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The map label for this gene is optB

Identifier: 15672327

GI number: 15672327

Start: 347018

End: 347929

Strand: Direct

Name: optB

Synonym: L147009

Alternate gene names: 15672327

Gene position: 347018-347929 (Clockwise)

Preceding gene: 15672326

Following gene: 15672328

Centisome position: 14.67

GC content: 37.72

Gene sequence:

>912_bases
ATGATTAAATATATTCTTAAACGTTTGGGGCTTTTGCTTCTGACTTTGTTCCTGATTGTGACATTGACTTTCTTTATGAT
GCAAGTCATGCCCGGAACACCTTTCTCAAATCCCAAACTAACGCCAGATCAACTGGAAATTTTAAAACATGCCTATGGTT
TAGATAAACCGCTTTGGCAACAATACTTTATCTATGTAGGACACATGTTCACAGGTAATTTTGGAACATCATTTGTTTAT
ACAAACCAACCTGTTATTACAATGATCGCTCAACGTTTACCAGTTTCAATGCAACTTGGAGTTCAAGCGCTTATTCTTGG
GACAATTCTTGGAGCCTTTATGGGAAAAGCTTCCGCTCGTCGTAAAAATGGCATCCTTGATGGAATTTTCGGTTTTGTTT
CAGTCCTTGGGATTTCAGTTCCTTCATTCGTAATTGGTACCTTAATCCTTCTTTACTTAGGATTTAATTTGAATCTTTTC
CCAATTTCTGGTTGGGGAACCTTCTCACAAACAATTATGCCAACAATCGCTCTTTCTTTTGCTCCAATGGCTGTGGTAAC
TCGTTTTGTTCGTTCTGAAATGATTGAGTCTTTATCTTCTGACTACATCTTGTTAGCTCGTGCTAAAGGACTTTCTGAAA
AAGAAGTGGTCAATAAACATGCATTGCGTAATTCATTGATTCCAATGTTGACTTTGATTGGGCCAATGGCTGCCAATCTT
TTGACAGGTTCGGTCTTGATTGAAAAAATCTTTTCAATCCCCGGGATTGGTTCACAATTTGTTGATTCAATTCCAGCCAA
AGACTTCCCAGTTATTATGGCAACAACAATTGTCTATGCAGTAATCTTGATGCTATTTATCTTAGTTACAGATATTCTGA
CTGCAATTGTTGATCCACGAGTTCGTCTCTAG

Upstream 100 bases:

>100_bases
TCAAAACAGCTTACCTCAAATAATAATAGAGTTTTAGAGCAAGAAAGTTAGGATTGGCAGATTGCCAACCCTATCTTTTG
CTTAGATTAGGAAATTTTTT

Downstream 100 bases:

>100_bases
AAAGGGTTTGTCGAAATGGAAAATTTAAATAAAGACTTCACCCTTGTTGGTTCAAAGGGTTCAGATTCAACAGAAAAAAT
TGCGAAACCTGCCTTGAGTT

Product: oligopeptide ABC trasporter permease protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KB [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIKYILKRLGLLLLTLFLIVTLTFFMMQVMPGTPFSNPKLTPDQLEILKHAYGLDKPLWQQYFIYVGHMFTGNFGTSFVY
TNQPVITMIAQRLPVSMQLGVQALILGTILGAFMGKASARRKNGILDGIFGFVSVLGISVPSFVIGTLILLYLGFNLNLF
PISGWGTFSQTIMPTIALSFAPMAVVTRFVRSEMIESLSSDYILLARAKGLSEKEVVNKHALRNSLIPMLTLIGPMAANL
LTGSVLIEKIFSIPGIGSQFVDSIPAKDFPVIMATTIVYAVILMLFILVTDILTAIVDPRVRL

Sequences:

>Translated_303_residues
MIKYILKRLGLLLLTLFLIVTLTFFMMQVMPGTPFSNPKLTPDQLEILKHAYGLDKPLWQQYFIYVGHMFTGNFGTSFVY
TNQPVITMIAQRLPVSMQLGVQALILGTILGAFMGKASARRKNGILDGIFGFVSVLGISVPSFVIGTLILLYLGFNLNLF
PISGWGTFSQTIMPTIALSFAPMAVVTRFVRSEMIESLSSDYILLARAKGLSEKEVVNKHALRNSLIPMLTLIGPMAANL
LTGSVLIEKIFSIPGIGSQFVDSIPAKDFPVIMATTIVYAVILMLFILVTDILTAIVDPRVRL
>Mature_303_residues
MIKYILKRLGLLLLTLFLIVTLTFFMMQVMPGTPFSNPKLTPDQLEILKHAYGLDKPLWQQYFIYVGHMFTGNFGTSFVY
TNQPVITMIAQRLPVSMQLGVQALILGTILGAFMGKASARRKNGILDGIFGFVSVLGISVPSFVIGTLILLYLGFNLNLF
PISGWGTFSQTIMPTIALSFAPMAVVTRFVRSEMIESLSSDYILLARAKGLSEKEVVNKHALRNSLIPMLTLIGPMAANL
LTGSVLIEKIFSIPGIGSQFVDSIPAKDFPVIMATTIVYAVILMLFILVTDILTAIVDPRVRL

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=36.3934426229508, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787053, Length=305, Percent_Identity=35.4098360655738, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=33.2344213649852, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1789888, Length=308, Percent_Identity=30.5194805194805, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787761, Length=328, Percent_Identity=32.3170731707317, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788504, Length=363, Percent_Identity=26.4462809917355, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787550, Length=281, Percent_Identity=24.9110320284698, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33357; Mature: 33357

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYILKRLGLLLLTLFLIVTLTFFMMQVMPGTPFSNPKLTPDQLEILKHAYGLDKPLWQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHH
QYFIYVGHMFTGNFGTSFVYTNQPVITMIAQRLPVSMQLGVQALILGTILGAFMGKASAR
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKNGILDGIFGFVSVLGISVPSFVIGTLILLYLGFNLNLFPISGWGTFSQTIMPTIALSF
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
APMAVVTRFVRSEMIESLSSDYILLARAKGLSEKEVVNKHALRNSLIPMLTLIGPMAANL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGSVLIEKIFSIPGIGSQFVDSIPAKDFPVIMATTIVYAVILMLFILVTDILTAIVDPR
HHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRL
CCC
>Mature Secondary Structure
MIKYILKRLGLLLLTLFLIVTLTFFMMQVMPGTPFSNPKLTPDQLEILKHAYGLDKPLWQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHH
QYFIYVGHMFTGNFGTSFVYTNQPVITMIAQRLPVSMQLGVQALILGTILGAFMGKASAR
HHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKNGILDGIFGFVSVLGISVPSFVIGTLILLYLGFNLNLFPISGWGTFSQTIMPTIALSF
HHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
APMAVVTRFVRSEMIESLSSDYILLARAKGLSEKEVVNKHALRNSLIPMLTLIGPMAANL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGSVLIEKIFSIPGIGSQFVDSIPAKDFPVIMATTIVYAVILMLFILVTDILTAIVDPR
HHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]