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The map label for this gene is uraA

Identifier: 16273146

GI number: 16273146

Start: 1295680

End: 1296924

Strand: Reverse

Name: uraA

Synonym: HI1227

Alternate gene names: 16273146

Gene position: 1296924-1295680 (Counterclockwise)

Preceding gene: 16273147

Following gene: 16273145

Centisome position: 70.86

GC content: 41.53

Gene sequence:

>1245_bases
ATGACAAACCAAATCCCTCCTTCCCTAGCGGAAAATCAAAGCAAATTAAAACAATCTTTCGTTGGCCTACAAATGCTTTT
TGTTGCATTTGGTGCGCTAGTACTCGTGCCATTAATTACAGGACTTGATTCCAATACGGCATTATTAACCGCTGGCGTTG
GCACCCTACTCTTCCAATTTTGCACAGGCAAACAAGTGCCTATTTTCTTGGCATCATCTTTTGCCTTTATCGCGCCCATT
CAATATGGCGTACAAACTTGGGGAATTGCGACTACGATGGGCGGTTTAGCGTTCACTGGTCTTGTTTATTTCGCCTTGAG
CACTTTAGTAAAATTACGGGGAGCAGAAGCATTACAACGTTTTTTCCCACCAGTGGTTGTTGGGCCAGTAATTATTATCA
TTGGCATGGGGCTTGCACCTATTGCGGTGGATATGTCCTTAGGCAAAAACAGTGCCTATGCTTATAACGACGCAGTATTA
GTTTCAATGGTAACGCTTCTTACCACTTTATCTGTGGCAGTATTTGCCAAAGGATTGATGAAACTTATTCCAATAATGTT
CGGTATTACCGCAGGCTATATTTTGTGTTTATTTCTAGGTTTAATTAATTTCCAACCAGTGATTGATGCGCCTTGGTTCA
GTTTGCCCAAATTAACGACACCTGAATTTAACCTAGAAGCCATTCTTTATATGTTACCAATTGCCATTGCCCCCGCCGTA
GAACACGTTGGCGGTATTATGGCAATCAGCTCTGTAACAGGTAAAGACTTTTTAAAAAAACCAGGATTACACCGCACTTT
ATTAGGCGATGGCATCGCTACCGCTGCTGCCTCTTTAGTTGGTGGCCCGCCTAATACGACTTATGCGGAAGTTACTGGTG
CTGTGATGCTAACACGCAACTTTAACCCAAATATTATGACTTGGGCTGCAGTTTGGGCTATTGCGATTTCATTCTGTGGA
AAAGTAGGCGCATTCCTATCAACCATTCCAACTATTGTAATGGGTGGAATTATGATGCTTGTTTTCGGTTCAATCGCAGT
TGTGGGCATGAGTACCTTAATTCGCGGAAAAGTTGATGTTACCGAAGCTCGTAATCTTTGTATTATTTCTGTCGTAATGA
CATTTGGTATCGGCAATATGTTTGTAGATGTGGGTAACGTATCACTAAAAGGTATCAGCTTGTGTGCGATTGTTGCTATC
ATCTTAAACCTTGTTTTACCGAAAGCAAAAAATGAAGTAGAATAA

Upstream 100 bases:

>100_bases
CAAAACAAGACGGCATTTAAGCCGTCTTTTTTCCGTGCGTTGAAACACAAGAAAAACTGACCGCACTTTTAAGTTAATTC
AAACAGAGAGTTGAAACTAA

Downstream 100 bases:

>100_bases
GCCCCATTTCAAAGGGTTAAATTATCATTTAGCCCTTTCTTACTTAATCTGTACTACATAAACATTTGAATAAGCAACTC
CCCTTACCTATTCATCAAAT

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTNQIPPSLAENQSKLKQSFVGLQMLFVAFGALVLVPLITGLDSNTALLTAGVGTLLFQFCTGKQVPIFLASSFAFIAPI
QYGVQTWGIATTMGGLAFTGLVYFALSTLVKLRGAEALQRFFPPVVVGPVIIIIGMGLAPIAVDMSLGKNSAYAYNDAVL
VSMVTLLTTLSVAVFAKGLMKLIPIMFGITAGYILCLFLGLINFQPVIDAPWFSLPKLTTPEFNLEAILYMLPIAIAPAV
EHVGGIMAISSVTGKDFLKKPGLHRTLLGDGIATAAASLVGGPPNTTYAEVTGAVMLTRNFNPNIMTWAAVWAIAISFCG
KVGAFLSTIPTIVMGGIMMLVFGSIAVVGMSTLIRGKVDVTEARNLCIISVVMTFGIGNMFVDVGNVSLKGISLCAIVAI
ILNLVLPKAKNEVE

Sequences:

>Translated_414_residues
MTNQIPPSLAENQSKLKQSFVGLQMLFVAFGALVLVPLITGLDSNTALLTAGVGTLLFQFCTGKQVPIFLASSFAFIAPI
QYGVQTWGIATTMGGLAFTGLVYFALSTLVKLRGAEALQRFFPPVVVGPVIIIIGMGLAPIAVDMSLGKNSAYAYNDAVL
VSMVTLLTTLSVAVFAKGLMKLIPIMFGITAGYILCLFLGLINFQPVIDAPWFSLPKLTTPEFNLEAILYMLPIAIAPAV
EHVGGIMAISSVTGKDFLKKPGLHRTLLGDGIATAAASLVGGPPNTTYAEVTGAVMLTRNFNPNIMTWAAVWAIAISFCG
KVGAFLSTIPTIVMGGIMMLVFGSIAVVGMSTLIRGKVDVTEARNLCIISVVMTFGIGNMFVDVGNVSLKGISLCAIVAI
ILNLVLPKAKNEVE
>Mature_413_residues
TNQIPPSLAENQSKLKQSFVGLQMLFVAFGALVLVPLITGLDSNTALLTAGVGTLLFQFCTGKQVPIFLASSFAFIAPIQ
YGVQTWGIATTMGGLAFTGLVYFALSTLVKLRGAEALQRFFPPVVVGPVIIIIGMGLAPIAVDMSLGKNSAYAYNDAVLV
SMVTLLTTLSVAVFAKGLMKLIPIMFGITAGYILCLFLGLINFQPVIDAPWFSLPKLTTPEFNLEAILYMLPIAIAPAVE
HVGGIMAISSVTGKDFLKKPGLHRTLLGDGIATAAASLVGGPPNTTYAEVTGAVMLTRNFNPNIMTWAAVWAIAISFCGK
VGAFLSTIPTIVMGGIMMLVFGSIAVVGMSTLIRGKVDVTEARNLCIISVVMTFGIGNMFVDVGNVSLKGISLCAIVAII
LNLVLPKAKNEVE

Specific function: Transport of uracil in the cell

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Homo sapiens, GI44680143, Length=432, Percent_Identity=23.8425925925926, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI44680145, Length=429, Percent_Identity=23.3100233100233, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI44680148, Length=431, Percent_Identity=21.8097447795824, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI40316845, Length=431, Percent_Identity=21.8097447795824, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788843, Length=399, Percent_Identity=36.5914786967419, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87081818, Length=413, Percent_Identity=34.8668280871671, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI87082181, Length=408, Percent_Identity=24.5098039215686, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082178, Length=325, Percent_Identity=24, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=417, Percent_Identity=23.9808153477218, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=410, Percent_Identity=21.9512195121951, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356175, Length=421, Percent_Identity=23.2779097387173, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): URAA_HAEIN (P45117)

Other databases:

- EMBL:   L42023
- PIR:   D64111
- RefSeq:   NP_439383.1
- GeneID:   950172
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1227
- NMPDR:   fig|71421.1.peg.1173
- TIGR:   HI_1227
- HOGENOM:   HBG470187
- OMA:   YACIRGQ
- ProtClustDB:   CLSK869935
- BioCyc:   HINF71421:HI_1227-MONOMER
- InterPro:   IPR006042
- InterPro:   IPR006043
- PANTHER:   PTHR11119:SF3
- PANTHER:   PTHR11119
- TIGRFAMs:   TIGR00801

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 43573; Mature: 43442

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb3e85e0)-; HASH(0xe2501e4)-; HASH(0xdbaa31c)-; HASH(0xe2a1ad4)-; HASH(0xda1fe8c)-; HASH(0xdeb9424)-; HASH(0xddef4a8)-; HASH(0xe04505c)-; HASH(0xe1d5e9c)-; HASH(0xb2511cc)-; HASH(0xe088a08)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQIPPSLAENQSKLKQSFVGLQMLFVAFGALVLVPLITGLDSNTALLTAGVGTLLFQF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
CTGKQVPIFLASSFAFIAPIQYGVQTWGIATTMGGLAFTGLVYFALSTLVKLRGAEALQR
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FFPPVVVGPVIIIIGMGLAPIAVDMSLGKNSAYAYNDAVLVSMVTLLTTLSVAVFAKGLM
HCCHHHHHHHHHHHHCCHHHHEEEHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
KLIPIMFGITAGYILCLFLGLINFQPVIDAPWFSLPKLTTPEFNLEAILYMLPIAIAPAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EHVGGIMAISSVTGKDFLKKPGLHRTLLGDGIATAAASLVGGPPNTTYAEVTGAVMLTRN
HHHCHHHHHHCCCCHHHHHCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHCEEEEEEC
FNPNIMTWAAVWAIAISFCGKVGAFLSTIPTIVMGGIMMLVFGSIAVVGMSTLIRGKVDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TEARNLCIISVVMTFGIGNMFVDVGNVSLKGISLCAIVAIILNLVLPKAKNEVE
HHHCCHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
TNQIPPSLAENQSKLKQSFVGLQMLFVAFGALVLVPLITGLDSNTALLTAGVGTLLFQF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
CTGKQVPIFLASSFAFIAPIQYGVQTWGIATTMGGLAFTGLVYFALSTLVKLRGAEALQR
HCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
FFPPVVVGPVIIIIGMGLAPIAVDMSLGKNSAYAYNDAVLVSMVTLLTTLSVAVFAKGLM
HCCHHHHHHHHHHHHCCHHHHEEEHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
KLIPIMFGITAGYILCLFLGLINFQPVIDAPWFSLPKLTTPEFNLEAILYMLPIAIAPAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EHVGGIMAISSVTGKDFLKKPGLHRTLLGDGIATAAASLVGGPPNTTYAEVTGAVMLTRN
HHHCHHHHHHCCCCHHHHHCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHCEEEEEEC
FNPNIMTWAAVWAIAISFCGKVGAFLSTIPTIVMGGIMMLVFGSIAVVGMSTLIRGKVDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TEARNLCIISVVMTFGIGNMFVDVGNVSLKGISLCAIVAIILNLVLPKAKNEVE
HHHCCHHHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800