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The map label for this gene is gltD

Identifier: 15673267

GI number: 15673267

Start: 1315082

End: 1316521

Strand: Reverse

Name: gltD

Synonym: L114827

Alternate gene names: 15673267

Gene position: 1316521-1315082 (Counterclockwise)

Preceding gene: 15673268

Following gene: 15673266

Centisome position: 55.65

GC content: 39.93

Gene sequence:

>1440_bases
ATGGCTGATCCAAATGGATTTTTAAATTATCCAAGAAATGATAATCCTTACCGTGAATTAGCGGAGCGGATAAAGGATTT
TGCTGAACTGCAAGTTCCCTTATCTACTGAAGAACGACAAAAACAAGCGGCACGTTGTATGCATTGTGATGTTCCTTTTT
GCCATCAAGGGATATTTTATGGGGGCAAACGGGCTGTCTCAGGTTGTCCAAATGATAATCATATTCCAGAGTGGAATGAT
TTGATTTATCGAGGACTACAAAGAAAAGCCTATGAACGTTTGATTTTAACCAATCCTTTTCCAGAGTTTACTGGCAGAGT
CTGTCCTGCTCCTTGTGAAAAATCTTGTGCCGAGGCCTTAAACGGTGCAGGAGTAACCATTAAAGATAATGAACGTTTCT
TGGGTGATTTAGGAAATGATGAAGGTTGGGTGGCTGAAATTGGCAAAGCTGCGGAGCCGACAGGTTATAAAATAGCGATT
ATTGGGTCTGGACCTGCCGGTCTGTCAGCTGCTTGGCGTCTTAATCAATTGGGACATAGCGTTACTGTTTTTGAAAAATC
AGATCGTTTGGGCGGCTTATTGATGTACGGCATTCCAAATATGAAATTGGATAAAAAAGTCGTTCAAAAACGAATTGATT
TAATGACAGAACTTGGTGTGACTTTTGTCACCAATACTGAGATTGGAAAGACAATCAGTTACGAAGACCTGTCAGTAAAT
TTTGATAGAATTATTTTGGCGATTGGCGCAGGAATTCCACGTGATTTAAATATTTCAGGCCGTGAAGCAGTTGGAATTCG
ATTTGCCATTGATTTTCTGACAGAAACGACTAAAACTGTCCTTGAAGAGGGCGAAGATAATATTAGTCAATCATTAAAAG
GAAAAAAAGTAGTAGTCATTGGTGGTGGAGATACAGGGAACGATTGTATCGGAACGGCAGTTCGTTTAGGAGCAGCTTCG
GTTTCTCAATTAGAAATCACACCAAGTTTACCCACTGATCGTTTAGATAACAACCCTTGGCCTGAGTGGCCAATGACCTT
GAGAGCAGGTTATGGGCAAAAAGAAGCAGAATTTGTGGGAAATACTGATTTGATAACCTATCAAATGACTGCAACAGCTT
TTCAAACAAATGCTGAAGGTCAACTTACGGGTTTAAAAGTAGCCAAAGTTGGACCTGACTTCAAGGCATTAGAAGGGACT
GAACAAGTAATTGAAGCGGATTTAGTCTTACTTGCTATGGGATTTGTTGGGACTGACACAGGATTACTGGATAAATTTGG
TATTCAAGAAATTTATGATGACTATCGAACAAATAATGACAAAGTTCTAGTTGCAGGCGATGCCAGACGAGGTCCATCTT
TGGTTATTTGGGCAATCCGTGAAGGAAGAAAAACAGCCGAAATTGTTGATGAACAATTAATAAAAGTCGCTACAGCATAA

Upstream 100 bases:

>100_bases
ATCATCATATTAAAAATGTTGAAGAAGATTTGGCTAAAACAGGCTTGTCAGGCGAAGCACTTACGATGACAACCTTTAAA
AAAGTAACGGAGGTGAGCTA

Downstream 100 bases:

>100_bases
TTTATCATTCTGTCAGTAAAAAAAAGTTTCTGTCAGTAAAAATGGCAGGAACTTTTTTGTGTCCAAGCTTTTGATGATAT
GCTATACTTATAAATAATCT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWND
LIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNDEGWVAEIGKAAEPTGYKIAI
IGSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNTEIGKTISYEDLSVN
FDRIILAIGAGIPRDLNISGREAVGIRFAIDFLTETTKTVLEEGEDNISQSLKGKKVVVIGGGDTGNDCIGTAVRLGAAS
VSQLEITPSLPTDRLDNNPWPEWPMTLRAGYGQKEAEFVGNTDLITYQMTATAFQTNAEGQLTGLKVAKVGPDFKALEGT
EQVIEADLVLLAMGFVGTDTGLLDKFGIQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEQLIKVATA

Sequences:

>Translated_479_residues
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWND
LIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNDEGWVAEIGKAAEPTGYKIAI
IGSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNTEIGKTISYEDLSVN
FDRIILAIGAGIPRDLNISGREAVGIRFAIDFLTETTKTVLEEGEDNISQSLKGKKVVVIGGGDTGNDCIGTAVRLGAAS
VSQLEITPSLPTDRLDNNPWPEWPMTLRAGYGQKEAEFVGNTDLITYQMTATAFQTNAEGQLTGLKVAKVGPDFKALEGT
EQVIEADLVLLAMGFVGTDTGLLDKFGIQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEQLIKVATA
>Mature_478_residues
ADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFYGGKRAVSGCPNDNHIPEWNDL
IYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEALNGAGVTIKDNERFLGDLGNDEGWVAEIGKAAEPTGYKIAII
GSGPAGLSAAWRLNQLGHSVTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNTEIGKTISYEDLSVNF
DRIILAIGAGIPRDLNISGREAVGIRFAIDFLTETTKTVLEEGEDNISQSLKGKKVVVIGGGDTGNDCIGTAVRLGAASV
SQLEITPSLPTDRLDNNPWPEWPMTLRAGYGQKEAEFVGNTDLITYQMTATAFQTNAEGQLTGLKVAKVGPDFKALEGTE
QVIEADLVLLAMGFVGTDTGLLDKFGIQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEQLIKVATA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=309, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1789606, Length=485, Percent_Identity=32.5773195876289, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788811, Length=485, Percent_Identity=31.1340206185567, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI87082180, Length=482, Percent_Identity=29.8755186721992, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=28.1531531531532, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1789243, Length=423, Percent_Identity=26.7139479905437, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=492, Percent_Identity=43.0894308943089, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17543860, Length=185, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=43.0612244897959, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=43.2098765432099, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=43.2098765432099, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=43.2098765432099, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=43.2098765432099, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640763, Length=316, Percent_Identity=29.1139240506329, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18858217, Length=316, Percent_Identity=29.1139240506329, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52365; Mature: 52234

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHEECCCCHHHCCCEE
GGKRAVSGCPNDNHIPEWNDLIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEAL
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCHHHHHHHHH
NGAGVTIKDNERFLGDLGNDEGWVAEIGKAAEPTGYKIAIIGSGPAGLSAAWRLNQLGHS
CCCCEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCE
VTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNTEIGKTISYEDLSVN
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCC
FDRIILAIGAGIPRDLNISGREAVGIRFAIDFLTETTKTVLEEGEDNISQSLKGKKVVVI
HHHEEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEE
GGGDTGNDCIGTAVRLGAASVSQLEITPSLPTDRLDNNPWPEWPMTLRAGYGQKEAEFVG
ECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCC
NTDLITYQMTATAFQTNAEGQLTGLKVAKVGPDFKALEGTEQVIEADLVLLAMGFVGTDT
CCEEEEEEEEEEEEECCCCCCEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCC
GLLDKFGIQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEQLIKVATA
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADPNGFLNYPRNDNPYRELAERIKDFAELQVPLSTEERQKQAARCMHCDVPFCHQGIFY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHEECCCCHHHCCCEE
GGKRAVSGCPNDNHIPEWNDLIYRGLQRKAYERLILTNPFPEFTGRVCPAPCEKSCAEAL
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCHHHHHHHHH
NGAGVTIKDNERFLGDLGNDEGWVAEIGKAAEPTGYKIAIIGSGPAGLSAAWRLNQLGHS
CCCCEEEECCCHHCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCE
VTVFEKSDRLGGLLMYGIPNMKLDKKVVQKRIDLMTELGVTFVTNTEIGKTISYEDLSVN
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCC
FDRIILAIGAGIPRDLNISGREAVGIRFAIDFLTETTKTVLEEGEDNISQSLKGKKVVVI
HHHEEEEECCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEE
GGGDTGNDCIGTAVRLGAASVSQLEITPSLPTDRLDNNPWPEWPMTLRAGYGQKEAEFVG
ECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCC
NTDLITYQMTATAFQTNAEGQLTGLKVAKVGPDFKALEGTEQVIEADLVLLAMGFVGTDT
CCEEEEEEEEEEEEECCCCCCEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCC
GLLDKFGIQEIYDDYRTNNDKVLVAGDARRGPSLVIWAIREGRKTAEIVDEQLIKVATA
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]