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The map label for this gene is optA

Identifier: 15672326

GI number: 15672326

Start: 345303

End: 346940

Strand: Direct

Name: optA

Synonym: L145267

Alternate gene names: 15672326

Gene position: 345303-346940 (Clockwise)

Preceding gene: 15672325

Following gene: 15672327

Centisome position: 14.6

GC content: 38.52

Gene sequence:

>1638_bases
ATGAAGACTTGGAAAAAAGTTACCTTAGGTACAGTTGCACTTGGTTCGGCAGCTTTGCTTGCAGCATGCGGCAATAGCGG
TTCTAGCTCATCGTCATCATCAAAAGACATTCAATGGAATTTGACATCACCTATTCAAACATTGGATTCATCACTCGCGA
CAGATACATATTCAAATATCATTATTGGTAATACCAATGCTGGTTTGACACGTGTGGACAAAGATGGTAAAGCACAGCCA
GAATTGGCAAAATCAGTTGATGTATCAAAAGATGGCTTGACATATACTTTCCATCTTCGTGATGGCTTGAAATGGTCAAA
CGGTGATGCTTTGACAGCTAAAGACTTCGTTTATTCTTGGCAACGTGCGGTTGATCCAGCGACTGCTTCAGAATACGGAT
ACCTCCTTGGCCCAGTTAAAAACGCCAACGAGATTAATGGTGGTAAAGCTGATAAATCAACACTTGGTGTTAAAGCAACT
GATGACAAAACATTTGTTGTTACCCTTGCACAACCAACACCATACTTTGAATTCTTGACAGCTAACTCTGTTTATTACCC
ACTCAACCAAAAAGTGGTTGAAAAATATGGGAAACAATACGGAACATCTTCAGAGAAAATGGTTTACTCTGGACCATATA
AATTTGAAAAATCAAAAGGTTGGAACGGTTCAAACCAAACATTCTCAATCGTGAAGAATGACGATTATTGGGATAAGAGC
GCAGTTAAAACTAAAGAAATCGACTTCCAAGTGGTTCAAGACCCTAAAACTTCATTTAACCTTTATAAACAAGGTAAGAT
TGTCCAAGCAAGTATTGGTGACCCAGACCTTTTCAAAGCAAATAAAAATAATAAAGATGTCGTATCTTTGGATGAAGCAA
CAACAGCTTACATTCAATATAATCAATCTGGGAAAAATAAAGCTCTTGCTAACAAGAACATTCGTGAAGCCTTCAACCTT
GCTACTGACCGCCAACAATATGTTGATACAGTAACGCCTGCTTCGAATCCTGCGACAGGCTTAACACCAGCGGGTATGGC
TAAAACAAATACTGGTGTAGACTTTGCTAAGTATGCAGCACAACCATATAAATACGATGCCACAGCAGCTAAAGCAGCTT
GGGCTAAAGGATTGAGCGAAATTGGCGAAACTAAACTTACTTTGACTTTAACAACTGATGATACATCAGCTTCTAAAGAC
TCAGCAACTTTCCTTAAACAAGCATGGGAAAAACAACTTCCAGGTTTGACTTTGAATATCAAATCAGTACCATTTAAACA
ACGTTTGAATGACTCTACTACTGGTAACTTTGATATGGTTATCTCACTTTGGGGTGGTGACTATGCTGAACCGTCAACTT
TCCTTGATTTATTTGTAAAAGATGGACCAAATAATAATGGTAAAATCAATAACGTTACTTATGATAAAGCCATTAAAGCA
GCTGAAGTAACAGATGCGCTTGATCCTGAAAAGCACTATGCAGACTACAAAGCGGCTGAAGAATCACTTTATACAGAATC
AAATCTCAATCCGCTGTACTTCCGTACAACTCCAGTCCTCCGTAATCCTAACCTTAAGGATGTACGCTTTGGTTCAACTG
GTTTAATGTATGACTTCAAAACAGCTTACCTCAAATAA

Upstream 100 bases:

>100_bases
TACAATTCAGGTTGAATTAAGTACAATACCAATATAATAAATTTTCTGACAATAATAAAAATTGCGAAGTCAGACACAAT
TCATGAAAGAGGGAAATTAC

Downstream 100 bases:

>100_bases
TAATAGAGTTTTAGAGCAAGAAAGTTAGGATTGGCAGATTGCCAACCCTATCTTTTGCTTAGATTAGGAAATTTTTTATG
ATTAAATATATTCTTAAACG

Product: oligopeptide ABC transporter substrate binding protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKTWKKVTLGTVALGSAALLAACGNSGSSSSSSSKDIQWNLTSPIQTLDSSLATDTYSNIIIGNTNAGLTRVDKDGKAQP
ELAKSVDVSKDGLTYTFHLRDGLKWSNGDALTAKDFVYSWQRAVDPATASEYGYLLGPVKNANEINGGKADKSTLGVKAT
DDKTFVVTLAQPTPYFEFLTANSVYYPLNQKVVEKYGKQYGTSSEKMVYSGPYKFEKSKGWNGSNQTFSIVKNDDYWDKS
AVKTKEIDFQVVQDPKTSFNLYKQGKIVQASIGDPDLFKANKNNKDVVSLDEATTAYIQYNQSGKNKALANKNIREAFNL
ATDRQQYVDTVTPASNPATGLTPAGMAKTNTGVDFAKYAAQPYKYDATAAKAAWAKGLSEIGETKLTLTLTTDDTSASKD
SATFLKQAWEKQLPGLTLNIKSVPFKQRLNDSTTGNFDMVISLWGGDYAEPSTFLDLFVKDGPNNNGKINNVTYDKAIKA
AEVTDALDPEKHYADYKAAEESLYTESNLNPLYFRTTPVLRNPNLKDVRFGSTGLMYDFKTAYLK

Sequences:

>Translated_545_residues
MKTWKKVTLGTVALGSAALLAACGNSGSSSSSSSKDIQWNLTSPIQTLDSSLATDTYSNIIIGNTNAGLTRVDKDGKAQP
ELAKSVDVSKDGLTYTFHLRDGLKWSNGDALTAKDFVYSWQRAVDPATASEYGYLLGPVKNANEINGGKADKSTLGVKAT
DDKTFVVTLAQPTPYFEFLTANSVYYPLNQKVVEKYGKQYGTSSEKMVYSGPYKFEKSKGWNGSNQTFSIVKNDDYWDKS
AVKTKEIDFQVVQDPKTSFNLYKQGKIVQASIGDPDLFKANKNNKDVVSLDEATTAYIQYNQSGKNKALANKNIREAFNL
ATDRQQYVDTVTPASNPATGLTPAGMAKTNTGVDFAKYAAQPYKYDATAAKAAWAKGLSEIGETKLTLTLTTDDTSASKD
SATFLKQAWEKQLPGLTLNIKSVPFKQRLNDSTTGNFDMVISLWGGDYAEPSTFLDLFVKDGPNNNGKINNVTYDKAIKA
AEVTDALDPEKHYADYKAAEESLYTESNLNPLYFRTTPVLRNPNLKDVRFGSTGLMYDFKTAYLK
>Mature_545_residues
MKTWKKVTLGTVALGSAALLAACGNSGSSSSSSSKDIQWNLTSPIQTLDSSLATDTYSNIIIGNTNAGLTRVDKDGKAQP
ELAKSVDVSKDGLTYTFHLRDGLKWSNGDALTAKDFVYSWQRAVDPATASEYGYLLGPVKNANEINGGKADKSTLGVKAT
DDKTFVVTLAQPTPYFEFLTANSVYYPLNQKVVEKYGKQYGTSSEKMVYSGPYKFEKSKGWNGSNQTFSIVKNDDYWDKS
AVKTKEIDFQVVQDPKTSFNLYKQGKIVQASIGDPDLFKANKNNKDVVSLDEATTAYIQYNQSGKNKALANKNIREAFNL
ATDRQQYVDTVTPASNPATGLTPAGMAKTNTGVDFAKYAAQPYKYDATAAKAAWAKGLSEIGETKLTLTLTTDDTSASKD
SATFLKQAWEKQLPGLTLNIKSVPFKQRLNDSTTGNFDMVISLWGGDYAEPSTFLDLFVKDGPNNNGKINNVTYDKAIKA
AEVTDALDPEKHYADYKAAEESLYTESNLNPLYFRTTPVLRNPNLKDVRFGSTGLMYDFKTAYLK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=29.8651252408478, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787495, Length=491, Percent_Identity=30.5498981670061, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=28.6778398510242, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787052, Length=368, Percent_Identity=28.804347826087, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789887, Length=501, Percent_Identity=25.3493013972056, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=504, Percent_Identity=22.6190476190476, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59698; Mature: 59698

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWKKVTLGTVALGSAALLAACGNSGSSSSSSSKDIQWNLTSPIQTLDSSLATDTYSNI
CCCCCEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCE
IIGNTNAGLTRVDKDGKAQPELAKSVDVSKDGLTYTFHLRDGLKWSNGDALTAKDFVYSW
EEECCCCCCEEECCCCCCCCHHHHHCCCCCCCEEEEEEECCCCEECCCCEEEHHHHHHHH
QRAVDPATASEYGYLLGPVKNANEINGGKADKSTLGVKATDDKTFVVTLAQPTPYFEFLT
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEEEE
ANSVYYPLNQKVVEKYGKQYGTSSEKMVYSGPYKFEKSKGWNGSNQTFSIVKNDDYWDKS
CCCEEECCCHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCC
AVKTKEIDFQVVQDPKTSFNLYKQGKIVQASIGDPDLFKANKNNKDVVSLDEATTAYIQY
CCEEECCCEEEECCCCCCCCCEECCCEEEEECCCCCCEECCCCCCCEEEECCCEEEEEEE
NQSGKNKALANKNIREAFNLATDRQQYVDTVTPASNPATGLTPAGMAKTNTGVDFAKYAA
CCCCCCCHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
QPYKYDATAAKAAWAKGLSEIGETKLTLTLTTDDTSASKDSATFLKQAWEKQLPGLTLNI
CCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
KSVPFKQRLNDSTTGNFDMVISLWGGDYAEPSTFLDLFVKDGPNNNGKINNVTYDKAIKA
ECCCHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCEECEEEHHHHHHH
AEVTDALDPEKHYADYKAAEESLYTESNLNPLYFRTTPVLRNPNLKDVRFGSTGLMYDFK
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCEEEECCCCEEEECH
TAYLK
HHHCC
>Mature Secondary Structure
MKTWKKVTLGTVALGSAALLAACGNSGSSSSSSSKDIQWNLTSPIQTLDSSLATDTYSNI
CCCCCEEEEEEEHHCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCE
IIGNTNAGLTRVDKDGKAQPELAKSVDVSKDGLTYTFHLRDGLKWSNGDALTAKDFVYSW
EEECCCCCCEEECCCCCCCCHHHHHCCCCCCCEEEEEEECCCCEECCCCEEEHHHHHHHH
QRAVDPATASEYGYLLGPVKNANEINGGKADKSTLGVKATDDKTFVVTLAQPTPYFEFLT
HHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCEEEEE
ANSVYYPLNQKVVEKYGKQYGTSSEKMVYSGPYKFEKSKGWNGSNQTFSIVKNDDYWDKS
CCCEEECCCHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCC
AVKTKEIDFQVVQDPKTSFNLYKQGKIVQASIGDPDLFKANKNNKDVVSLDEATTAYIQY
CCEEECCCEEEECCCCCCCCCEECCCEEEEECCCCCCEECCCCCCCEEEECCCEEEEEEE
NQSGKNKALANKNIREAFNLATDRQQYVDTVTPASNPATGLTPAGMAKTNTGVDFAKYAA
CCCCCCCHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
QPYKYDATAAKAAWAKGLSEIGETKLTLTLTTDDTSASKDSATFLKQAWEKQLPGLTLNI
CCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
KSVPFKQRLNDSTTGNFDMVISLWGGDYAEPSTFLDLFVKDGPNNNGKINNVTYDKAIKA
ECCCHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCEECEEEHHHHHHH
AEVTDALDPEKHYADYKAAEESLYTESNLNPLYFRTTPVLRNPNLKDVRFGSTGLMYDFK
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCEEEECCCCEEEECH
TAYLK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]