Click here to switch to the map view.

The map label for this gene is cysD

Identifier: 15672055

GI number: 15672055

Start: 75972

End: 77252

Strand: Reverse

Name: cysD

Synonym: L75975

Alternate gene names: 15672055

Gene position: 77252-75972 (Counterclockwise)

Preceding gene: 15672056

Following gene: 15672054

Centisome position: 3.27

GC content: 39.89

Gene sequence:

>1281_bases
ATGACTAATCACAATTATAAATTCGACACTTTGCAAGTCCATGCAGGACAAGTCCCTGATCCTGTCACGGGTTCACGCGC
CGTTCCGCTCTATCAAACAACTTCTTTCGTTTTTAACAATTCAGACCATGCCGAAGCTCGTTTTGCTTTACAAGATCCTG
GAGCTATTTATTCACGTTTAGGAAATCCAACCAACGATGTTTTTGAAGCACGCATCGCAGCTCTTGAAGGTGGAAGTGCA
GCCCTTGGTGTTGGTTCTGGCTCAGCCGCTATTACCTATGCCATCTTGAATATCGCTACAGTCGGTGATAATATTGTTTC
CGCAAGTACCCTTTATGGTGGAACCTATCACCTTTTTTCTGGGACTTTACCAAAATATGGAATTACAACTAAATTTGTCA
ATCCAGATGACCCGAAGAATTTTGAAGAGGCGATTGATGAAAAAACCAAAGCTATTTATTATGAAACTTTGGGCAATCCG
GGAAATAATGTGATTGATTATGATGCCATTGGTCAAATTGCTAAAAAACATGGAATTCCCGTTATTGTTGATGCAACGTT
TACTACCCCTGTGACCTTTAAACCATTTGAACATGGTGCTAATGTAATTGTTCATTCAGCAACGAAATTCATTGGCGGTC
ATGGTACTTCTATTGGTGGAGTCATCGTTGATGGCGGAAACTTTGATTGGGCAAATGGTAATTTTCCTGATTTTACACAA
GCTGATGAAAGCTACAATGGGATTAAATTTGCCGAATTGGGTGAAATTGCTTTTGTGACTCGGGTTAGAGCTATTTTATT
ACGTGATACGGGTGCGGCTTTATCACCTTTTCATTCTTGGCTTTTCTTACAGGGGCTAGAAACACTCTCACTCCGGGTAG
AACGTCACATCTCCAATACTAAAAAGATTGTAGAATTTTTAGACAATCATCCTAAGGTGGAACTTGTTAACCATCCTCTG
CTTGAAAGTAATTCCTATCATGCGCTCTATCAGAAATATTATCCAAAAGATGCTGGATCTATCTTTACCTTTGAACTCAA
AGACAAAGATGAGAAAAAAGCGCGTGATTTGATTGATCATCTTGAAATTTTCTCACTTCTAGCCAACGTTGGAGATACCA
AATCATTGGCCATTCATCCTGCTTCGACCACTCACCAGCAGCTGAATGCCGAAGAACTTGCTAGTGCAGGGATTTCCAAA
GGAACCATTCGATTATCGGTTGGTATTGAAGATGTAACTGACTTGATTGCTGATTTAGAGCAAGCATTAGAAAAAATATA
A

Upstream 100 bases:

>100_bases
AAAGCTATGGTTCAAGAATTGCTGACGAATAATCAACGAATGTGATTAACGACTATTTTTTATACTAACTAAAATTTTCA
AAAATAGGAGAAAAAAAACT

Downstream 100 bases:

>100_bases
AAAAATGAGCGGTAACCAGCCGCTCATTCTTTTGTTTAGGAGTAAACTTTATGAAAAATTTTATTGGAATAAATATATTA
TAGTCAACTTGACTTAAGTT

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTNHNYKFDTLQVHAGQVPDPVTGSRAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSA
ALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNP
GNNVIDYDAIGQIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGNFPDFTQ
ADESYNGIKFAELGEIAFVTRVRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHISNTKKIVEFLDNHPKVELVNHPL
LESNSYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNAEELASAGISK
GTIRLSVGIEDVTDLIADLEQALEKI

Sequences:

>Translated_426_residues
MTNHNYKFDTLQVHAGQVPDPVTGSRAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSA
ALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNP
GNNVIDYDAIGQIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGNFPDFTQ
ADESYNGIKFAELGEIAFVTRVRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHISNTKKIVEFLDNHPKVELVNHPL
LESNSYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNAEELASAGISK
GTIRLSVGIEDVTDLIADLEQALEKI
>Mature_425_residues
TNHNYKFDTLQVHAGQVPDPVTGSRAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRLGNPTNDVFEARIAALEGGSAA
LGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFSGTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPG
NNVIDYDAIGQIAKKHGIPVIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGNFPDFTQA
DESYNGIKFAELGEIAFVTRVRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHISNTKKIVEFLDNHPKVELVNHPLL
ESNSYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDHLEIFSLLANVGDTKSLAIHPASTTHQQLNAEELASAGISKG
TIRLSVGIEDVTDLIADLEQALEKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=33.2547169811321, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=30.188679245283, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=29.5508274231678, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1790375, Length=414, Percent_Identity=31.6425120772947, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=26.046511627907, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=32.9297820823245, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=33.5714285714286, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=33.8095238095238, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=49.5391705069124, Blast_Score=355, Evalue=9e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=425, Percent_Identity=31.7647058823529, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=333, Percent_Identity=27.3273273273273, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=25.3968253968254, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=410, Percent_Identity=23.6585365853659, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=33.4123222748815, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46269; Mature: 46138

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHNYKFDTLQVHAGQVPDPVTGSRAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRL
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHC
GNPTNDVFEARIAALEGGSAALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFS
CCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEEC
GTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPGNNVIDYDAIGQIAKKHGIP
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEECHHHHHHHHHHCCCC
VIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGNFPDFTQ
EEEEECCCCCEEECCHHCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ADESYNGIKFAELGEIAFVTRVRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHISNT
CCCCCCCEEEHHHCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIVEFLDNHPKVELVNHPLLESNSYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDH
HHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LEIFSLLANVGDTKSLAIHPASTTHQQLNAEELASAGISKGTIRLSVGIEDVTDLIADLE
HHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEKI
HHHHCC
>Mature Secondary Structure 
TNHNYKFDTLQVHAGQVPDPVTGSRAVPLYQTTSFVFNNSDHAEARFALQDPGAIYSRL
CCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHC
GNPTNDVFEARIAALEGGSAALGVGSGSAAITYAILNIATVGDNIVSASTLYGGTYHLFS
CCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEEC
GTLPKYGITTKFVNPDDPKNFEEAIDEKTKAIYYETLGNPGNNVIDYDAIGQIAKKHGIP
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEEEEECCCCCCCEECHHHHHHHHHHCCCC
VIVDATFTTPVTFKPFEHGANVIVHSATKFIGGHGTSIGGVIVDGGNFDWANGNFPDFTQ
EEEEECCCCCEEECCHHCCCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ADESYNGIKFAELGEIAFVTRVRAILLRDTGAALSPFHSWLFLQGLETLSLRVERHISNT
CCCCCCCEEEHHHCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIVEFLDNHPKVELVNHPLLESNSYHALYQKYYPKDAGSIFTFELKDKDEKKARDLIDH
HHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
LEIFSLLANVGDTKSLAIHPASTTHQQLNAEELASAGISKGTIRLSVGIEDVTDLIADLE
HHHHHHHHCCCCCCEEEEECCCCHHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHH
QALEKI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]