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The map label for this gene is bcaT

Identifier: 15673270

GI number: 15673270

Start: 1321794

End: 1322816

Strand: Reverse

Name: bcaT

Synonym: L0086

Alternate gene names: 15673270

Gene position: 1322816-1321794 (Counterclockwise)

Preceding gene: 15673272

Following gene: 15673268

Centisome position: 55.92

GC content: 39.98

Gene sequence:

>1023_bases
ATGGCAATTAATTTAGACTGGGAAAATTTAGGATTCAGCTATCGGAACTTACCTTTTCGTTATATCGCTCGTTTTAAAGA
TGGAAAATGGAGTGCTGGAGAACTAACAGGAGATAATCAACTTCATATTAGTGAATCATCACCTGCTTTGCATTATGGTC
AACAAGGTTTTGAAGGATTAAAAGCCTATCGAACAAAGGATGGTTCAATCCAACTTTTCCGTCCTGACCAAAATGCTGCT
CGTTTGCAAAATACGGCGCGTCGACTTTGCATGGCAGAAGTTCCAACTGAAATGTTTATTGATGCAGTTAAACAAGTGGT
GAAAGCAAACGAAGATTTTGTGCCTCCTTACGGAACGGGTGCAACGCTCTATCTCCGTCCACTTTTGATTGGGGTTGGTG
ACGTTATTGGGGTGAAACCTGCTGATGAATATATTTTCACCGTTTTTGCTATGCCGGTTGGTTCTTATTTTAAAGGCGGA
TTGGCTCCTTCAAAATTTGTAATTTCAAGAGATTATGATAGGGCAGCTCCACTTGGTACAGGTGGTGCCAAAGTTGGAGG
AAATTATGCAGCTTCTTTACAAGCAGAAGTTGGTGCCAAAGCTTCAGGCTATGCAGATGCAATTTATCTTGACCCAAGCA
CACATACTAAAATTGAAGAAGTCGGGGCAGCAAATTTCTTTGGAATTACAGCCGATAATGAATTTATCACACCATTGAGT
CCATCAATCTTACCTTCAATTACTAAATATTCTCTTCTTTATTTAGCTGAACATCGTTTGGGACTCAAAGCGATTGAGGG
TGAAGTTTATGCCAAAGATTTAGGTAAATTTGTTGAAGCAGGAGCTTGTGGCACAGCGGCAATTATCTCTCCAATTGGTC
GTATTGACGATGGAGAAGATTCTTACATTTTCCATTCAGAAACAGAAGTAGGACCAACGGTTAAACGTTTATATGATGAG
TTGGTTGGCATTCAGTTTGGTGATGTTGAAGCACCAGAAGGCTGGATAGTTAAAGTTGATTAA

Upstream 100 bases:

>100_bases
AAAAATTGTTTGAACTTTTACTGAATTTCATGAATATTTTCTTGTTTTATGTTACAGAAAATAATATAATCTATTATAAC
AAAAAAATTTGGAGGATGTT

Downstream 100 bases:

>100_bases
TTTAAAAAGCTAAAGAATACTCTTTAGCTTTTTTATTTTGAAATAAATAATTTATCTTTTTTAATCCATTCTTGATCCTC
TGTTCGAATTGGACGTATCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAINLDWENLGFSYRNLPFRYIARFKDGKWSAGELTGDNQLHISESSPALHYGQQGFEGLKAYRTKDGSIQLFRPDQNAA
RLQNTARRLCMAEVPTEMFIDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGDVIGVKPADEYIFTVFAMPVGSYFKGG
LAPSKFVISRDYDRAAPLGTGGAKVGGNYAASLQAEVGAKASGYADAIYLDPSTHTKIEEVGAANFFGITADNEFITPLS
PSILPSITKYSLLYLAEHRLGLKAIEGEVYAKDLGKFVEAGACGTAAIISPIGRIDDGEDSYIFHSETEVGPTVKRLYDE
LVGIQFGDVEAPEGWIVKVD

Sequences:

>Translated_340_residues
MAINLDWENLGFSYRNLPFRYIARFKDGKWSAGELTGDNQLHISESSPALHYGQQGFEGLKAYRTKDGSIQLFRPDQNAA
RLQNTARRLCMAEVPTEMFIDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGDVIGVKPADEYIFTVFAMPVGSYFKGG
LAPSKFVISRDYDRAAPLGTGGAKVGGNYAASLQAEVGAKASGYADAIYLDPSTHTKIEEVGAANFFGITADNEFITPLS
PSILPSITKYSLLYLAEHRLGLKAIEGEVYAKDLGKFVEAGACGTAAIISPIGRIDDGEDSYIFHSETEVGPTVKRLYDE
LVGIQFGDVEAPEGWIVKVD
>Mature_339_residues
AINLDWENLGFSYRNLPFRYIARFKDGKWSAGELTGDNQLHISESSPALHYGQQGFEGLKAYRTKDGSIQLFRPDQNAAR
LQNTARRLCMAEVPTEMFIDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGDVIGVKPADEYIFTVFAMPVGSYFKGGL
APSKFVISRDYDRAAPLGTGGAKVGGNYAASLQAEVGAKASGYADAIYLDPSTHTKIEEVGAANFFGITADNEFITPLSP
SILPSITKYSLLYLAEHRLGLKAIEGEVYAKDLGKFVEAGACGTAAIISPIGRIDDGEDSYIFHSETEVGPTVKRLYDEL
VGIQFGDVEAPEGWIVKVD

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=316, Percent_Identity=33.8607594936709, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI38176287, Length=316, Percent_Identity=33.8607594936709, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI296010904, Length=316, Percent_Identity=33.8607594936709, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI50658084, Length=319, Percent_Identity=30.4075235109718, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI258614015, Length=286, Percent_Identity=31.4685314685315, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI296010902, Length=249, Percent_Identity=33.7349397590361, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI296010900, Length=249, Percent_Identity=33.7349397590361, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI48994963, Length=318, Percent_Identity=30.5031446540881, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17568601, Length=326, Percent_Identity=30.3680981595092, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17565728, Length=329, Percent_Identity=29.1793313069909, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322608, Length=318, Percent_Identity=33.9622641509434, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322002, Length=324, Percent_Identity=32.7160493827161, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24641779, Length=326, Percent_Identity=33.4355828220859, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 36900; Mature: 36769

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINLDWENLGFSYRNLPFRYIARFKDGKWSAGELTGDNQLHISESSPALHYGQQGFEGL
CEEECCHHHCCCCHHCCCHHHHEEECCCCCCCCCCCCCCEEEEECCCCCHHCCHHHHHHH
KAYRTKDGSIQLFRPDQNAARLQNTARRLCMAEVPTEMFIDAVKQVVKANEDFVPPYGTG
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPLLIGVGDVIGVKPADEYIFTVFAMPVGSYFKGGLAPSKFVISRDYDRAAPLGT
CCEEEHHHHHHCCCCCCCCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCCCCCCCC
GGAKVGGNYAASLQAEVGAKASGYADAIYLDPSTHTKIEEVGAANFFGITADNEFITPLS
CCCCCCCCEEEEEEHHCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCCC
PSILPSITKYSLLYLAEHRLGLKAIEGEVYAKDLGKFVEAGACGTAAIISPIGRIDDGED
CHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
SYIFHSETEVGPTVKRLYDELVGIQFGDVEAPEGWIVKVD
CEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC
>Mature Secondary Structure 
AINLDWENLGFSYRNLPFRYIARFKDGKWSAGELTGDNQLHISESSPALHYGQQGFEGL
EEECCHHHCCCCHHCCCHHHHEEECCCCCCCCCCCCCCEEEEECCCCCHHCCHHHHHHH
KAYRTKDGSIQLFRPDQNAARLQNTARRLCMAEVPTEMFIDAVKQVVKANEDFVPPYGTG
HHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPLLIGVGDVIGVKPADEYIFTVFAMPVGSYFKGGLAPSKFVISRDYDRAAPLGT
CCEEEHHHHHHCCCCCCCCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCCCCCCCC
GGAKVGGNYAASLQAEVGAKASGYADAIYLDPSTHTKIEEVGAANFFGITADNEFITPLS
CCCCCCCCEEEEEEHHCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEEEECCCCEECCCC
PSILPSITKYSLLYLAEHRLGLKAIEGEVYAKDLGKFVEAGACGTAAIISPIGRIDDGED
CHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
SYIFHSETEVGPTVKRLYDELVGIQFGDVEAPEGWIVKVD
CEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]