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The map label for this gene is cbiJ

Identifier: 15668732

GI number: 15668732

Start: 487940

End: 488689

Strand: Reverse

Name: cbiJ

Synonym: MJ0552

Alternate gene names: 15668732

Gene position: 488689-487940 (Counterclockwise)

Preceding gene: 15668733

Following gene: 15668731

Centisome position: 29.35

GC content: 30.67

Gene sequence:

>750_bases
ATGAACCTACTGTTAATGGGAGGAACTAAGGATAGTGTTGAAATTGGTAAAAAGCTTAGGGATTTGGGAGATTTATTTAT
TTTATACACTTCAACAACTGATTATGGTGGAAAATTAGGGGAGGAATTTGCCAACAAAGTGATAACAAAACCTTTAGATA
AAAATGAGTTGAAAGAGGTTATAAAGAAATATAATATAGATATTTTAGTCGATGCCACTCATCCATTTGCAATAAATGCA
AGCAAAAATGCCATTGAAGTTTGTAAAGAGCTTAATATAAAGTATGTAAGATTTGAGAGAAAAGAGGAAAAGATAAATCA
TCCAAATATAATATATGTTAAAGATTTTGAAGAAGCTGCAAGATTGGCTAAAAAAGCAAATAAAGTCTTTCACATGGCAG
GAATTAAGAATTTAAAGATGGTTGTTGATATTGTTGGGAAAGATAAGGTTATAGCAAGAGTTCTCCCTATATCTGTAAGT
GAGGCATTAAAGATTTTACCTCAAAAGCAGATTGTAGCTATGTATGGGACTTTTTCTAAGGAGCTTAACAAATATTTAAT
AAGGGATTATAACTGTGATGTGATAATAACTAAAGATAGTGGGGAGAGTGGGGGTTTTAAAGAGAAAGTTTATGGGGCTT
TAGAAGCTGAAGCCAAGGTTATAGTTGTTGAAAGACCTAAAATTGATTATCCAGTTTGTTTTGATGATATAGATGAGCTT
ATAAAATACATAGCTAATTTAAAAATTTAA

Upstream 100 bases:

>100_bases
CGAAATTTTTTATTGTAAAATGTCAAAAGAATAAAAGATTTACAATTAAGAGATTTTTTGACTTTTTGAGCATATACAAA
AGTCTATTTATGGTGAAGTC

Downstream 100 bases:

>100_bases
TTTTATAATTTTGGTGAAAAGGATGCACTGCAATATAAACTTAAAATATGGCGTTATAATGAAAAAAGATTGTTATACAT
TAAGAATCTCATTAAAACCC

Product: cobalamin biosynthesis protein CbiJ

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEVIKKYNIDILVDATHPFAINA
SKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAARLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVS
EALKILPQKQIVAMYGTFSKELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDEL
IKYIANLKI

Sequences:

>Translated_249_residues
MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEVIKKYNIDILVDATHPFAINA
SKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAARLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVS
EALKILPQKQIVAMYGTFSKELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDEL
IKYIANLKI
>Mature_249_residues
MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEVIKKYNIDILVDATHPFAINA
SKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAARLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVS
EALKILPQKQIVAMYGTFSKELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDEL
IKYIANLKI

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIJ_METJA (Q57972)

Other databases:

- EMBL:   L77117
- PIR:   H64368
- RefSeq:   NP_247531.1
- ProteinModelPortal:   Q57972
- GeneID:   1451417
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0552
- NMPDR:   fig|243232.1.peg.568
- TIGR:   MJ0552
- HOGENOM:   HBG711334
- OMA:   PYIRFER
- ProtClustDB:   CLSK876245
- BioCyc:   MJAN243232:MJ_0552-MONOMER
- InterPro:   IPR003723
- TIGRFAMs:   TIGR00715

Pfam domain/function: PF02571 CbiJ

EC number: 1.3.1.54

Molecular weight: Translated: 28177; Mature: 28177

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEV
CEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
IKKYNIDILVDATHPFAINASKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAA
HHHCCCEEEEECCCCEEEECCCHHHHHHHHCCCEEEEEECHHHHCCCCCEEEEECHHHHH
RLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVSEALKILPQKQIVAMYGTFSK
HHHHHHHHEEECCCCCCCEEEEEECCCCCEEHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
ELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDEL
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
IKYIANLKI
HHHHHCCCC
>Mature Secondary Structure
MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEV
CEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
IKKYNIDILVDATHPFAINASKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAA
HHHCCCEEEEECCCCEEEECCCHHHHHHHHCCCEEEEEECHHHHCCCCCEEEEECHHHHH
RLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVSEALKILPQKQIVAMYGTFSK
HHHHHHHHEEECCCCCCCEEEEEECCCCCEEHHHHCCCHHHHHHHCCHHHHHHHHHHHHH
ELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDEL
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
IKYIANLKI
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087