Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 15668731

GI number: 15668731

Start: 487102

End: 487929

Strand: Reverse

Name: nirB [C]

Synonym: MJ0551

Alternate gene names: 15668731

Gene position: 487929-487102 (Counterclockwise)

Preceding gene: 15668732

Following gene: 15668730

Centisome position: 29.31

GC content: 32.37

Gene sequence:

>828_bases
TTGGTGAAAAGGATGCACTGCAATATAAACTTAAAATATGGCGTTATAATGAAAAAAGATTGTTATACATTAAGAATCTC
ATTAAAACCCGGATTTATAAATGCTGAGCAGTTAAAGGCAATAGCCTATGTTATTGAAAATTTTGGAGATAACAAAGCCC
ATATAACAACAAGGCAAGGTATAGAGTTTAAAATATCTCCAGAACATTTGGAAGAAGTAGAGAAAATTCTAAATAATGTG
GGGTTAAACTTAGGTTCTACTGGAAATAGAGTTAGGCAAGTAGTGTCATGTATTGGCTTAGAGTGCTACAATGCTATTGG
TGACTCTGTCTCTTTGGCAAGGAGAATTCATGAGGAGTTTGAAGGAGTTTGGGTTCCAAGAAAGGTGAAGATAAATGTTA
GTGGTTGCCCAAATTCATGCACATTTCATAGGTTTTGTGATATTGGGATATGTTATAGATACAAAATAACCATAAACAAA
GAGATTTGCACAAATTGTGGAAAATGTAAAGATTTTTGTGATTTAAATGCTATAGATTGGGAACGAAAAATAATAAAAGA
TAATTGCACTGGAGAAGGAAAATGCACTGGCTTATGTAATGCCTTTAAAGCTGAGAGAGTTATTAGCATATTCGTTGGAG
GAAAAGGAGGAAGAATATATAAGGAGGGAAAACACCTAATAGATTTAAAAAATGAGGATGATGTCTTATTTGTTATTGAT
GAATTGATAAGCTTATATGCAAAGTTTGGAAAGGGTAGGATGGCAGATTTTGTTGAAAACTATGGGATTGAAAACTTAAG
AAATAACATAAAAGAGTTGATAAAATGA

Upstream 100 bases:

>100_bases
ATAGTTGTTGAAAGACCTAAAATTGATTATCCAGTTTGTTTTGATGATATAGATGAGCTTATAAAATACATAGCTAATTT
AAAAATTTAATTTTATAATT

Downstream 100 bases:

>100_bases
ACCAAATTATTAATGATAATTATAGGGAATTCTTAAAAAAATTAAGAGAGAGACATTTAAAAAAGAGAAAAGTTAAAGAT
AAAAATAAACCTATTGCTGT

Product: nitrite reductase NirA

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQGIEFKISPEHLEEVEKILNNV
GLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEFEGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINK
EICTNCGKCKDFCDLNAIDWERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVID
ELISLYAKFGKGRMADFVENYGIENLRNNIKELIK

Sequences:

>Translated_275_residues
MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQGIEFKISPEHLEEVEKILNNV
GLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEFEGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINK
EICTNCGKCKDFCDLNAIDWERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVID
ELISLYAKFGKGRMADFVENYGIENLRNNIKELIK
>Mature_275_residues
MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQGIEFKISPEHLEEVEKILNNV
GLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEFEGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINK
EICTNCGKCKDFCDLNAIDWERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVID
ELISLYAKFGKGRMADFVENYGIENLRNNIKELIK

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y551_METJA (Q57971)

Other databases:

- EMBL:   L77117
- PIR:   G64368
- RefSeq:   NP_247530.1
- ProteinModelPortal:   Q57971
- GeneID:   1451416
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0551
- NMPDR:   fig|243232.1.peg.567
- TIGR:   MJ0551
- OMA:   FKAERVI
- ProtClustDB:   CLSK2517621
- BioCyc:   MJAN243232:MJ_0551-MONOMER
- InterPro:   IPR017896
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 31189; Mature: 31189

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51379 4FE4S_FER_2; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQG
CCEEEEEEEEEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
IEFKISPEHLEEVEKILNNVGLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEF
EEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINKEICTNCGKCKDFCDLNAIDW
CCEECCEEEEEEECCCCCCCCCHHEECCEEEEEEEEEECHHHHCCCCCCCHHCCCCCCCH
ERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVID
HHHHHHCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCEEEECCCEEEEECCCCCEEEEHH
ELISLYAKFGKGRMADFVENYGIENLRNNIKELIK
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHC
>Mature Secondary Structure
MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQG
CCEEEEEEEEEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCC
IEFKISPEHLEEVEKILNNVGLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEF
EEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINKEICTNCGKCKDFCDLNAIDW
CCEECCEEEEEEECCCCCCCCCHHEECCEEEEEEEEEECHHHHCCCCCCCHHCCCCCCCH
ERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVID
HHHHHHCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCEEEECCCEEEEECCCCCEEEEHH
ELISLYAKFGKGRMADFVENYGIENLRNNIKELIK
HHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087