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The map label for this gene is llaDCHIA [H]

Identifier: 91789207

GI number: 91789207

Start: 3547989

End: 3548813

Strand: Direct

Name: llaDCHIA [H]

Synonym: Bpro_3351

Alternate gene names: 91789207

Gene position: 3547989-3548813 (Clockwise)

Preceding gene: 91789206

Following gene: 91789208

Centisome position: 68.23

GC content: 47.15

Gene sequence:

>825_bases
TTGCCTACAAATGAACTTTTCCCTGTAACGAATCATGCACAACCCGTTTTACGCTGGGCCGGCAGCAAGCGTCGCATCAC
CCCCATTCTTTCAGCTTATGCCCCCCCGAAATACGAACGCTACTTCGAACCTTTTGTAGGCTCGGGAGCACTCTTCTTCT
GCTTAAGGCCGCCGCTAGCTTTCATTGGAGACGTTAACCCGGAAGTCACGTCAACGTATCAGGCTCTAAAAGATGCCCCC
TCGCAGGTAGCCCAACACCTTACAGAAATTCCTAAAACGAAAGACGCTTACCTACTGCTGCGTCAACTCCGTCCGGCTGA
TCTGACAACAGCGCAGCGAGCAGCGAGACTAATTTTCCTAATGAAAAGTTGCTTTAATGGCGTATACCGCACAAATGCGT
CCGGACAATTCAATGTGCCATTCGGGTCCACTATCTACAAACTACCAACGTCAGAAGAACTTCAAAGTGTTTCCGACGCT
TTGAAATTAACGCAGGTTAGAACGGGCGACTATAAAAGTTGGCTTGATATCGCAGGCGTGGGAGATTTCATATATTTGGA
TCCACCTTATAGTTGCAGCTCAAGATATAGGGGAGAGTATGGCTATGACGCTATCTTCGGAGACGTGCAACTAGAAGAGT
TGTTAACCTGTTGCAAAGCACTTCAGAATCGGGGGGCAAAAGTCATGCTTTCGTACAAGAATAACAAACAAGTCGTGAAT
GCACTAAAGGATTGGCGCATTTTGAATCTCGCTGTACCTAGAAGCGTTAATGCGAATAGTATGGGCCGAACACCAGCCGC
CGAAATACTAGCAATGAATTACTAA

Upstream 100 bases:

>100_bases
GTCACAGACATTGTTGACCACAAGCCGGGGAAAACCGTGTTCAATCGGGTGACGGGGATGAAAGATAGCTGGGGAAAGTA
GCGTGATTGTCGATACGCAG

Downstream 100 bases:

>100_bases
AACACTGACGGGAGATGAGATGAAGTTACGTATTGCCGTGCTAAGCGACTTGCATGCCACCGGCCATGCCGAAAATAATC
CATCATCCTCTTACATGCGT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPTNELFPVTNHAQPVLRWAGSKRRITPILSAYAPPKYERYFEPFVGSGALFFCLRPPLAFIGDVNPEVTSTYQALKDAP
SQVAQHLTEIPKTKDAYLLLRQLRPADLTTAQRAARLIFLMKSCFNGVYRTNASGQFNVPFGSTIYKLPTSEELQSVSDA
LKLTQVRTGDYKSWLDIAGVGDFIYLDPPYSCSSRYRGEYGYDAIFGDVQLEELLTCCKALQNRGAKVMLSYKNNKQVVN
ALKDWRILNLAVPRSVNANSMGRTPAAEILAMNY

Sequences:

>Translated_274_residues
MPTNELFPVTNHAQPVLRWAGSKRRITPILSAYAPPKYERYFEPFVGSGALFFCLRPPLAFIGDVNPEVTSTYQALKDAP
SQVAQHLTEIPKTKDAYLLLRQLRPADLTTAQRAARLIFLMKSCFNGVYRTNASGQFNVPFGSTIYKLPTSEELQSVSDA
LKLTQVRTGDYKSWLDIAGVGDFIYLDPPYSCSSRYRGEYGYDAIFGDVQLEELLTCCKALQNRGAKVMLSYKNNKQVVN
ALKDWRILNLAVPRSVNANSMGRTPAAEILAMNY
>Mature_273_residues
PTNELFPVTNHAQPVLRWAGSKRRITPILSAYAPPKYERYFEPFVGSGALFFCLRPPLAFIGDVNPEVTSTYQALKDAPS
QVAQHLTEIPKTKDAYLLLRQLRPADLTTAQRAARLIFLMKSCFNGVYRTNASGQFNVPFGSTIYKLPTSEELQSVSDAL
KLTQVRTGDYKSWLDIAGVGDFIYLDPPYSCSSRYRGEYGYDAIFGDVQLEELLTCCKALQNRGAKVMLSYKNNKQVVNA
LKDWRILNLAVPRSVNANSMGRTPAAEILAMNY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=263, Percent_Identity=26.2357414448669, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30682; Mature: 30550

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTNELFPVTNHAQPVLRWAGSKRRITPILSAYAPPKYERYFEPFVGSGALFFCLRPPLA
CCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCHH
FIGDVNPEVTSTYQALKDAPSQVAQHLTEIPKTKDAYLLLRQLRPADLTTAQRAARLIFL
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MKSCFNGVYRTNASGQFNVPFGSTIYKLPTSEELQSVSDALKLTQVRTGDYKSWLDIAGV
HHHHHHHHEECCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
GDFIYLDPPYSCSSRYRGEYGYDAIFGDVQLEELLTCCKALQNRGAKVMLSYKNNKQVVN
CCEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
ALKDWRILNLAVPRSVNANSMGRTPAAEILAMNY
HHHCCEEEEEECCCCCCCCCCCCCCHHHHEEECC
>Mature Secondary Structure 
PTNELFPVTNHAQPVLRWAGSKRRITPILSAYAPPKYERYFEPFVGSGALFFCLRPPLA
CCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCHH
FIGDVNPEVTSTYQALKDAPSQVAQHLTEIPKTKDAYLLLRQLRPADLTTAQRAARLIFL
HHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
MKSCFNGVYRTNASGQFNVPFGSTIYKLPTSEELQSVSDALKLTQVRTGDYKSWLDIAGV
HHHHHHHHEECCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
GDFIYLDPPYSCSSRYRGEYGYDAIFGDVQLEELLTCCKALQNRGAKVMLSYKNNKQVVN
CCEEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
ALKDWRILNLAVPRSVNANSMGRTPAAEILAMNY
HHHCCEEEEEECCCCCCCCCCCCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]