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The map label for this gene is fixB

Identifier: 16519982

GI number: 16519982

Start: 152065

End: 153174

Strand: Direct

Name: fixB

Synonym: NGR_a01230

Alternate gene names: 16519982

Gene position: 152065-153174 (Clockwise)

Preceding gene: 16519983

Following gene: 16519981

Centisome position: 28.36

GC content: 59.01

Gene sequence:

>1110_bases
ATGGGAACTCGAAAAAGTAAAGCATCACCACCGGCCCAAGCCCGTGCCAGCATGAAAAAGGACCTGCCTGAGCAATTCAG
AGACTACCGGCACGTCTGGGTTTTCATGGAGCTGGAACGCGGCCATGTCCACCCGGTCTCGTTCGAACTTCTCGGCGAAG
GCCGCAGACTTGCCGACCAACTGGGGGTTCAGCTCGCAGGTATTATTCTCGGGCCTCCGGGGGATTCTACCTTGCACGCC
GCTGCTGAGGCCTTCGCCTATGGTGCGGATCTGGTGTATCTCGTCGAGGAGCCGCTGCTCACGGATTATAGAAACGAGCC
TTTCACCAAAGCACTGACAGATCTGGTCATTACCCATAAGCCTGAGATTCTGCTTCTCGGTGCGACGACGCTGGGTCGCG
ACCTCGCCGGTTCCGTGGCGACCACACTGCTGACGGGGCTCACAGCCGACTGCACCGGACTTGATGTCGACGAGGACGGT
TCGCTTGCAGCTACTCGGCCCACGTTCGGTGGCTCGTTGCTTTGCACGATTTATACGCTCAATTGCCGGCCCCAAATGGC
AACTGTGCGGCCAAGGGTCATGCGCATGCCACAGCGGTCAAACAAGCCGATCGGGCGGGTAATCCGGCACGATTGGCGGA
TGTTGGAGGAAGATATCGTCACGAAGGTCGTCGGTTTCCTTTCCGACGACCGATCGGGCAACGCCAATCTCGCCTATGCG
GATGTGGTGGTTGCCGGCGGGCTGGGCCTCAGCAATGCAGAGAACCTGCTGCTTATCAAAGACCTCGCGCTGACGATCGG
CGGTGACTACGGCTGTTCCAGGCCGCTCGTCCAAAAGGGCTGGATGCCAGCTGATCGGCAGATCGGCCAAACTGGCAAAA
CGATCCGCCCGAAGCTCTATATAGCGGCTGGAATATCAGGCGCGGTCCAGCACCGCGTCGGTGTCGAGGGAGCTGACCTG
ATTGTCGCTATCAACACCGATCCACACGCGCCCATCTTCGATTTCGCCCATGTCGGTGTTGTCACCGATGCTGTCAGCTT
TCTGCCGGCGTTGACGGAAGCTTTCACCAAACGGTTGGCGCCGGCCAATCGTCTGAGAGTTGTGAACTGA

Upstream 100 bases:

>100_bases
GGACGTAGCTAACGAACTGGTCACTTCAATCTTCGCCCATCAGCCGGCACTGGAGCACGAGCTCTCCTCCACTCGTAGCG
TGAGCAGATAGGAGCAGATT

Downstream 100 bases:

>100_bases
GGTAAAACCTATGACCGAAGAAAGATTCGACGCCATCGTCGTCGGTGCCGGTATGTCTGGAAACGCGGCTGCATACACCA
TGGCAAGTCGCGGTCTAAAA

Product: nitrogen fixation protein FixB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MGTRKSKASPPAQARASMKKDLPEQFRDYRHVWVFMELERGHVHPVSFELLGEGRRLADQLGVQLAGIILGPPGDSTLHA
AAEAFAYGADLVYLVEEPLLTDYRNEPFTKALTDLVITHKPEILLLGATTLGRDLAGSVATTLLTGLTADCTGLDVDEDG
SLAATRPTFGGSLLCTIYTLNCRPQMATVRPRVMRMPQRSNKPIGRVIRHDWRMLEEDIVTKVVGFLSDDRSGNANLAYA
DVVVAGGLGLSNAENLLLIKDLALTIGGDYGCSRPLVQKGWMPADRQIGQTGKTIRPKLYIAAGISGAVQHRVGVEGADL
IVAINTDPHAPIFDFAHVGVVTDAVSFLPALTEAFTKRLAPANRLRVVN

Sequences:

>Translated_369_residues
MGTRKSKASPPAQARASMKKDLPEQFRDYRHVWVFMELERGHVHPVSFELLGEGRRLADQLGVQLAGIILGPPGDSTLHA
AAEAFAYGADLVYLVEEPLLTDYRNEPFTKALTDLVITHKPEILLLGATTLGRDLAGSVATTLLTGLTADCTGLDVDEDG
SLAATRPTFGGSLLCTIYTLNCRPQMATVRPRVMRMPQRSNKPIGRVIRHDWRMLEEDIVTKVVGFLSDDRSGNANLAYA
DVVVAGGLGLSNAENLLLIKDLALTIGGDYGCSRPLVQKGWMPADRQIGQTGKTIRPKLYIAAGISGAVQHRVGVEGADL
IVAINTDPHAPIFDFAHVGVVTDAVSFLPALTEAFTKRLAPANRLRVVN
>Mature_368_residues
GTRKSKASPPAQARASMKKDLPEQFRDYRHVWVFMELERGHVHPVSFELLGEGRRLADQLGVQLAGIILGPPGDSTLHAA
AEAFAYGADLVYLVEEPLLTDYRNEPFTKALTDLVITHKPEILLLGATTLGRDLAGSVATTLLTGLTADCTGLDVDEDGS
LAATRPTFGGSLLCTIYTLNCRPQMATVRPRVMRMPQRSNKPIGRVIRHDWRMLEEDIVTKVVGFLSDDRSGNANLAYAD
VVVAGGLGLSNAENLLLIKDLALTIGGDYGCSRPLVQKGWMPADRQIGQTGKTIRPKLYIAAGISGAVQHRVGVEGADLI
VAINTDPHAPIFDFAHVGVVTDAVSFLPALTEAFTKRLAPANRLRVVN

Specific function: May play a role in a redox process involved in nitrogen fixation

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family

Homologues:

Organism=Homo sapiens, GI4503607, Length=128, Percent_Identity=47.65625, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI189181759, Length=128, Percent_Identity=47.65625, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787990, Length=332, Percent_Identity=30.1204819277108, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786226, Length=338, Percent_Identity=28.4023668639053, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17506929, Length=140, Percent_Identity=47.8571428571429, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325261, Length=195, Percent_Identity=35.8974358974359, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17136898, Length=331, Percent_Identity=30.2114803625378, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24652801, Length=331, Percent_Identity=30.2114803625378, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXB_RHISN (Q53209)

Other databases:

- EMBL:   Z68203
- EMBL:   U00090
- RefSeq:   NP_444102.1
- ProteinModelPortal:   Q53209
- SMR:   Q53209
- GeneID:   962189
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a01230
- HOGENOM:   HBG615986
- ProtClustDB:   CLSK2299089
- GO:   GO:0006810
- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF000089
- SMART:   SM00893

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha

EC number: NA

Molecular weight: Translated: 39851; Mature: 39720

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRKSKASPPAQARASMKKDLPEQFRDYRHVWVFMELERGHVHPVSFELLGEGRRLADQ
CCCCCCCCCCCHHHHHHHHHCCHHHHHCCEEEEEEEEECCCCCCCEEHEECCCCHHHHHH
LGVQLAGIILGPPGDSTLHAAAEAFAYGADLVYLVEEPLLTDYRNEPFTKALTDLVITHK
HCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHHCCC
PEILLLGATTLGRDLAGSVATTLLTGLTADCTGLDVDEDGSLAATRPTFGGSLLCTIYTL
CCEEEEECHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEE
NCRPQMATVRPRVMRMPQRSNKPIGRVIRHDWRMLEEDIVTKVVGFLSDDRSGNANLAYA
CCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DVVVAGGLGLSNAENLLLIKDLALTIGGDYGCSRPLVQKGWMPADRQIGQTGKTIRPKLY
EEEEECCCCCCCCCCEEEEEEEHHEECCCCCCCCCHHHHCCCCCCHHHCCCCCCCCCEEE
IAAGISGAVQHRVGVEGADLIVAINTDPHAPIFDFAHVGVVTDAVSFLPALTEAFTKRLA
EEECCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PANRLRVVN
CCCCEEECC
>Mature Secondary Structure 
GTRKSKASPPAQARASMKKDLPEQFRDYRHVWVFMELERGHVHPVSFELLGEGRRLADQ
CCCCCCCCCCHHHHHHHHHCCHHHHHCCEEEEEEEEECCCCCCCEEHEECCCCHHHHHH
LGVQLAGIILGPPGDSTLHAAAEAFAYGADLVYLVEEPLLTDYRNEPFTKALTDLVITHK
HCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHHCCC
PEILLLGATTLGRDLAGSVATTLLTGLTADCTGLDVDEDGSLAATRPTFGGSLLCTIYTL
CCEEEEECHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEE
NCRPQMATVRPRVMRMPQRSNKPIGRVIRHDWRMLEEDIVTKVVGFLSDDRSGNANLAYA
CCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DVVVAGGLGLSNAENLLLIKDLALTIGGDYGCSRPLVQKGWMPADRQIGQTGKTIRPKLY
EEEEECCCCCCCCCCEEEEEEEHHEECCCCCCCCCHHHHCCCCCCHHHCCCCCCCCCEEE
IAAGISGAVQHRVGVEGADLIVAINTDPHAPIFDFAHVGVVTDAVSFLPALTEAFTKRLA
EEECCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PANRLRVVN
CCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424