Click here to switch to the map view.

The map label for this gene is pucR

Identifier: 16080295

GI number: 16080295

Start: 3328762

End: 3330357

Strand: Direct

Name: pucR

Synonym: BSU32420

Alternate gene names: 16080295

Gene position: 3328762-3330357 (Clockwise)

Preceding gene: 255767742

Following gene: 16080296

Centisome position: 78.96

GC content: 46.62

Gene sequence:

>1596_bases
ATGAATATTCTTGATGTGATGAAAATACCGGCTTTTGAAAACGCCAATTTAATCGCTGGAAAAGCAGGAGGGGAAAGAGA
GGTTCAGCATGTCAATATGATGGACGCTCCGGATATCGTAGACTTTTTGCATAAGAATGAATTGCTCGTCACCACCGCCT
ATCACCTGAAGGATCACCCGCATCAATTATCTGAACTGATTCGGCAAATGGCAAAACGCGGCTGCGCGGGCCTCGGCATT
AAAACAAAGCGCTATCTGGAAGATATCCCGAAGGAAATCATCGAACTGGCCGATTCATATGCGTTTCCGATCATTGAGCT
TCCGGAACACATCCGACTCGGCGATATTGTGAATGCCACACTCAGCCATATTCTCGATATGCGTTCTAACGAGCTGCAGC
AAGCCATTTACGCGCACAAAAAATTCACAAACCACATTATGAGCGGCAAGGGGCTGCAATCCCTCCTCAAAAAGGTGTCA
GACATCCTTCAGCTTCCCGTGCTGCTGCTTGACCAGCATGCCAAAATGCTGTCTGCGTCTCATCAGATTTCAGTTGAGAC
CGAAAAGCTGAAAGGCACCCTGAATACCGTATCCGGGCCATTTTTCACTTGTTTCTCTACAATCTCAGATCAGAAAACAT
ATTCTGTCCTGCCTATTTATAATCACGAAAAAAACTGCGGCTACCTGCTGATACCCGACATGGTGCAGGCAGGCGACAAA
GGATTGATCCTCACGATTGAACAAGCGGCCAACGTCATTTCCTTTGAACTGCTGAAGGAAAATGCGCTGAAGCAGTTCAG
CCGGCGGGCCCGCAATGAGTTTTTCAACAATTTTATTGAGCGGACCTTTTCTTCAGATGACGAAATCAAAAACCGGGCGA
AAGAATTCAAGCTGCGCTGGGATCAAAAATACATGTGCATCGCCGGCAAACTCGATCGCAATGACGAATCAATCAGCTTT
ACAGAAAATCAGCTCGCTTCCGACAGCGTATTCGAATTTCTTGAAGGTGAATTATCAGCCTTCCCGTTTCCACCTCACTT
CTTTATGAAAGGAAATGTCGGCATTATTCTAATCGAGGCGACAGACAGCTGGAGTGAAATGCACGCCTCGGTGATCAGCT
TTTTAGAACAGTTTCAAACACAGGTCAGCGCTCAATTTAAACGAACCGTGTCCTTTGGCATCAGCAATATCTGCCAAAAG
CTCATTGATGTTCCCGACGCTTTCACAGAAGCTTCTGATGCTCTGCAATCGGGGCATTTGTCAAGAAGCACCGCATTTAT
CCAGGTGTATCACGCCAAAGATGTGCCTGAGCTTCTGCGCTTGCTCCCAGTTGAGGATTTGAAGAAATTTTACAACTCCA
CGCTTCAGAGCCTCGCTGAAAAACAGCAGGAGGATCAAAGCCTGCTTCATACGCTTTCCGTCTATTTAGAGACACACTGC
CAAATTTCAGAGACGGCAAAGCGTCTGTACGTTCATCGCAATACGGTCATATACCGCCTTGAAAAATGCGAAGAGCTTCT
GGGGAAAAGCTTAAAAGATCCTGAAACAACGATGCGTTTGCGGCTTGCATTACGTATGCAGCGGCTGATCAGCTAA

Upstream 100 bases:

>100_bases
TTACTGCTACTATGATCGTATTACATCAGCAAAACCGCTAGAATTCCTATTGAAAATTATTCACCTTAACCAATGTTTTT
ATAGAAAAGGAGAATGCGCT

Downstream 100 bases:

>100_bases
AGTTTGTTACATTTTCTTACAAAACGAATCGTTTTTCGTCACTTTCAGCAATCACACCTGTCTTTTTCAGCTACTGTATC
CAATGACACCCCTCGACCGG

Product: transcriptional regulator of the purine degradation operon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHPHQLSELIRQMAKRGCAGLGI
KTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNATLSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVS
DILQLPVLLLDQHAKMLSASHQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDK
GLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFKLRWDQKYMCIAGKLDRNDESISF
TENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGIILIEATDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQK
LIDVPDAFTEASDALQSGHLSRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQEDQSLLHTLSVYLETHC
QISETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLIS

Sequences:

>Translated_531_residues
MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHPHQLSELIRQMAKRGCAGLGI
KTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNATLSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVS
DILQLPVLLLDQHAKMLSASHQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDK
GLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFKLRWDQKYMCIAGKLDRNDESISF
TENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGIILIEATDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQK
LIDVPDAFTEASDALQSGHLSRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQEDQSLLHTLSVYLETHC
QISETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLIS
>Mature_531_residues
MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHPHQLSELIRQMAKRGCAGLGI
KTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNATLSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVS
DILQLPVLLLDQHAKMLSASHQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDK
GLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFKLRWDQKYMCIAGKLDRNDESISF
TENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGIILIEATDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQK
LIDVPDAFTEASDALQSGHLSRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQEDQSLLHTLSVYLETHC
QISETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLIS

Specific function: Activates the expression of pucFG, pucH, pucI, pucJKLM and guaD, while it represses pucABCDE and its own expression

COG id: COG2508

COG function: function code TQ; Regulator of polyketide synthase expression

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CdaR family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCR_BACSU (O32138)

Other databases:

- EMBL:   AL009126
- PIR:   D70016
- RefSeq:   NP_391122.1
- ProteinModelPortal:   O32138
- SMR:   O32138
- EnsemblBacteria:   EBBACT00000000798
- GeneID:   937221
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32420
- NMPDR:   fig|224308.1.peg.3248
- GenoList:   BSU32420
- GeneTree:   EBGT00050000001503
- HOGENOM:   HBG338282
- OMA:   CQISETA
- ProtClustDB:   CLSK872814
- BioCyc:   BSUB:BSU32420-MONOMER
- GO:   GO:0006350
- InterPro:   IPR012914

Pfam domain/function: PF07905 PucR

EC number: NA

Molecular weight: Translated: 60514; Mature: 60514

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHP
CCCHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHHCCCH
HQLSELIRQMAKRGCAGLGIKTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNAT
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHH
LSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVSDILQLPVLLLDQHAKMLSAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDK
CCEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECHHHCCCCC
GLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFKLRW
CEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DQKYMCIAGKLDRNDESISFTENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGIILIEA
CCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHCHHCCCCCCCCEEEECCEEEEEEEC
TDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQKLIDVPDAFTEASDALQSGHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
SRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQEDQSLLHTLSVYLETHC
CCCEEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QISETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLIS
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNILDVMKIPAFENANLIAGKAGGEREVQHVNMMDAPDIVDFLHKNELLVTTAYHLKDHP
CCCHHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHHCCCH
HQLSELIRQMAKRGCAGLGIKTKRYLEDIPKEIIELADSYAFPIIELPEHIRLGDIVNAT
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHH
LSHILDMRSNELQQAIYAHKKFTNHIMSGKGLQSLLKKVSDILQLPVLLLDQHAKMLSAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HQISVETEKLKGTLNTVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDK
CCEEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECHHHCCCCC
GLILTIEQAANVISFELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNRAKEFKLRW
CEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DQKYMCIAGKLDRNDESISFTENQLASDSVFEFLEGELSAFPFPPHFFMKGNVGIILIEA
CCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHCHHCCCCCCCCEEEECCEEEEEEEC
TDSWSEMHASVISFLEQFQTQVSAQFKRTVSFGISNICQKLIDVPDAFTEASDALQSGHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
SRSTAFIQVYHAKDVPELLRLLPVEDLKKFYNSTLQSLAEKQQEDQSLLHTLSVYLETHC
CCCEEEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QISETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLIS
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136; 12029039