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The map label for this gene is glpF2

Identifier: 15673226

GI number: 15673226

Start: 1269307

End: 1270044

Strand: Reverse

Name: glpF2

Synonym: L0015

Alternate gene names: 15673226

Gene position: 1270044-1269307 (Counterclockwise)

Preceding gene: 15673227

Following gene: 15673224

Centisome position: 53.69

GC content: 43.9

Gene sequence:

>738_bases
ATGCACTCAGAAATGGTACAGCTACTTGGTGAATTTTTAGGAACATTTATCTTAATACTTCTTGGTAACGGGGTCGTTTC
AGGCGTTGTCCTGAACAAAACGAAAGCAACAGGAGCGGGTTGGGTTGCTATCACTCTCGGTTGGGGATTTGCAGTAATGA
TGGGGGTTTACATTTCTGGATTTATGTCGCCAGCCCATTTGAATCCAGCAGTAACAATTGCAATGGCAATGATTGGGAGC
TTTAGCTGGAGTTTGGTTCTTCCTTATATTATTGCGCAAATGCTTGGTGCAATGGTAGCCAGCATTATCCTCTACCTAAT
GTTTTACCCTCACTATGCTGAAACAAAGAATCCAGCAGATATCTTGGGCACGTTTTCTACAGGTCCTGCCATTCGCCAGA
CGAGTTCAAACTTAATCAGTGAGATTGTGGGAACAGCAGTCTTAACGACAGGTATTCTGGCCTTTGGTCAATACGCAATT
ACGCAAACTTCAGGTGTTTCACCTCTTTTAGTGGGTGCTATCATCACAGCTATTGGTTTATCACTTGGTGCAACAACAGG
ATACTCTTTAAATCCCGCACGTGACTTAGGTCCCCGTATCATGCACGCTATCTTGCCTATCAAAGGCAAAGGGGATTCAG
ACTGGTCTTACGCATGGATTCCTGTCGTAGGTCCAATCATTGGAGGTAGCTTGGGCGCCCTTCTCTTTAACATGGTTATT
CAATTTGCAAGTAAATAA

Upstream 100 bases:

>100_bases
CTTTGGAAGAAGCACTTCGTGAATCGGACTTGTCGGATTTGAAGAAGTAAAAAATGCAAAAATGATTGAAACAAATAAAA
AATAGAAATAAGGGGTCAAT

Downstream 100 bases:

>100_bases
ACTGAAATAATATATAAAAAAAGAGTTAGACAAAGTTCTAACTCTTTTTATTAAAAATAAATGAGCCATAAGAAAAAATT
TTCTATAATCAAAGTCACAA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MHSEMVQLLGEFLGTFILILLGNGVVSGVVLNKTKATGAGWVAITLGWGFAVMMGVYISGFMSPAHLNPAVTIAMAMIGS
FSWSLVLPYIIAQMLGAMVASIILYLMFYPHYAETKNPADILGTFSTGPAIRQTSSNLISEIVGTAVLTTGILAFGQYAI
TQTSGVSPLLVGAIITAIGLSLGATTGYSLNPARDLGPRIMHAILPIKGKGDSDWSYAWIPVVGPIIGGSLGALLFNMVI
QFASK

Sequences:

>Translated_245_residues
MHSEMVQLLGEFLGTFILILLGNGVVSGVVLNKTKATGAGWVAITLGWGFAVMMGVYISGFMSPAHLNPAVTIAMAMIGS
FSWSLVLPYIIAQMLGAMVASIILYLMFYPHYAETKNPADILGTFSTGPAIRQTSSNLISEIVGTAVLTTGILAFGQYAI
TQTSGVSPLLVGAIITAIGLSLGATTGYSLNPARDLGPRIMHAILPIKGKGDSDWSYAWIPVVGPIIGGSLGALLFNMVI
QFASK
>Mature_245_residues
MHSEMVQLLGEFLGTFILILLGNGVVSGVVLNKTKATGAGWVAITLGWGFAVMMGVYISGFMSPAHLNPAVTIAMAMIGS
FSWSLVLPYIIAQMLGAMVASIILYLMFYPHYAETKNPADILGTFSTGPAIRQTSSNLISEIVGTAVLTTGILAFGQYAI
TQTSGVSPLLVGAIITAIGLSLGATTGYSLNPARDLGPRIMHAILPIKGKGDSDWSYAWIPVVGPIIGGSLGALLFNMVI
QFASK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=34.9397590361446, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=34.2741935483871, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI22538420, Length=247, Percent_Identity=32.7935222672065, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI4502187, Length=254, Percent_Identity=33.4645669291339, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI310133356, Length=219, Percent_Identity=29.2237442922374, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI310114181, Length=219, Percent_Identity=29.2237442922374, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI37694062, Length=236, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502179, Length=236, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4755125, Length=239, Percent_Identity=29.2887029288703, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4502181, Length=239, Percent_Identity=29.2887029288703, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI86792455, Length=234, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=34.1269841269841, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787101, Length=192, Percent_Identity=31.7708333333333, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=237, Percent_Identity=32.4894514767932, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71992966, Length=246, Percent_Identity=29.6747967479675, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=257, Percent_Identity=29.9610894941634, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=257, Percent_Identity=29.9610894941634, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=254, Percent_Identity=31.1023622047244, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=32.2448979591837, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=32.2448979591837, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=238, Percent_Identity=29.8319327731092, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=239, Percent_Identity=27.1966527196653, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321054, Length=219, Percent_Identity=32.8767123287671, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=27.112676056338, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=29.3617021276596, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=29.3617021276596, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652751, Length=234, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922038, Length=234, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24762342, Length=249, Percent_Identity=25.3012048192771, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25641; Mature: 25641

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSEMVQLLGEFLGTFILILLGNGVVSGVVLNKTKATGAGWVAITLGWGFAVMMGVYISG
CCHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHH
FMSPAHLNPAVTIAMAMIGSFSWSLVLPYIIAQMLGAMVASIILYLMFYPHYAETKNPAD
HCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ILGTFSTGPAIRQTSSNLISEIVGTAVLTTGILAFGQYAITQTSGVSPLLVGAIITAIGL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SLGATTGYSLNPARDLGPRIMHAILPIKGKGDSDWSYAWIPVVGPIIGGSLGALLFNMVI
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHH
QFASK
HHHCC
>Mature Secondary Structure
MHSEMVQLLGEFLGTFILILLGNGVVSGVVLNKTKATGAGWVAITLGWGFAVMMGVYISG
CCHHHHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHH
FMSPAHLNPAVTIAMAMIGSFSWSLVLPYIIAQMLGAMVASIILYLMFYPHYAETKNPAD
HCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ILGTFSTGPAIRQTSSNLISEIVGTAVLTTGILAFGQYAITQTSGVSPLLVGAIITAIGL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SLGATTGYSLNPARDLGPRIMHAILPIKGKGDSDWSYAWIPVVGPIIGGSLGALLFNMVI
HHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHH
QFASK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]