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The map label for this gene is pucJ

Identifier: 16080296

GI number: 16080296

Start: 3330502

End: 3331851

Strand: Direct

Name: pucJ

Synonym: BSU32430

Alternate gene names: 16080296

Gene position: 3330502-3331851 (Clockwise)

Preceding gene: 16080295

Following gene: 16080297

Centisome position: 79.0

GC content: 50.52

Gene sequence:

>1350_bases
GTGAAAAAAAGATCGTTTAAGGTGTTTACACTTAGCCTGCAGCATGTGCTCGCCATGTATGCAGGTGCGATTTTGGTGCC
CCTGCTTGTCGGCAGGGCGCTGAATGTAACAACTGAACAGCTGTCCTACCTATTGGCGATTGACCTGTTAACATGCGGCG
TCGCTACGCTTTTGCAAACCTTGCGCGGAACATACATCGGCATCGGGCTTCCCGTCATGCTTGGCAGTTCATTTGTGGCC
GTCACCCCAATGATTGCAATCGGCAGCAATTACGGCATTCACGCGATTTATGGTTCCATCATTGCGGCCGGTGTGTTCAT
CTTTTTGTTTGCGCGTTTTTTCGGCAAGCTGACTGTGTTATTTCCTCCTGTCGTAACAGGTACCGTTGTGACCTTGATCG
GCCTTTCACTTGTTCCGACAGGTGTGAAAAATATGGCTGGCGGCGAAAAAATAAACGGCAGCGCCAACCCCGAATACGGT
TCGCTCGAAAACCTTCTTCTCTCTGTCGGTGTCCTTGTTCTGATCTTAGTCCTCAACCGCTTTCTCAAAGGATTTGCCCG
AACACTGTCCGTTCTGATCGGCATCGCCGCCGGAACAGCGGCAGCAGCGATCATGGGCAAAGTCAGCTTTTCTTCTGTTA
CAGAAGCACCGTTCTTCCAAATCCCGAAGCCTTTTTACTTCGGAGCCCCTGCGTTTGAAATCGGTCCGATCTTAACGATG
CTGATCGTCGGAATCGTCATTATTGTGGAATCAACCGGCGTTTTTTACGCAATCGGAAAGATATGCGGCAGACCTTTAAC
TGACAAAGATCTCGTCAAAGGCTATCGAGCGGAAGGAATCGCCATTCTCATCGGCGGGCTGTTCAATGCCTTTCCTTATA
ATACCTTTGCGCAAAACGCCGGCTTATTGCAGCTGACCAAAGTGAAAACAAGAAACATCGTCGTTACCGCAGGCTGTATC
CTTGTCTGTCTCGGACTGATTCCGAAGATTGCCGCCCTTGCATCTGCCGTTCCTGCTGCTGTACTTGGCGGCGCGACCGT
CGTCATGTTCGGCATGGTGATTGCCTCCGGCGTCAAAATGCTCAGCACAGCGGATCTCAAAAATCAATACCACCTCCTGA
CCATCGCATGCTCAATCGCACTCGGCATCGGCGCCAGCACGGCCCCCGGGATTTTTGCTGAATTCCCCGCTCCGATCCGG
ATTCTGGTCAGTGACGGCACCATCACCGGAAGCCTAACCGCCATCTTTTTGAATCTATTTTTCAGCTTGCGCGACAAAAA
AGAATTAACAGCACAGCAAACAGAACTTCCGGTATTAGAACATACACTCGCATTAGAAAAAGAGGTGTAA

Upstream 100 bases:

>100_bases
TCAGCAATCACACCTGTCTTTTTCAGCTACTGTATCCAATGACACCCCTCGACCGGAACGATATTATGTTACTAAAGTTC
ACATACAGGGAGGGACTATA

Downstream 100 bases:

>100_bases
AAAAGATGAAGGAACAGCACAATGCCTTACAGCTAATGATGCTGGGGCTTCAGCACATGCTTGCCATGTATGCGGGAGCC
ATTCTCGTTCCGTTGATCGT

Product: uric acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKRSFKVFTLSLQHVLAMYAGAILVPLLVGRALNVTTEQLSYLLAIDLLTCGVATLLQTLRGTYIGIGLPVMLGSSFVA
VTPMIAIGSNYGIHAIYGSIIAAGVFIFLFARFFGKLTVLFPPVVTGTVVTLIGLSLVPTGVKNMAGGEKINGSANPEYG
SLENLLLSVGVLVLILVLNRFLKGFARTLSVLIGIAAGTAAAAIMGKVSFSSVTEAPFFQIPKPFYFGAPAFEIGPILTM
LIVGIVIIVESTGVFYAIGKICGRPLTDKDLVKGYRAEGIAILIGGLFNAFPYNTFAQNAGLLQLTKVKTRNIVVTAGCI
LVCLGLIPKIAALASAVPAAVLGGATVVMFGMVIASGVKMLSTADLKNQYHLLTIACSIALGIGASTAPGIFAEFPAPIR
ILVSDGTITGSLTAIFLNLFFSLRDKKELTAQQTELPVLEHTLALEKEV

Sequences:

>Translated_449_residues
MKKRSFKVFTLSLQHVLAMYAGAILVPLLVGRALNVTTEQLSYLLAIDLLTCGVATLLQTLRGTYIGIGLPVMLGSSFVA
VTPMIAIGSNYGIHAIYGSIIAAGVFIFLFARFFGKLTVLFPPVVTGTVVTLIGLSLVPTGVKNMAGGEKINGSANPEYG
SLENLLLSVGVLVLILVLNRFLKGFARTLSVLIGIAAGTAAAAIMGKVSFSSVTEAPFFQIPKPFYFGAPAFEIGPILTM
LIVGIVIIVESTGVFYAIGKICGRPLTDKDLVKGYRAEGIAILIGGLFNAFPYNTFAQNAGLLQLTKVKTRNIVVTAGCI
LVCLGLIPKIAALASAVPAAVLGGATVVMFGMVIASGVKMLSTADLKNQYHLLTIACSIALGIGASTAPGIFAEFPAPIR
ILVSDGTITGSLTAIFLNLFFSLRDKKELTAQQTELPVLEHTLALEKEV
>Mature_449_residues
MKKRSFKVFTLSLQHVLAMYAGAILVPLLVGRALNVTTEQLSYLLAIDLLTCGVATLLQTLRGTYIGIGLPVMLGSSFVA
VTPMIAIGSNYGIHAIYGSIIAAGVFIFLFARFFGKLTVLFPPVVTGTVVTLIGLSLVPTGVKNMAGGEKINGSANPEYG
SLENLLLSVGVLVLILVLNRFLKGFARTLSVLIGIAAGTAAAAIMGKVSFSSVTEAPFFQIPKPFYFGAPAFEIGPILTM
LIVGIVIIVESTGVFYAIGKICGRPLTDKDLVKGYRAEGIAILIGGLFNAFPYNTFAQNAGLLQLTKVKTRNIVVTAGCI
LVCLGLIPKIAALASAVPAAVLGGATVVMFGMVIASGVKMLSTADLKNQYHLLTIACSIALGIGASTAPGIFAEFPAPIR
ILVSDGTITGSLTAIFLNLFFSLRDKKELTAQQTELPVLEHTLALEKEV

Specific function: Uptake of uric acid

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Homo sapiens, GI44680148, Length=170, Percent_Identity=31.1764705882353, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI40316845, Length=170, Percent_Identity=31.1764705882353, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI44680145, Length=298, Percent_Identity=27.1812080536913, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI44680143, Length=315, Percent_Identity=26.984126984127, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI87082181, Length=420, Percent_Identity=42.3809523809524, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1790087, Length=425, Percent_Identity=33.1764705882353, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082178, Length=432, Percent_Identity=32.6388888888889, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081818, Length=397, Percent_Identity=28.7153652392947, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788843, Length=389, Percent_Identity=30.8483290488432, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558856, Length=199, Percent_Identity=30.6532663316583, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17542262, Length=184, Percent_Identity=28.2608695652174, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17542260, Length=183, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17541904, Length=392, Percent_Identity=24.4897959183673, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=183, Percent_Identity=27.3224043715847, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356175, Length=177, Percent_Identity=32.2033898305085, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCJ_BACSU (O32139)

Other databases:

- EMBL:   AL009126
- PIR:   E70016
- RefSeq:   NP_391123.1
- ProteinModelPortal:   O32139
- EnsemblBacteria:   EBBACT00000002094
- GeneID:   937096
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32430
- NMPDR:   fig|224308.1.peg.3249
- GenoList:   BSU32430
- GeneTree:   EBGT00050000001001
- HOGENOM:   HBG470187
- OMA:   WVCATAG
- PhylomeDB:   O32139
- ProtClustDB:   CLSK2752499
- BioCyc:   BSUB:BSU32430-MONOMER
- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043
- PANTHER:   PTHR11119:SF3
- PANTHER:   PTHR11119
- TIGRFAMs:   TIGR00801
- TIGRFAMs:   TIGR03173

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 47101; Mature: 47101

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11b9d7e8)-; HASH(0x12438fa8)-; HASH(0x11224b60)-; HASH(0x10b073e4)-; HASH(0xf16f30c)-; HASH(0x11d39d98)-; HASH(0x11b27c10)-; HASH(0x116c7e50)-; HASH(0x12267028)-; HASH(0x11ab7a3c)-; HASH(0x122d9358)-; HASH(0x11f78da4)-; HASH(0x11e59390)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHCCHHHHHHHHHHCCH
>Mature Secondary Structure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HHHCCHHHHHHHHHCCHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136