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The map label for this gene is recJ

Identifier: 15672614

GI number: 15672614

Start: 620478

End: 622706

Strand: Direct

Name: recJ

Synonym: L0259

Alternate gene names: 15672614

Gene position: 620478-622706 (Clockwise)

Preceding gene: 15672613

Following gene: 15672615

Centisome position: 26.23

GC content: 36.97

Gene sequence:

>2229_bases
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAACATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCATCATCAGTCATGAAATCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCCGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCCCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGCTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGATGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGGATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCGATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCTAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGATACCTGGAGATTTAGCAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
GGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Upstream 100 bases:

>100_bases
ATTAAATAAAAGAAAAATAATCTCTAAACTTAGCCATTTTTTTCGAGTAATTTATGCTAAAATAATTGAATTGAGCATAG
AAAAGATAAAGAGAATTAAA

Downstream 100 bases:

>100_bases
TTTCGTTTTTATAAAAAGATAGTGACGAAATCAGCTATACTCATAATAATAAAGCTGTCAGTAAAAATAACGGACTTATG
TTAGAAAAAAGGAGCACTGA

Product: single-stranded DNA specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEEIPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Sequences:

>Translated_742_residues
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEEIPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK
>Mature_742_residues
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEEIPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=577, Percent_Identity=33.4488734835355, Blast_Score=259, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 83540; Mature: 83540

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PFLLHDMEKATGRILSAIEQGENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRF
CHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
TDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQENGVDVIVTDHHSIPDQLPP
CCCCCCCCCEEEEEEECCCEEEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCC
AYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
CEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIEL
EEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHHCCCCCCCCCCEEE
LTGWDEDEAHEIAKMIDQKNSERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVA
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
GRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAHRELFIAFGGHKQAAGMTLSL
HHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEH
ENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSN
EEHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECC
SWNGNTSLQLMIEDADSVGVELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEEIPG
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGTREEFARLYKAIYQFPEFDIR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHH
YKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
HHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHH
VKMQELFALAPVKEIYQNLLEK
HHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PFLLHDMEKATGRILSAIEQGENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRF
CHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
TDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQENGVDVIVTDHHSIPDQLPP
CCCCCCCCCEEEEEEECCCEEEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCC
AYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
CEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIEL
EEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHHCCCCCCCCCCEEE
LTGWDEDEAHEIAKMIDQKNSERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVA
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
GRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAHRELFIAFGGHKQAAGMTLSL
HHHHHHHCCCEEEEECCCCCEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEH
ENVEAVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCC
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSN
EEHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECC
SWNGNTSLQLMIEDADSVGVELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEEIPG
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
DLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGTREEFARLYKAIYQFPEFDIR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHH
YKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
HHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHH
VKMQELFALAPVKEIYQNLLEK
HHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]