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The map label for this gene is truA

Identifier: 13358100

GI number: 13358100

Start: 665142

End: 665867

Strand: Reverse

Name: truA

Synonym: UU536

Alternate gene names: 13358100

Gene position: 665867-665142 (Counterclockwise)

Preceding gene: 13358101

Following gene: 13358098

Centisome position: 88.58

GC content: 26.86

Gene sequence:

>726_bases
ATGAATTATAAAATTAGTCTTCGTTATGATGGTAGTTTATTTTATGGATGAGCTAGACAACCACAAAAAAGAACGGTTCA
AGGGGATTTAGAAGAAATTTTTAAATCAATTTTTAAAATTAATAATATCAGGATTATTGGTTCAGGACGTACTGATAAAG
GTGTACACGCTTACGAGCAAACCTTTAGTGTTAAACATAGTGCGTTAAAATATGATTCGCACATTATTTATCAAGCACTT
TGCTCACGAACTAGTTCTGATATTCAAATTTTAGAAGTGCAAAAGGTTGATGATTCATTTCATGCTCAATATAATGCTAC
TTCTAAAACATATCAATATGTCATTAATGATTATGAATTTGATTTGTTTAGAAATAATTATGAACTTTTTGTTAATCAAA
AAATTAATGATCAAAAAATATTAGAAGCATTAGAATTGTTTGTTGGCGAACATGATTTTAAATCGTTTTCAACTAGTGAA
TTAACAATGACAATACGTCGCATTAATTGAGTTAAAATCAAAAGAGATACACACTTAATAATTTATATTAATGCAAATGG
TTTTTTAAAAAACATGGTTCGTATGATCGTTGCTTCTTGTTTAGATTATGCTTTTAATAAAATAAGTTTAGCAAAAATTC
ATGAATTATTAATATACCCCAAAAAGGGTGCTAGTATTAAATTAGCACCCGCTTGTGGATTATATTTGTATAAAGTCTAT
TATTAA

Upstream 100 bases:

>100_bases
TTTGGTGTATTTGAAGCAAGTGCATTGTTTGCTAAGTAATTTGATGAAATAACTTGTTAAACAGGTTATTTTTTATTTAT
TGGAATAGGAATTAATAATT

Downstream 100 bases:

>100_bases
TGTTTTTTTTCAACATGTTTTTTTGGAATATTAGCTATCACTTGAATACCTAATGCTCCTTTTCAAACATATACTAAAAA
GATTGGAGGACCAGATCTAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNYKISLRYDGSLFYGWARQPQKRTVQGDLEEIFKSIFKINNIRIIGSGRTDKGVHAYEQTFSVKHSALKYDSHIIYQAL
CSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVINDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSE
LTMTIRRINWVKIKRDTHLIIYINANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVY
Y

Sequences:

>Translated_241_residues
MNYKISLRYDGSLFYG*ARQPQKRTVQGDLEEIFKSIFKINNIRIIGSGRTDKGVHAYEQTFSVKHSALKYDSHIIYQAL
CSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVINDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSE
LTMTIRRIN*VKIKRDTHLIIYINANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVY
Y
>Mature_241_residues
MNYKISLRYDGSLFYG*ARQPQKRTVQGDLEEIFKSIFKINNIRIIGSGRTDKGVHAYEQTFSVKHSALKYDSHIIYQAL
CSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVINDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSE
LTMTIRRIN*VKIKRDTHLIIYINANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVY
Y

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=30.8, Blast_Score=103, Evalue=1e-23,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=26.0869565217391, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27790; Mature: 27790

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKISLRYDGSLFYGARQPQKRTVQGDLEEIFKSIFKINNIRIIGSGRTDKGVHAYEQT
CCEEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHH
FSVKHSALKYDSHIIYQALCSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVINDYEFD
HHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCCCCEEEEEECCEEEE
LFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSELTMTIRRINVKIKRDTHLIIY
EEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEEEEEEECCEEEEE
INANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVYY
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEEEECCCCEEEEEEEC
>Mature Secondary Structure
MNYKISLRYDGSLFYGARQPQKRTVQGDLEEIFKSIFKINNIRIIGSGRTDKGVHAYEQT
CCEEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHH
FSVKHSALKYDSHIIYQALCSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVINDYEFD
HHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEECCCCCEEEEEECCEEEE
LFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSELTMTIRRINVKIKRDTHLIIY
EEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEEEEEEECCEEEEE
INANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVYY
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA