGCAGAAGAGGCTC
GTTTGGAACAAATGTTGGAATTTGAGAAAGAACTTTACGGTCAAGAAATTCAATTAATTGCAGGTATTGATGAAGTTGGT
CGTGGACCACTTGCTGGTCCTGTAGTGACTGCGGCAGTCATTTTACCTAAAAATTGTAAAATTCGGGGTTTAAATGATAG
TAAAAAAGTGCCAAAATCAAAACATCATGCTATTCTATCTGAAATTCAAGAAAAAGCTTTAGCAATAGGAATTGGGATTG
TTGATGCGGAAAAAATTGATGAAGTAAATATTTATGAGGCAACAAAAATTGCAATGATTCAGGCAGTATCAAAATTATCT
GTTAAACCTGAGCATCTCTTAATTGATGCTATGGTTTTGGATTTTCCTATTGCACAGACAAAAATTATTCACGGAGATGC
TCGTTCAGCCTCGATTGCGGCAGCTTCAATTGTTGCTAAAGTAACTCGTGATGAAATGATGAAAGAGTTTGCTCTTGAAT
TTCCTGAGTATGATTTTGAACATAATGCAGGTTATGGAACAGCAAAACATCTTGAAGCTCTAACAAAATATGGTATCACA
AGAATTCATCGCAAGTCCTATGAGCCGATTAAAACAATGGTCAATTTTAAATCGTAG

Upstream 100 bases:

>100_bases
TGTGATTACTGACAGATTTTCAGTATCAATAACATAAATAATATTATGTAAAACTACTGACAGAAAAGCTGTCAGTAATT
TTTGAGAGTTGGTGAGTAAA

Downstream 100 bases:

>100_bases
ATTCTGTCAGTAAAAATATGTAAAACGTGAACGTTATCTGTTCACGTTTTTTTGCTGACAGGTCTGTCAGTAATTATTTA
GACTATTCTTTTTTAATAAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGQTIKEIKATLAKLTDLSAKEFLEYEADERAGVQAALKSRKKQILAELAEEARLEQMLEFEKELYGQEIQLIAGIDEVG
RGPLAGPVVTAAVILPKNCKIRGLNDSKKVPKSKHHAILSEIQEKALAIGIGIVDAEKIDEVNIYEATKIAMIQAVSKLS
VKPEHLLIDAMVLDFPIAQTKIIHGDARSASIAAASIVAKVTRDEMMKEFALEFPEYDFEHNAGYGTAKHLEALTKYGIT
RIHRKSYEPIKTMVNFKS

Sequences:

>Translated_258_residues
MGQTIKEIKATLAKLTDLSAKEFLEYEADERAGVQAALKSRKKQILAELAEEARLEQMLEFEKELYGQEIQLIAGIDEVG
RGPLAGPVVTAAVILPKNCKIRGLNDSKKVPKSKHHAILSEIQEKALAIGIGIVDAEKIDEVNIYEATKIAMIQAVSKLS
VKPEHLLIDAMVLDFPIAQTKIIHGDARSASIAAASIVAKVTRDEMMKEFALEFPEYDFEHNAGYGTAKHLEALTKYGIT
RIHRKSYEPIKTMVNFKS
>Mature_257_residues
GQTIKEIKATLAKLTDLSAKEFLEYEADERAGVQAALKSRKKQILAELAEEARLEQMLEFEKELYGQEIQLIAGIDEVGR
GPLAGPVVTAAVILPKNCKIRGLNDSKKVPKSKHHAILSEIQEKALAIGIGIVDAEKIDEVNIYEATKIAMIQAVSKLSV
KPEHLLIDAMVLDFPIAQTKIIHGDARSASIAAASIVAKVTRDEMMKEFALEFPEYDFEHNAGYGTAKHLEALTKYGITR
IHRKSYEPIKTMVNFKS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=202, Percent_Identity=46.5346534653465, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_LACLA (Q9CG17)

Other databases:

- EMBL:   AE005176
- PIR:   E86786
- RefSeq:   NP_267449.1
- ProteinModelPortal:   Q9CG17
- SMR:   Q9CG17
- GeneID:   1114942
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0320
- NMPDR:   fig|272623.1.peg.1328
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   LLAC272623:L0320-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28526; Mature: 28394

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTIKEIKATLAKLTDLSAKEFLEYEADERAGVQAALKSRKKQILAELAEEARLEQMLE
CCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKELYGQEIQLIAGIDEVGRGPLAGPVVTAAVILPKNCKIRGLNDSKKVPKSKHHAILS
HHHHHCCCHHHEEECHHHHCCCCCCHHHHHHHHEECCCCEEECCCCHHCCCCHHHHHHHH
EIQEKALAIGIGIVDAEKIDEVNIYEATKIAMIQAVSKLSVKPEHLLIDAMVLDFPIAQT
HHHHHHHHHHCCEECHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
KIIHGDARSASIAAASIVAKVTRDEMMKEFALEFPEYDFEHNAGYGTAKHLEALTKYGIT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
RIHRKSYEPIKTMVNFKS
HHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
GQTIKEIKATLAKLTDLSAKEFLEYEADERAGVQAALKSRKKQILAELAEEARLEQMLE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKELYGQEIQLIAGIDEVGRGPLAGPVVTAAVILPKNCKIRGLNDSKKVPKSKHHAILS
HHHHHCCCHHHEEECHHHHCCCCCCHHHHHHHHEECCCCEEECCCCHHCCCCHHHHHHHH
EIQEKALAIGIGIVDAEKIDEVNIYEATKIAMIQAVSKLSVKPEHLLIDAMVLDFPIAQT
HHHHHHHHHHCCEECHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
KIIHGDARSASIAAASIVAKVTRDEMMKEFALEFPEYDFEHNAGYGTAKHLEALTKYGIT
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
RIHRKSYEPIKTMVNFKS
HHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471