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The map label for this gene is gadB

Identifier: 15673272

GI number: 15673272

Start: 1323836

End: 1325236

Strand: Reverse

Name: gadB

Synonym: L123581

Alternate gene names: 15673272

Gene position: 1325236-1323836 (Counterclockwise)

Preceding gene: 15673273

Following gene: 15673270

Centisome position: 56.02

GC content: 34.9

Gene sequence:

>1401_bases
ATGTTATACGGAAAAGAAAATCGCGATGAAGCAGAGTTCTTGGAACCAATTTTTGGTTCAGAAAGTGAACAAGTGGATTT
GCCTAAATATAAATTAGCTCAACAATCAATTGAACCTCGAGTGGCCTATCAGTTAGTTCAAGATGAAATGTTAGATGAAG
GGAACGCTCGTTTAAATTTAGCCACATTCTGTCAAACTTATATGGAACCTGAAGCAGTCAAACTAATGAGTCAAACCTTG
GAAAAAAATGCAATTGATAAATCGGAATATCCAAGAACAACTGAAATTGAGAACCGTTGCGTCAACATGATCGCTGACCT
TTGGAATGCGAGTGAAAAAGAAAAATTTATGGGGACTTCAACGATTGGTTCTTCAGAAGCTTGTATGCTTGGTGGAATGG
CCATGAAGTTTTCTTGGCGCAAGCGAGCAGAAAAATTGGGATTAGATATTAATGCGAAAAAGCCAAACTTAGTTATTTCA
TCTGGTTATCAAGTTTGCTGGGAAAAATTCTGTATTTATTGGGATATTGAAATGCGAGAAGTGCCAATGGATAAAGAACA
TATGTCAATCAATTTGGACAAGGTGATGGATTATGTTGATGAGTACACGATTGGTGTAGTTGGTATTATGGGGATTACTT
ATACTGGTCGTTATGATGATATCAAAGCTTTGGATAATTTAATTGAAGAATATAATAAACAGACAGACTATAAAGTTTAT
ATTCACGTAGATGCTGCTTCAGGAGGACTTTATGCTCCCTTTGTTGAGCCAGAACTTGAGTGGGATTTCCGTTTGAAAAA
TGTCATTTCAATCAATACCTCAGGCCATAAATATGGTTTAGTTTATCCTGGTGTAGGTTGGGTTTTGTGGCGTGACAAAA
AATATTTACCAGAAGAATTAATTTTTAAAGTAAGTTATCTTGGAGGAGAACTACCAACGATGGCCATTAATTTTTCTCAT
AGTGCCTCTCAATTAATTGGTCAATATTATAATTTTGTACGTTATGGATTTGATGGATATAAAGCTATTCATGAGAGAAC
ACATAAAGTAGCCATGTTTTTAGCAAAAGAAATTGAAAAAACTGGAATGTTTGAAATTATGAACGATGGGTCACAATTGC
CAATTGTCTGCTATAAATTAAAAGAAGATTCAAATCGAGGTTGGAATCTTTATGATTTGGCGGACCGTTTATTAATGAAG
GGATGGCAAGTGCCTGCTTATCCACTTCCCAAAAATTTGGAAAATGAAATCATTCAACGTTTAGTGATTCGAGCAGATTT
TGGGATGAATATGGCATTTAACTATGTTCAAGATATGCAAGAAGCAATTGAGGCTTTAAATAAGGCTCATATTCTATATC
ATGAAGAGCCTGAAAATAAAACATATGGATTTACTCACTAA

Upstream 100 bases:

>100_bases
GATGTGAACCCCGCGATTTATCCAGCAGCTCGTGGTGAGCATCATATTATTAAAAAAGAAGAACATATCTTAAAACATTA
AAAAATTGGAGGATGTACCT

Downstream 100 bases:

>100_bases
AAAAACCGATATTCATCTTAAGAAAAATCAAAAGCGATATAGTTAATCTATATCGCTTTTATTTTGATGATAAATTTTGT
TTTTTTCTATTTAGTCATAA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCIYWDIEMREVPMDKEHMSINLDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEKTGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIEALNKAHILYHEEPENKTYGFTH

Sequences:

>Translated_466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCIYWDIEMREVPMDKEHMSINLDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEKTGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIEALNKAHILYHEEPENKTYGFTH
>Mature_466_residues
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNLATFCQTYMEPEAVKLMSQTL
EKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVIS
SGYQVCWEKFCIYWDIEMREVPMDKEHMSINLDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYLGGELPTMAINFSH
SASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEKTGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMK
GWQVPAYPLPKNLENEIIQRLVIRADFGMNMAFNYVQDMQEAIEALNKAHILYHEEPENKTYGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family

Homologues:

Organism=Homo sapiens, GI31982936, Length=387, Percent_Identity=23.7726098191214, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787769, Length=443, Percent_Identity=44.6952595936795, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1789934, Length=443, Percent_Identity=44.4695259593679, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17557272, Length=366, Percent_Identity=24.8633879781421, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17543922, Length=387, Percent_Identity=24.5478036175711, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI25148342, Length=320, Percent_Identity=22.1875, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323905, Length=466, Percent_Identity=35.8369098712446, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6320500, Length=341, Percent_Identity=24.3401759530792, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355963, Length=402, Percent_Identity=24.3781094527363, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24654344, Length=402, Percent_Identity=24.3781094527363, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCE_LACLA (Q9CG20)

Other databases:

- EMBL:   AB010789
- EMBL:   AE005176
- PIR:   B86786
- RefSeq:   NP_267446.1
- ProteinModelPortal:   Q9CG20
- GeneID:   1114939
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L123581
- NMPDR:   fig|272623.1.peg.1325
- HOGENOM:   HBG365574
- OMA:   YPRTAEL
- ProtClustDB:   CLSK876864
- BioCyc:   LLAC272623:L123581-MONOMER
- BRENDA:   4.1.1.15
- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- Gene3D:   G3DSA:3.40.640.10
- PANTHER:   PTHR11999:SF1
- PANTHER:   PTHR11999
- TIGRFAMs:   TIGR01788

Pfam domain/function: PF00282 Pyridoxal_deC; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.1.15

Molecular weight: Translated: 53927; Mature: 53927

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00392 DDC_GAD_HDC_YDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEH
ATFCQTYMEPEAVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
TIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCIYWDIEMRE
CCCCCCHHHHCCCCEEHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHCEEEEEEEEHEE
VPMDKEHMSINLDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
CCCCHHHHEEEHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEEL
EEEEECCCCEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHH
IFKVSYLGGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQR
CCCHHEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LVIRADFGMNMAFNYVQDMQEAIEALNKAHILYHEEPENKTYGFTH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLYGKENRDEAEFLEPIFGSESEQVDLPKYKLAQQSIEPRVAYQLVQDEMLDEGNARLNL
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEH
ATFCQTYMEPEAVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
TIGSSEACMLGGMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCIYWDIEMRE
CCCCCCHHHHCCCCEEHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHCEEEEEEEEHEE
VPMDKEHMSINLDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVY
CCCCHHHHEEEHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
IHVDAASGGLYAPFVEPELEWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEEL
EEEEECCCCEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHH
IFKVSYLGGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
TGMFEIMNDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQR
CCCHHEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LVIRADFGMNMAFNYVQDMQEAIEALNKAHILYHEEPENKTYGFTH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471