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The map label for this gene is fixA

Identifier: 16519983

GI number: 16519983

Start: 151198

End: 152055

Strand: Direct

Name: fixA

Synonym: NGR_a01220

Alternate gene names: 16519983

Gene position: 151198-152055 (Clockwise)

Preceding gene: 16519984

Following gene: 16519982

Centisome position: 28.2

GC content: 59.44

Gene sequence:

>858_bases
ATGCACATCGTAGTCTGTATCAAGCAAGTCCCGGATTCGGCGCAGATACGCGTCCATCCGGTGACGAACACGATCATGCG
CCAAGGCGTGCCGACTATCATCAACCCTTACGACCTGTTTGCCCTCGAACAGGCACTCCAAGTGCGTGACCACCATGGCG
GCGAGGTCACGGTGCTCACCATGGGCCCCCCCATGGCCGAAGAATCTTTACGCAAGGCGCTCACTTATGGCGCAGACCGC
GCGATACTCCTGACCGACCGGCACTTTGCCGGCTCCGACACGCTGGCGACTTCATTTGCTCTTTCTCAGGCAATCGCGAA
GATTGGCAATAGCTTTGGATCGCCTGATATCGTCTTCACAGGCAAGCAGACGATCGACGGCGATACCGCCCAGGTCGGCC
CCGGAATAGCCAAGCGCCTCGACCTCGTGCAGTTGACCTATGTCGCAAAGGTCACTTCCATTGATCTCCGCACGCGCGCG
ATTACGGTCGAGCGTCGCGCGGAAGGCGGGACGCACGTCCTGAAGAGCGAATTGCCTTGCCTGATCACCATGCTGGAAGG
ATCGAATGCCATTCGCCGCGGCTCACTTGATGATGCCTTGCGGGCCGCGCGCAGCGAAATCGTCAAGTGGAATGCGGTGG
AGGCTGGCATTGAAGACATCACAAAATGCGGGCTGCGCGGTTCGCCGACGGTCGTCAAGCGGGTCTTTGCCCCTACCCCG
CGCGAGCAAAAAGCGGTGCAGATCGACACCGTCGAGAAGGCAATGCAGGACGTAGCTAACGAACTGGTCACTTCAATCTT
CGCCCATCAGCCGGCACTGGAGCACGAGCTCTCCTCCACTCGTAGCGTGAGCAGATAG

Upstream 100 bases:

>100_bases
AATTCATGGGCTTCTTGGACCACAGTTGGTTGGCATGGTTGGTGCTTGGAAGGGAGGCGAAAGACGTCATTTTACACGCG
ATCGGAGCTAAAGAGACCTC

Downstream 100 bases:

>100_bases
GAGCAGATTATGGGAACTCGAAAAAGTAAAGCATCACCACCGGCCCAAGCCCGTGCCAGCATGAAAAAGGACCTGCCTGA
GCAATTCAGAGACTACCGGC

Product: nitrogen fixation protein FixA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEQALQVRDHHGGEVTVLTMGPPMAEESLRKALTYGADR
AILLTDRHFAGSDTLATSFALSQAIAKIGNSFGSPDIVFTGKQTIDGDTAQVGPGIAKRLDLVQLTYVAKVTSIDLRTRA
ITVERRAEGGTHVLKSELPCLITMLEGSNAIRRGSLDDALRAARSEIVKWNAVEAGIEDITKCGLRGSPTVVKRVFAPTP
REQKAVQIDTVEKAMQDVANELVTSIFAHQPALEHELSSTRSVSR

Sequences:

>Translated_285_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEQALQVRDHHGGEVTVLTMGPPMAEESLRKALTYGADR
AILLTDRHFAGSDTLATSFALSQAIAKIGNSFGSPDIVFTGKQTIDGDTAQVGPGIAKRLDLVQLTYVAKVTSIDLRTRA
ITVERRAEGGTHVLKSELPCLITMLEGSNAIRRGSLDDALRAARSEIVKWNAVEAGIEDITKCGLRGSPTVVKRVFAPTP
REQKAVQIDTVEKAMQDVANELVTSIFAHQPALEHELSSTRSVSR
>Mature_285_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEQALQVRDHHGGEVTVLTMGPPMAEESLRKALTYGADR
AILLTDRHFAGSDTLATSFALSQAIAKIGNSFGSPDIVFTGKQTIDGDTAQVGPGIAKRLDLVQLTYVAKVTSIDLRTRA
ITVERRAEGGTHVLKSELPCLITMLEGSNAIRRGSLDDALRAARSEIVKWNAVEAGIEDITKCGLRGSPTVVKRVFAPTP
REQKAVQIDTVEKAMQDVANELVTSIFAHQPALEHELSSTRSVSR

Specific function: May play a role in a redox process involved in nitrogen fixation

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family

Homologues:

Organism=Homo sapiens, GI4503609, Length=209, Percent_Identity=31.1004784688995, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI62420877, Length=196, Percent_Identity=31.1224489795918, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI87081682, Length=252, Percent_Identity=26.984126984127, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367123, Length=214, Percent_Identity=27.5700934579439, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25141345, Length=214, Percent_Identity=32.7102803738318, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321646, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24651147, Length=186, Percent_Identity=30.6451612903226, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24651145, Length=186, Percent_Identity=30.6451612903226, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXA_RHISN (Q53210)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444103.1
- ProteinModelPortal:   Q53210
- SMR:   Q53210
- GeneID:   962188
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01220
- HOGENOM:   HBG716515
- ProtClustDB:   CLSK2299090
- GO:   GO:0006810
- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR21294
- PIRSF:   PIRSF000090
- SMART:   SM00893

Pfam domain/function: PF01012 ETF

EC number: NA

Molecular weight: Translated: 30909; Mature: 30909

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEQALQVRDHHGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEE
MGPPMAEESLRKALTYGADRAILLTDRHFAGSDTLATSFALSQAIAKIGNSFGSPDIVFT
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
GKQTIDGDTAQVGPGIAKRLDLVQLTYVAKVTSIDLRTRAITVERRAEGGTHVLKSELPC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHCCCCE
LITMLEGSNAIRRGSLDDALRAARSEIVKWNAVEAGIEDITKCGLRGSPTVVKRVFAPTP
EEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
REQKAVQIDTVEKAMQDVANELVTSIFAHQPALEHELSSTRSVSR
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEQALQVRDHHGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEE
MGPPMAEESLRKALTYGADRAILLTDRHFAGSDTLATSFALSQAIAKIGNSFGSPDIVFT
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
GKQTIDGDTAQVGPGIAKRLDLVQLTYVAKVTSIDLRTRAITVERRAEGGTHVLKSELPC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHCCCCE
LITMLEGSNAIRRGSLDDALRAARSEIVKWNAVEAGIEDITKCGLRGSPTVVKRVFAPTP
EEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
REQKAVQIDTVEKAMQDVANELVTSIFAHQPALEHELSSTRSVSR
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424